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CRIMIC CR01545

Gene Rab39

CG12156

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:7735550..7735572	7B7	FBti0210425	86439

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGATGGAGGAGAACGTGGTGGATTC	CGTGCAGCTACGAACGTTTGTGTATC

Images

Construct Design Sequences

sgRNA sequence	GTGTGCTCGCCTGATAATGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATACGAAGCGGACGGTGTT	GCGAGCACACAAACATGCA	ACAACACACAAGTCCAAAATAGG	CGCAGAATCTCGGCTACGTA

 

Amplicon 5'

Amplicon 3'

AATACGAAGCGGACGGTGTTCACGGAAAACAAACGTAAAAAGAAAATACAGTGCGTACTAGCGATGGGTTTTTCTCTGCACGAGACAGCCTAGAACGAAAATGAACACGCACGTCTTAATTTATGTGTCAAAGCACTGTACAAGGCCAAATCTTTTTGTGTAATTATTGTAAATTTATTAGCTTAATATGAAAGATTATTTTATTTTTTTTGTCATATTTTCTCAGAACAATTTAAACTAACTACATTTGTATGCATTCATCGTGTCACGCGAAATCGCGTTCTTTGACCATCTCTATTGGGCGGCGGTGCCAGACTGGCGGTGTTGCCATTAGCCGGTTACCGTTTTCAAGCCAAGTCTAGCTAGTTTTTGTGTACCGCCGAAAAATACATCTAAGCTTGTTAAAAAATCTGAAAACTGCCAAATTGATGTGAGGACACTATTTTTATTAAATAATTTAGAAGATATAACTGTTTAATAATGGTAATGAAATAATTTCATTGTGGCTACTATCGATTACTTTTGCTGGTCTGGCAACGTTGTCATTATCACACACACGCGCACGCATATGAGAAAAAAACACAAAACAAAACAAAATTCGAAGGAATTTGAATTCTCGAATATTTATAATTTAACAACCACCAGCGACAGCCGGAAATATCTCATTGCAAAGCCAGTAATCGTGTGAAGAACAGAAAGTTTCGATTTGCAATACAACTGCAGGAAATAAGAAGAAAAACGAGCAGCATAAGCAAATCAACGATTTGCGTATCAAACTTTACAAAGGGAAGGCAGATTTTTTGCTGCGGATTCAATTCCAAGAGACAAATCCCTCGAATTCGGCTTCGGAATGGTGGAACCCATTTTCGAGTATCAATTTCGACTGATTTTAATCGGCGACAGCACCGTGGGCAAGAGCTCGCTGCTCAAATTCTTCACAGACGGCAAATTCGCCGAGGTGCGTATGTCCAACGATTTTGGCCACTGTACTTGGACGCTTGGCCCACTGTGCGATCTTCGTGAGATGCAGTTAATGCGTTCATTACATAATTAAGTGATTGCATTGGGCGAGATACAACAAAAAATAGACAAAGGGAGTGGATTTTAAAAAGATGCTGCCTTAGTCACCTTGTCAGTGTGTGTGTGTGCTGGTGTGCATGTTTGTGTGCTCGC

ACAACACACAAGTCCAAAATAGGACCAGGGATACTCAAATAACTGCGCGGGAAATTTAAAAACATAGAGATATCATGAATTGTGTGGAATCAAAAGTGTTCTCAAAAGCATTACAGCTCTAAACTGCGTTTCTTTTTACTGCCTCCCAAGAATCCACCTAATCATAAATAACACAATATTTACATTTTGCAGTTATCCGATCCCACAGTTGGCGTCGACTTCTTTGCCCGCCTCATCGAAATGAAGGACGGCACACAGATCAAGCTCCAGCTGTGGGACACTGCTGGCCAAGAGCGCTTCCGTTCGATCACCAAGTCCTACTACCGGAACTCGGTTGGCGTGCTGCTGGTCTACGACATATCGAATCACGCCAGCTTCGAGCACATACCGCTGTGGATGATGGAGGCACAGCGTCACATTGAGCCCCACCGCCCCGTTTTCGCATTGGTCGGCTGCAAACTGGACCTCATCAATGCGGGCGGACACCGGGAGGTGACCACAGAGGAGGCACAAAAATTTGCCAAACAGCACGGTCTGCATTTCGTTGAGACATCGGCCCGGTCTGGTGCCAATGTGGAGGAGGCATTTCGCATGGTCACCCAGGAGGTGTACGCCCGCATTCGATCCGGCGAATATAAGGCGGAGGATGGATGGGACGGCATCAAGTCTGGCTTTTCGCGACCCAATAGTCTGGACTTTAATCTCGTCGTAGCAGAGCCGGAAAAGTCATCATGTTGTTGATTCACCCCGACCTCATTGTCTATTATTTTCAATACTGTTTATGTACATTGATTTGTATCGTATGACCCACCAGTACTCCTTTTCCCCCCTAAACACCAATTTGTTGACTCTTTAATCTTGTTTAATCGTCTAGTTTAGCTAAAAAACCAAAACAGTTACGTATATTTACACAATGCGTTTTTTTTCTAATTACATATAGTTAATCGCTTTTAAGTTAATATTTAGTTCGGTGACTCACGGTAATTAGTGAAGATTGAGTTTGAACCCCTCTTTCCAAATTATGTTTACTTCGCCCCTATAATTGATAGCCAGTCACACGAGGTATACGTAGCCGAGATTCTGCG

 

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