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CRIMIC CR01546

Gene rdgB

CG11111

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:13770609..13770629	12C2	FBti0210426	86440

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAGGGTAGTTCCAAGAATCGTGTGC	AATGCTTGGCACTGACCTCATCGAAC

Images

Construct Design Sequences

sgRNA sequence	GTTGGATCGTGTTGGATGGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTTTGGCCACCTCCACTAT	ATTTGGTAAGACGCAGCACA	ATCCAACACGATCCAACGAC	CTCAGGCTGGAGAGAATGCC

 

Amplicon 5'

Amplicon 3'

GCTTTGGCCACCTCCACTATCCTATCATCCACTTCAGCCTCACCACATACACTTGCTAAATTGTAAAAAGTATAAGTTTGCATAAAGATAAAGCAAAAAATGAATGATGTCATTCACATATTGAATTTGAAGGATATGTAGTTGAGAAAGGCAGTGAAACCTTTCGTATTCATTCGGACATCAATCCATCGACTGGAAAATGAACCAATGTAATCAATGTATTTATTATTCGTATTCATCCATTTCAGACAGCGTCTCGGAGCCGTACGTCAGCACGGCGGCCACCGCCGCCTCCACAACGGGCAGCGAGCGAAAGAAGTCCGCTCCGGCTGTGCCGCCTATTGTCACCCAGCAGCCGCCGAGCGCCGAGGCCAGTTCGGATGAGGAGGGCGAGGAGGAGGAGGATGACGACGAGGACGAGAACGATGCCATTGGCACGGGCGTGGATCTGTCAGCCAACCAAGGCGGATCCGCGCAGCGCTCGCGCTCCCAAAGTATTCAGATGGCCCAGAAGGGCAAGTTCGGGTCAAAGGGTGCCCTTCACTCGCCGGTGGGATCTGCCCATAGCTTCGATCTCCAGGTGAGCACGGCCAGATGATTATCTATTATCTTTGTCCTGTGTCCTTTGGTGGATTTCGTGTGTTAATTCTGCAAATGAATGTTTAAACAAGTTCGGTGTTCCTTAATGCAAATTACTATTGTGTGTTTGTTATTAACATGACTGAAAAGTCAGGGAAATTTCCCATTTAAAAGGGCTACTTTAAAAGATATCTACTAGAGTTTTCTATAGAATAAGAATAACAATCTTACTTAATTGCTTACTGAATCCAGCAAGAAAAATATGTCTTTAACTTTGACAAAACTAGTTTAATTCGAATTTCAAAGCGTTTCAAGTTTGATGTGCAATTTTTTGTTGCACTTAAACTTCAATTTTAATCTAGTTTTCAATATTTTCATTCAAAAACACCTTTATTCTGTCTCTGTGTTCAATTTTCTGTGTGTTATGTGTGTCGCTATGTCCTTTAAATGTGCTGCGTCTTACCAAAT

ATCCAACACGATCCAACGACAACAACTACAACACAAACGACACAAATCGATAAAAAAAAAACCGAATAAATTAAATATATATAAAAATTATAAACCAAATTACTCGCTCAATTAAATCGAAAACAATCCAAAATCAGTCCAAAAAGCCGCATGCAAAGACAGCGCCACGCAGACATGTAAGTTCCTCTCGCCCTCTCTGTCGCTCCCTTTTACGCCCTCTCTCTTTCTTTTTCATTACTAGCAATTGCCCAGCTAATAGCGCGTCATTATAGATAGTATAAAACCTCCAATGACCCATATTAAAGCCTCCTACCCCTCATCCATTCCCTTAGTAATTTGCTTAGTCGCAGTTTTTGATTAGCCAAGTTCCATTAGCTTAGCTTAGCCATTCGATGCCGTTTTTTGTAGAACAGATTCGAAGAAATATACATATATCCATGCCTATAAATAAACAATTGTGTCTATATGCAAGTATTAATTCAATTTACGCACACCTCAGGTGGCTAACTGGCGTATGGAGCGATTGGAAGTGGACTCCAAATCCAATTCGGATGAGGAATTCTTTGATTGCCTGGGTAAGCATTGCATTATGAATATTCTAGAGAAACTAAAATAATAGAGAAACTAATATGTATTTTCATTTCATAGACACCAATGAGACGAACTCGCTGGCCAAGTGGAGCTCGCTGGAGCTGCTTGGCGAGGGCGACGACAGTCCGCCGCCACATGGCGGACCCTCTAGTGCAGCATCGGTGGGTGGGCGTGGCAACTCGCGGCAAGAGGACAGCATATTCAATCAGGACTTTCTGATGCGCGTGGCCTCGGAGCGCGGCAACAAGCGGCAGTTACGTTCCTCGGCCAGCGTGGATCGCAGTCACGATTCATCGCCGCCGGGATCGCCGAGTACACCGTCGTGTCCCACAACCATTCTGATCCTGGTTGTCCATGCGGGCAGCGTTTTGGATGCGGCCAGCGAGCTGACCGCCAAGAAATCCGATGTGACCACATTCCGTGGCTCCTTCGAGGCGGTTATGCGACAGCACTATCCCAGCCTCCTCACCCATGTGACCATCAAGATGGTGCCGTGCCCCTCAATATGCACCGACGCCCTGGGCATTCTCTCCAGCCTGAG

 

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