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CRIMIC CR01547

Gene NAAT1

CG3252

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:5410849..5405238	4F9	FBti0212931	91259

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	CGGCAGCTCATCAAGTAACGTGGAAT	CTGCTGCACAACTTGGAAAAGTTCCC

Images

Construct Design Sequences

sgRNA sequence	TCGAGCCGTACGCTAAGTTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACGCGGTAACTGTTTTGCC	TACGGCTCGAGATCAGCGAA	GTTTTCGGTTCATTCTTCGGC	CTGCTTGACTTTGGCGGTTC

 

Amplicon 5'

Amplicon 3'

AACGCGGTAACTGTTTTGCCGCTTACATATTTGCTCATTCGGGGAAAAAGCTGTTTAAATAAAACAATAACTCAGCTGGCCGAGTGGGCGGGCAATTACGAACGAAGTTAAATGGCAAAGAAACTGCATCATCATAGATACATAGATATGTATCCGCCAGTACGGTATCTATGAAATAGCCGAAACCCATGTGTGTTTGCTTATCCCATTTTATCTGCAAAGCCAAAGGCTCTCATTACCTTTCCATTAAGAAATTTATATATATAGTATCTATATCTATACATAAATATCTACCAGCTGGCTGTTGAACCTCAAACAAACTCGCATAATTGTGGGGTTTTTTTTTCTGCGTGCTGGGAAAAATTCTATCTTGGGAATCGCCTTAATTGCATTTGCCATCAATAAAACCTATGCTGACCGCACTTTAATGAGGCTTTGTTTTGGTCTGTCAATGTCACTCTGTGCGGGGGGATAACCCTGTAATTTAAATCAACTATAAGTATAAATCTTAACGATCTGTGCAGCATCTGCATTTTGCATGCAAACAGGCTGAGTTCTAAGGGGAATTACCTATAACCATATTATAGATATATATTCATATATATGTATATATTTATAGAAACTAAAGTTTTTAATCACCCGCAAGAACCAATTCTATATATCTTCGTATCTTTGTAACTTTTTTTTTTTACAATACTTTCTCGGAAATGCTGTAATATATAATAGAGGGCTGCAAAGTGAACCTGTCACTCCACCGAGCACTCGCCTATATATATTATAGCTTCGTCATATGTTTATGGCTGCTGCAGTGAAAACTGGTTTCCAGCTCTTCGGCCATATAGCGAGTATAGTACAGTATAGTACGGTATAGTACGGTATAGTACACTATAGCCTATTATAGTATAGCTTTCGCTGATCTCGAGCCGTA

GTTTTCGGTTCATTCTTCGGCTGAAAATAGCTCTAAGATACATTTATGTGTCGCTGGGTATCGTAAAGCGTATAAGATATGCATGGAGTAACCGGCTGACAAAGTCTTTCACCCCACATTGGGGTGGACCGTAAATCGCATATTCGTTTGTCGGTCGATTGGCCTGCAATTAAGGTCTAACAGCCTTAATGATCATAAAGTCATTTGGCGATTGTAAAGCCAGAAACACTGAGCGAATTGCAAGGACGGTTGTGAAGAAGGGCCAATCAAAGCATAATTTCGGGGTAAAAGTCCATTGAAGTTTTATAAAGTTTATTGCATGGGAAATGTTTCTTCACAGTGTACATTGTGAAACTTTCATGCACCACACGGCGTATACTTAATGTTCCAACTGTTGAACACTGCCTTCTTCTAATCTGAATCGCTGGCCACTTGAGTTCTTCAAGTGCCACTCGAAAGCAATTCCATTAGGGATACTATTAATTGCCTGATTTCATGGTCTAACACTTACTTCCGTTGATGCAGCATTGGTGGCTCCATTTCCACTTCCATTTGAGCTGCCATTCGATGGCTGAACTCCTTTCAATTCCATTTTTCTAGCCACTTGTTAATTTTCCCGGTTAATGAAAACAGTTGACGGCTACTCGCGTTTTGCTTATTTAATTACTAATTATTTATTTTTTTTTTTTTTGTTAATAGTAATTAGCACATTGCATTTGGCCAGCCCTTGGTTACGGTTGGTTGGGTTAGCGTCCAACTGCAGTTTCAGGGTTTAGTTTTCTGTAATATTTTCGTAATTTGGGCTGTGGATTTTGGCTACTTGGCGGTGCTGGAGGCGCGCTTGTGGTGCCGTGAATGCCGCGGCTGGAACTGAGAATATTGCGAGTGCCGGCCGCTTTAAGAACCGCCAAAGTCAAGCAG

 

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