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CRIMIC CR01554

Gene Ca-beta

CG42403

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:11165031..11165040	32D5	FBti0210430	86444

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GTGGATTCGGCTTTCATGCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACGGCAGGTTCGCAATATCA	CGAATCCACCAGCAGATCGG	GCTGGGATCTAGGGCTAGCT	AGGAACGTCAGGCCTTAAGC

 

Amplicon 5'

Amplicon 3'

ACGGCAGGTTCGCAATATCAGGTTGATTTGAATTTTCATCTCGTTTTTTTTTTGCTTTTTTCCTTTAAAACCGGTCTACGCACCATTTAATGCTGTTTAAAATGCCAACTCGAGGGTGTATGAAGTGTTCTGTTTGCTTTCAATTTACACAGATTTCTATAACTAAGTACGGGTTTCGGTTAGCAGGACATTACAAATTAGCCAAAAAACAGAGTTTTACGGACAGATGATACGAATTTTGGAATGAGTTTGTCTGATTGATTCAGTTTATAGAATATTTTACTTACGAAAATTGAAAAGATTCAGTTAAAAAGTTGAAATATAATAACTGATTAGATCTGTTCTGATAAAGTTGTAAAGTTAATTTAAGAATGTATTAAGCTTTATCAACTACAGCTGAGTAGCTAACTTTTTAAGAACTTTTTTGTTTTGAGTAGATTTGTTTTGTTTTGAAAATTCCTGAAACTCCCTGAAAGAGCTAGCGATATTTTTGCGATTGAAATGGATTTCTGAGATTCTGATGAGAGACGTGGACAAACCTTTGTTTTTCTTGCGCGGTGTTGTACTGTAGGGATCTTGGGTCGCGTTGTGGTTGTTCATTTGGTTAGTGTTGTCAACAGTTTTGTGTTAGATAAAAGAGAGTTTGAGTGCATTTAATAGTGTTCACATTGGCTAATTGGTTAGTTTACAAGCAGACCATGAACGAGGAGCTTTGATTTAGACAAACTAGGAACGGAGAAAATCGTGGCTATTGAAGCTGGTAAGGTGTGTACTTTTGTGTGTTTACAGTGGTGAATTTGGGTCTAAAACGGGAATATCAAGCAGTCTAGACCGATCTGCTGGTGGATTCG

GCTGGGATCTAGGGCTAGCTCTAGGCCGATAAATGGGCCGTTTGCATAACTAGGGACGGATTTGCATCGGATTGCCGGGTGAAGTCGTTGATATAAGTAAGCATTAACTTGCACTCGGTTTATATGAATGTCACATGATATATAATTAGATCAAACACAAAAGTACAAAATTACGGGAATTTAGCTTGGCCCACATTTTCTTTTTTTCTTTTTTTTTTCAATATGGTCTGGAATCTGATTTGGTTCGCTTTGAAATGAAAATCATAAAACACGCGTCCAATTACAATAGACATCGATGAGCTCGAGTCGGGCTCGGCATCCGTCTGTCGGACCTATGGATGCTCATAATCATATTGGGCTCCTCGAAGGGAGTACCTCTTTTGGTGATTTACAAAAGTAAACACATTACATAGAAGACAGCGAAAAATATATATAGATTGAAATTAATTCGATTCACAGAATTGAACAGATACGAAATAAAACCTCGCCCAAAAGAAATCAGGAGAATGAAATGTGTGTCATACCAGGTGTGGGGGGTGTGCTACCTCGAGATGGCTCCGCACCTGCTTGTCCGCCCGCACCAAGATTCCCGCTCGACGAGCCCTTTGTGCCATACAGTCTTGAGGGTCTGGTTTGGTTCTACGATAGAGAGGTCGTTATAGATCACACTTGGAAGATGTGTGAATTGGTTCTATCATATCCAACCTGCATGCGAATGTTGTCTAGCTTAGCGGGGCTGGGTATGAAGCCCACATCACAGCCCTCCTTGACCAGCCGGCCGATCCACCAGTTATTGTCGTACTTCTCTTTGATGTGCAGGAATTCCCGGATCTCGAACGAAACTGCTCCGCCCTGCACCGGCGAATCGTCGTCGATGGCGCCATCGTATGCTACGTTGGTTCGCACAGCGAACGCCACTGGTTTGCTCTGAAATTACAGGGTATTACAGGGTTGGATTTCCATTTTTATAATGCACAACACACTTACCCTCGCTTTATCCAACTGGCTTAAGGCCTGACGTTCCT

 

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