CR01557

CRIMIC CR01557

Gene bib

CG4722

Information

Donor Location Cytology FBti Stock number
pM37-p12L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK GATGGCAATTGTTGGATGTCAGCTGG GTGTTCGTCCTAGGTTACCTGCACAT

Images

Construct Design Sequences

sgRNA sequence TTCCGTGTGCTGGCTGGTTATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TCCAAGTGTTCTCCAGACGC TATGGTCATGGGGCTCCCTT GCACACGGAACCCCCTTTTT TGCACGATGTCCTTCGCATA
 
Amplicon 5' Amplicon 3'
TCCAAGTGTTCTCCAGACGCACATCATCAAGATAATCGCCAAACTAAACACGGCCAAAGTTTCGAGCACTTTGTGGCCCATACAAGGACCTTTTGCTCAGGGAACTGGCAAATAACAAACATTTCATCACGAGCTGATGGAGTAATTAAATCTTAGCCAGCAGCATGTCGAGCGCTTAAATTCCGGCTAAACTGCGGGACCCTTTTGTCTCAAGTCCTCCGACTGACCATTTAAAATGAAAAACTTGAACTTAGTGGGGAGCAGAGAACTTAGGCAGAAGTTGAGATACATATTTAGTTCCAATTAAAGTGAAGTGCCGCTGCCAAATTAAACAGCTGAATACATGATTTTATGGAAATTCATAAAGCCCTCGATTTGTTTGCATTTTCGTTATCGTGCAGCGGACTCTGCGCTTTTTAGCTTAATGTGAATCAACTGAATTCGGCAGCATTGCAGCTGCTCATCAGAACGCTTCCCCTAGAGCTCCCCCATCCGCTCCAGAACCCACTCGCAGTCCCTCCAGCTCCTCCTCAGCCTGCCCACCCGCCCCCTGCTTAATTGTCACTCCACGACTTGAGGGCAACAAAAAAGAGCTTTGTGTGCAACTTGATTTGTGCCGTACAGTAGATACCAGCTAACTTGGAAAAGTCTAGGGCTGCACGACAAAAACTTTGAAACGGATTTCATTTGATTTCAGTATGAATCCTTTGGCTCAACACGCTTAGCCTTACTATACTTAAGCGCTGAAATATATGTTTATGCAACCAATATTCACTGTACTGTACACATTCCACATGCAGTCGGTGGAGCCACATGCAAACCAAACCCAGTTGCGGAACCATGCTAACCCCGGGCTCGTTCGCCTTCTTCCATTCCTCTTCCATTCCAGATCCATCATCAGCGAGTGTCTGGCCTCCTTCATGTACGTGTTCATCGTCTGCGGTGCCGCCGCTGGCGTCGGAGTGGGGGCCAGTGTGTCGTCCGTGCTTTTGGCCACGGCTCTGGCCTCCGGCCTGGCGATGGCTACACTGACGCAGTGCTTCCTCCACATCTCGGGTAAGTCGAAGGAGACAATAAACAAGCGCAAAAACATCAACAGAATGGGGCGACAAGTGTCTCTTTTCGGGTTGCCCATTGGAATTGCGTGGGAAACTGCCGTTTTCAAGGGAGCCCCATGACCATA GCACACGGAACCCCCTTTTTTTTTGTTTGCTATACTTTCGAGAAGGCAATGCACTCGGTGCGGCTCCACCTACTCCAGCTCCTTGGAATCTTATCGCCCCCGGGCCAAGTTCCACCACTTCTTTCTTTTTCCCTTATTCCCTTGCATTTATTCTGGTATTTATGTATTTGTTCATTTGCGTTGACATTCAAGGCAATTAAATGTCTTTACAAGTAAAAAAAGAAAAAGAGAATGAAAACAGACCAAAGACCAAAGTAATGTAGTTGGGACCCCATCGTGAAAAGCGTCTTGAAACATGTGTTCTTGATTTTCCTGCAACTCCCCCATTTTGTGTTGGCTTGCAATTCCCCGCCTTTTCACAGCCAATTGGTTGTGCATTAAGTTATGATGTTGTTCTATACGAAATGCCTTCCGAAGAAGTGTCAAATTGATATTAAATGTTGCTCCCCTTTGGGTGGCCACCACCAGAGATTGACGTCAGTATCTAAAGATATGCTTTCCCCTTCATAGGCGCCCACATCAATCCCGCCGTAACCCTGGCTCTATGCGTAGTGCGATCCATATCTCCGATCCGGGCTGCCATGTACATAACCGCACAATGTGGCGGAGGAATCGCCGGAGCTGCTCTGCTTTATGGGTAAGTTTTTTCGTATTTATTAAGTTACACATATCTTAAGTAGACAAATCATTGGTTTGAAAACAAGTAAGCCAAAATCATAAATAAACATTGCCCAATAAATGGAGTTTAAAGCGATATCCTTTTGAAAAGCCTCACATGAATAATTTAACCAATTGATTGAGCAAAGCCATTGATAGTCGAGGCTATCTATTATGCACATCAAATCCCCGCATTAAAAGCCTTATGTTGTCGCATACAGCACGCCAAACTCAATTGAACAGGAATTCATTTTAAATTTTCGGTGTGCAAGAGTGTATTTAAATCTGTGTATATTTGCACGCCTGACCACAGCAATTGCTGCATTATGCGAAGGACATCGTGCA
 

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