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CRIMIC CR01549

Gene Pdp

CG12151

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:7733743..7733765	7B7	FBti0210428	86442

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCCACCATTCCGAAGCCGAATTCGA	GCGATGACTAGAAACTTGTCGTTGGG

Images

Construct Design Sequences

sgRNA sequence	TTTGTTTATAGGAAAACACTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCGTCTGGGGATCAAGAAC	TGAACGACGTCGACCCGA	CGGGACTTTCTTGAAAGCCC	TTTTCTCATATGCGTGCGCG

 

Amplicon 5'

Amplicon 3'

TGCGTCTGGGGATCAAGAACGCCCAACACTGCGCCACAATCACCCGTGCTGGCCACGTGCATCTGCAGTCCCTCAATGTGAACCAAACAGGCAACGGCTCCTGGAAAATAAACACATTTATAGTTTAGAAATGAAGCCCCTATGAAGAGGAAAGTATGAAACGGGATGGCAACTTATATACATAAATAAATGGATACATCACCCACCAGAAAGGGCCACGTTCATGGTGCGTACATCGTTGCTGGTCAGAGCCTCCTGCGAGATCTCTTCATCCAGCTGGAGGAAGGCGTTCACCAGTTCACTGGATACATCCCGTTGCGGTGTCTCTAACAATTGATTGACATACTTGAGGAAACTAGCCTCGTACATGGGCTTGATCATGCTAACAAAGTCCACGTTGTCATTGTGGCACTTGAGGAACGACTGGCTGTCGGCACCCTGCTTCATCTGCTCCCGGAGCACCTGGCGCGGCAGCGTGGCCGCCGAAACATAGCGAAGCAGTCGCTTGGACACCACTTGGCCACATGCTGCTCCCGCATGCCCGTCGAAAATGCCACAAATGAAGCCGTTGCGGTGCAGGAAGCTGGCCTCCGTGCGGGAATCCTCGCAGGGCCAGTTTGATCCCAGCTGATTTGTTTCATAGCTGCGGATGACTCCATCCACGGGAAAGTTGTAAACGAACTCGTTCTCCCTCAGAACAAGGTTAACCTGTGAAAATAGTTTACATTAATACAGATTCTTACTCTTTATGTGGGAATTACAAAGGTTCCCATGGTAATAGTTATTAATCAGTTAATTCTTATTCGGTTCCTAAAAATTTTGTTCTTAAGCCGCTTGGTATGGACTCTTGTCCAGTGCAAACGATCAAATGAAATTAACTGCTCAGTAAAGGCATTATAAATGGTTGCTGTAGTTGAAATATGAATATGGAAAATCGAATACAGTCGGGTCGACGTCGTTCA

CGGGACTTTCTTGAAAGCCCTTGAAAGTAGTTATTGGATCTTCAGAAAATTATGCAGGCTTTATAGTTAAATTTTTAAACGGCTTAGGGATGTAATGCATAGATTTGTGGTTTCTAAAAGTAGCTTCAATAGGAAAAATCCAAAATAATTAATAAGTAAAGAAGTACTTTCGATCGAAGATCTTACAAACTGATTACATTTTACGAACCCTGCCCAGCTTGCTAATGACACTTGCCAGGAACCCCAAATCTTACATCGTATGGACTCAATTGAGGCAGTTGCGGCAGCAGACGCAGTGCATTCAAACTGAACTCCCGCACATTGGATCGCACGTAGCTGGCGGCGTCGTTCAGCACGAACTTGAACATCTATATGGAGATTTTGTATATGCTCTCGCCCCGCTGTGGCTATAATTAACGGTAATGATATTGGTTCCGTAACCAAATCGGGGGAGGAGTAGGCAAAGAAGATGGAGGAGAACGTGGTGGATTCCGTATCCCGGAGCCTCCGGCTGATTCACCGACTCCGGTTGCCGGTTGCTGACAAAGGGATCTTATTTTCCGCTGTTGCTTTTGATTGGCCGGCGGCTTGTTTGTACATTTGTGTGCTGTGAAATACGAAGCGGACGGTGTTCACGGAAAACAAACGTAAAAAGAAAATACAGTGCGTACTAGCGATGGGTTTTTCTCTGCACGAGACAGCCTAGAACGAAAATGAACACGCACGTCTTAATTTATGTGTCAAAGCACTGTACAAGGCCAAATCTTTTTGTGTAATTATTGTAAATTTATTAGCTTAATATGAAAGATTATTTTATTTTTTTTGTCATATTTTCTCAGAACAATTTAAACTAACTACATTTGTATGCATTCATCGTGTCACGCGAAATCGCGTTCTTTGACCATCTCTATTGGGCGGCGGTGCCAGACTGGCGGTGTTGCCATTAGCCGGTTACCGTTTTCAAGCCAAGTCTAGCTAGTTTTTGTGTACCGCCGAAAAATACATCTAAGCTTGTTAAAAAATCTGAAAACTGCCAAATTGATGTGAGGACACTATTTTTATTAAATAATTTAGAAGATATAACTGTTTAATAATGGTAATGAAATAATTTCATTGTGGCTACTATCGATTACTTTTGCTGGTCTGGCAACGTTGTCATTATCACACACACGCGCACGCATATGAGAAAA

 

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