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CRIMIC CR01541

Gene sni

CG10964

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:8242877..8242879	7D22	FBti0210424	86438

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGCCAGGCAACTAACGGTTATTGC	TGCAGGTAAACTAACCGCTGTCATCG

Images

Construct Design Sequences

sgRNA sequence	GCTCGACGGTTGTGAGTCACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATCGAACGGCCGTTATCCT	CTCACAACCGTCGAGCTACA	CACCGGTTTTGTTGGATTACTG	ATGTGGATGCTGGAAGGGTG

 

Amplicon 5'

Amplicon 3'

GATCGAACGGCCGTTATCCTGGCCGATTTGGGCGCTATGCCGGCATTGTTGAAGAGCACATTGAGGCCTTGGTCCTTGGTCACGCCCTCGATGTCGGCGACTAGCTTGTCATAGGCATCGAAATTTCTCAAATCTGCACGGAAAATATATTTATATGCGAGAGTGTTTAGCGGCTGGTAAAACATAATGCTTACCAATCTCAAGTATGTGTATGTTCGAGTGGTTCTTGGCTAGATCCTCCAGCTCCTGTGGGCCAAATTGGGAAAGACAAAATGTTTGAATAAGAAGCACATTATTAGTAAAAAAGCAGTTTATATATGAATAATTTTCCAGTTAAATTACTGTAATTTTTAGAGGCAAATTTGGTATTTCATGAGAGTTTAAATAGCGCGCCCAACGAGCGCGTTAGTGCCATAATAACCGATAGACGATAAGTGCTGCTGGTGTGACCGAACAATAGTCGGCCCAGCAATCGACAAGCCAATCGACGTCTCCCTTTCGCACGCTCGTACGAAAGTACAAAAGCTATTGCAAAAGTTGGCTCCGCTTATTCGTTTCGTGCTTTCGCGAGTGCCGAGAGCCGCTACAATACACGCTTAGCAGTTTTTACATTTCCGCTTCGACTACAACAACATTCACTACCCGCCGTTGATCCTTGTTTTCTGTCTGATTTACGTGGAGCACCTACCAACAAGCAACAAAATAATGGCGCCCGTGCAAGGTGAGTTTCACAGTTAATTCGATTCCAATTGAACCATTCAATTTATGCATGCCCATTTGGACGATTAAAAAGCGGTTTGTAGAAATAGTGGCTCTTGTGGATTGTTCGCGTAGCTTCTGTAGTTTCTGTAGCTCGACGGTTGTGAG

CACCGGTTTTGTTGGATTACTGGATTGTCTTAGTAGAGAGACAACGCAAAATAAGAAGAAAACATCTCTCACCTTTGCCTGCTCGCGATTCCGGCAGGTGGTAAATAGATGCTGCGGCGGCTGGGGAAGATTGAGCAGCGCCTTGACCAGGCCCAGACCCAATCCTCGATTGCAGCCGGTTATCAGGATGGAGTTCATGTTTTTTTTTTCCGAAGATCCGCGATCGAGTTGCTCGAAATCTTATCGCGTCGTACTTGAGATAAAACCAATAAAGGGCGGCACTACTATACGGGTTCCGTGTGTTCTGTGGATTTCACCAGACCCGCAGCCAGATCTTTCCGTGCTTATCAATCGACTTTATTGCCGTAACACAAGCGTGAAAATAGTGAAATGTTAGGAAAAATTTTGACGGCCGGCTGTTTCGTTCGGACAGCAGCAGTGTTACCAACCATCCGCGAGTTATCGATACACTTGCGCATTGCCAAACGTGCGTTTCGCACATTTCCAATTGGCAAAGGGCAGAACAACTGTTGTGATGTTCAAATCCCAAATGGAATTTCGCAATCCAATGGATGCTCCTTTATGGCAAATCCATTTTATCCCAAACATTCTCGTTTTTGCGGTACGCGCTCTTGCGCCCTCTCTCTTTCTCGCTGCTTCTGTTACGTAGCCGTTTATCGGCGCCTCTGCCTGCCCACCGCTTTCGCAGCATTCCCGGCCGAACCAGCGAACGTGTTTGTGTTGTGTGTTCCGCCGTCATTTTTCTGCACCCTTTTCGCGAATAGTTTCGTTTCGCCTCCAGCTGGTAGAGTGAAACGCCAAACGTTGAAGAAGGGGAAAGGCCAACAAGATGAACTTGTGCAATTCGAGATTCTCCGTTACGTTCGTGCGGCAGTGCTCGACGATTTTAACGTCTCCTTCGGCTGGCATTATACAAAACAGAGGCTCACTGACAACAAAGGTTCCCCATTGGATTTCCAGTAGTCTCAGCTGTGCCCATCACACGTTTCAGCGAACTATGAACTTGACGGGACAGCGAGGATCACGCGACAGTACTGGAGCTACCGGTGGGAATGCTCCAGCCGGATCCGGTGCCGGCGCACCACCACCCTTCCAGCATCCACAT

 

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