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CRIMIC CR01555

Gene Mtp

CG9342

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:20922587..20922608	38F5	FBti0210431	86445

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TCTTTGTGGTAGGTAGCGTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGATGACCTTGTCAGGCTGT	ATTGACAGTGCCACACCTCC	GCTACCTACCACAAAGAGTTCA	GGTCCAGTCGCCAATCCTTA

 

Amplicon 5'

Amplicon 3'

CGATGACCTTGTCAGGCTGTCCCCTAACCAGACTAATATAAAAAGGTCTATCGGGTATCCGGGTAATTGAGCGGGCTTTTCCAGAGGCGTCGACCTTGCTTCCGCTGATAAAAACCTCCAGTAACTGGTCCTCATCTCCACTCCAAACTGAGTTGATCTTGAGATCTGTCTCAAATGTATAGCTCGTCTCCGCGGAGCTTGAATCTCGTCCCAGTTCCTGGAGTATCACTTGATTCTGTAGCTTGAAAATTTGCTGACTGTTCGGAGCTATTAGGGCCGTTTTTCCATCCTCTACAGAAATGTGGCCGAAAAGAAATAGTAGAACACGTCATTAGTCAAACGAATTGTTATAACTTTCACCCAGAAGCAGCGCTACATGCTGATATGTATTTTCGTCGTTTACTAAGCCTGGGTCGAGGTTATCAACAAGTGGCCCTGCACAGTGGGCAGCGATAAGGCAAATAGTGGAAGCAACGTGTACGAGGGAGTGATAATCTGAAGGAGGGATGGGGTGATAGATAGCAAAGGTCGATGTGGATGTGTTTAAATCCGGGGATCTTTGGGAACAAATATGATGATGTTTATCTATGTTTCGGTTTTTAACAATTCAGGGTCGGGTAACTGCACGTTTTTGTCTAACTTTACAGAAACAAAACGAAACACCTGTGCAGGTCACATTTCCTTGACCTTCAATACTGTTACTTGCCCCACTGTAAAAACAGCTGCTTCAATTGGAAAGTGTGTCAGATAAAAGTTCTCTTTCGGCCTTTTGTTTGTGTGCGTAGGCGGAGGTGTGGCACTGTCAAT

GCTACCTACCACAAAGAGTTCATTCAACTTGATCAAAACCCATTTACCCAACTAATCGCAAATATTGGCATTACTTACCCAAAAGTCCCAAAAAGAGGGCCAGCAGTAGCAGGGTTCGCAGGCACTTCTTATTTTTGTTCTCCATTTTCCCAAGGTCTTTGTTCGATTCTGTACTTATATTAAACTGAGCAGAGGTAAATTAAACTTAATTCATTGATAATACCGAGCCGAGCCGGCAGGCGACGGAATTAAAAGCGGAATGAAAACAGAACGGAGCTGAGGAGCGAGATTCTGACTTGTGGTGACTTTAAGCAGCTGAGAGTCGCTGAGCTGGGCTGTGGTGAGATTGAACAGCTGGCGAGCAAGAGGGCGCCACTGTTACGGAGAGTGCCAGAATGTATGTAAATTATTCGAGCTTCTACAAACTTTTTTATTTTATTTAAATGAGTATACATGTTATTATAACCTCCAAAAATATTCATACTATATTCATGTAACAGGCACAAGGAAGCGTTTCCGCGCATATTAAGTATATACATTCTTGATCAGGATCAATAGTCGAGTCGATCTGGCCATGTCCGTCTGTCTGTCCGTCCGTTTGAACGTTGAGAAGCCAGGAACTTTAAAAGTTAAAAAGTTGAGATTAGAAACAAAGATTCTAGGGACATACAGGCAGCGCAGTTTGTTGATCCATGTCCCACGCACACTCTAACGCCCACAACGTTTCACGTTTTTGAACCGTTTTTCGGTATTTTTTCATAATGGTATTAGTCTTATAAATGTCTATCGATTTGCAAAAAACTTTTTGCCACGCCCACTCTAACGCCCTAAAGTCGCCAAACCTTGCACGTCCACACTTTAGAACAATTTTTTTTATTTATTTTATTACCCAATATCTACCGATATCCCAGAAAAATTATGAAACTTCCGCGTTTGCATTCACACTAGCTGAGTAACGGTTACCTGATAGTCGGGGAACATAGACTATAGCATTATCTCTTGTTTTTAATCTGATGTAGCTTAAGTATGACTGTAAATGTCTAAAGTAAGGCTGAGTTAATAAACGCTTAAAACAAATGTATGTATGTTCATCTTTGGATATACATATTTGCTTTTGGGGAACAACTACGGATATGGTTATCTTTCCACGGGGTTCTATTTTTTATATTTTTAACTAAGGATTGGCGACTGGACC

 

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