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CRIMIC CR01550

Gene Bsg25D

CG14025

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:5271728..5278548	25D2-25D3	FBti0220967	94941

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTGACACGGATTTGGAACCGTGTGG	GAAGCTCTGTGATTTGGATGTGAGCC

Images

Construct Design Sequences

sgRNA sequence	AACAGAGGGTGGCAGGGACAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGCGGTCATTAGTCATCCG	CCTGCCACCCTCTGTTGTT	GGGGATCGGCTAGCCAAAG	ATCCGGCGGTCAGTTAACAG

 

Amplicon 5'

Amplicon 3'

TCGCGGTCATTAGTCATCCGCAGCGTTATTTCACGCACCATGCTGGCGTGTCTTTGCTCCAGCTGCTGCACCTGCTTCTTGGAGTTATCCTCCAGCGACGCATGCCGCTCATCCACTTCCACGGCAAGCAAAGCCACCCTTTGGTTGGCCGCCTTATTATCGGCCCTCAGCTTGAGGTTCTCCTCATGCTGCTGCCTATAGGCAAGTCTGTAGTTGCCCAACTCGGTGGCCTGCAGAGCAGCCAGAGCGCGCAACATATTCGATTGCTCGTGATCCCCATCGATGCCCCGCAGCTCCTCCTCCAATGCCTTAGTTAGCTGCTGCAGGTTCACCTCCTCCTCCTCATCGAATCCCAGGACGTGAAGTAGGTTGCGAGTGTTGGGAATGCTGGCCAGCTCCCAGGCTTCCATAATGCTATGCACTGAGATCGTCTCCAATTGCTGCGGCGTCACGGTGTCTGTAAATCATGGCAATTAACAATTAGCGTGGGACGAATGAGATTGGCTTCGCTGTTGTATATACACATTTATTATACATATAGCCCAAGATACCAGGTGGGTGTGGGATTGGATTGGGATTGGGAGTGGGGAAGGGTGTGTGTGTGTGTCGATGGTGTAAGCAAAAGCGCCGAAAGTTTGTTATTTCTCTATATCAAATGGCTTTTCGAAATGAGAAATTTTTGGCGTGTTATTTTAAGAGGTAGAAACTAATAAATCAAGAAAATAGAACAAATTATAAGTAAATATCCTTTCTAACTGAAATTGATTTATATGAAATCCTTAATTAAATTGATTGTCAATGCATTACAGCATTACTAAGGGTTGAAAATGTAGGATATGTGATTATACGAGTAAATAGAACGAATCAAATGTGCGGCTACATAGGGTGCGATTAGATACCGAATGACTGGGTTGGGGATTCGGTGGGTGTGAGGGTGTTGACAATTGCTTCAGAGCGCACAAACCTTTGCAGCATGACCAACGGCCGCCGTGGTAGCATCTCCAGTGCTCGGACGACGGTGCGGGATTGGTGGCCAGGCGATGGCCTACGGTGTGTACCGACAAACAAGTGTTTTGCTTTTAACATTGCTTCAAACGTAATTTTGGCATTTTGGGCTAACAACAACAGAGGGTGGCAGG

GGGGATCGGCTAGCCAAAGCTGGTCTGGCCAGAGGAAACTTACCCAGTGATTCCACGCTGCTGCTGTGCTGCGCCTGGCTCTGGATGGAAAGCTGTTTCAACTTGGCCGCTGCCGTCGGTGATGTAGTGGCTCCCGTGGTGTTGCTGTTCATCTTCCGGCGTGCTGGCAGTGAAGCACAACGCTTCAGTTTGCTCCCGCTGGTGTGGACGGACCGCAGACGCCGGCTGCCAGGAAGATCGCTCTGGGACGAGGAACGTTGCACCTGTGTGGTTTGGAAAAATAAGATGCTTATGATGATCATGATGTATATTTAAGATAATTTGTTTGCTTTGGTAGTGCTACTTAGGCATGCTCTACTGTATCAGCAATAATAACGATTAAACAAAATCTGTTTACTATGCAATTTTGCATACATAACCAGAAGTTGTATCGCCACTTCGTACGTGACTTGACAAACGACACTCACCTGAACTCCCAGCTCATCGCATCCGTTGAGGCTTCGCTGATTTTGCTGCTGCTGCAACTGGTCCTCGTCCGTATTGTCCGAGTCCGTGACGGATAACTCGTAGATTCCCTGCTGTGGCCTAGACCGGCGACCGTATTTCTTGGTGCCCACGACGAGTTTGGGTGAAACCTCGCGATCGGAAGACTCCGGCGGACTCTCGATGTATGTGTTGTTTAGCGGCTCATCCGTAATCACCAGTGAACGCTCTGGAAGATTCAATAGATATAAGTTAGCAGAGAACTGGAAGAGTTGTATGGATCTTAGGTGGCTACCAAAACGAGCGGCTAGCTTCTCAATTATCGGATCTGTTAACTGACCGCCGGAT

 

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