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CRIMIC CR01557

Gene bib

CG4722

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:9984645..9995538	30F5-30F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATGGCAATTGTTGGATGTCAGCTGG	GTGTTCGTCCTAGGTTACCTGCACAT

Images

Construct Design Sequences

sgRNA sequence	TTCCGTGTGCTGGCTGGTTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCAAGTGTTCTCCAGACGC	TATGGTCATGGGGCTCCCTT	GCACACGGAACCCCCTTTTT	TGCACGATGTCCTTCGCATA

 

Amplicon 5'

Amplicon 3'

TCCAAGTGTTCTCCAGACGCACATCATCAAGATAATCGCCAAACTAAACACGGCCAAAGTTTCGAGCACTTTGTGGCCCATACAAGGACCTTTTGCTCAGGGAACTGGCAAATAACAAACATTTCATCACGAGCTGATGGAGTAATTAAATCTTAGCCAGCAGCATGTCGAGCGCTTAAATTCCGGCTAAACTGCGGGACCCTTTTGTCTCAAGTCCTCCGACTGACCATTTAAAATGAAAAACTTGAACTTAGTGGGGAGCAGAGAACTTAGGCAGAAGTTGAGATACATATTTAGTTCCAATTAAAGTGAAGTGCCGCTGCCAAATTAAACAGCTGAATACATGATTTTATGGAAATTCATAAAGCCCTCGATTTGTTTGCATTTTCGTTATCGTGCAGCGGACTCTGCGCTTTTTAGCTTAATGTGAATCAACTGAATTCGGCAGCATTGCAGCTGCTCATCAGAACGCTTCCCCTAGAGCTCCCCCATCCGCTCCAGAACCCACTCGCAGTCCCTCCAGCTCCTCCTCAGCCTGCCCACCCGCCCCCTGCTTAATTGTCACTCCACGACTTGAGGGCAACAAAAAAGAGCTTTGTGTGCAACTTGATTTGTGCCGTACAGTAGATACCAGCTAACTTGGAAAAGTCTAGGGCTGCACGACAAAAACTTTGAAACGGATTTCATTTGATTTCAGTATGAATCCTTTGGCTCAACACGCTTAGCCTTACTATACTTAAGCGCTGAAATATATGTTTATGCAACCAATATTCACTGTACTGTACACATTCCACATGCAGTCGGTGGAGCCACATGCAAACCAAACCCAGTTGCGGAACCATGCTAACCCCGGGCTCGTTCGCCTTCTTCCATTCCTCTTCCATTCCAGATCCATCATCAGCGAGTGTCTGGCCTCCTTCATGTACGTGTTCATCGTCTGCGGTGCCGCCGCTGGCGTCGGAGTGGGGGCCAGTGTGTCGTCCGTGCTTTTGGCCACGGCTCTGGCCTCCGGCCTGGCGATGGCTACACTGACGCAGTGCTTCCTCCACATCTCGGGTAAGTCGAAGGAGACAATAAACAAGCGCAAAAACATCAACAGAATGGGGCGACAAGTGTCTCTTTTCGGGTTGCCCATTGGAATTGCGTGGGAAACTGCCGTTTTCAAGGGAGCCCCATGACCATA

GCACACGGAACCCCCTTTTTTTTTGTTTGCTATACTTTCGAGAAGGCAATGCACTCGGTGCGGCTCCACCTACTCCAGCTCCTTGGAATCTTATCGCCCCCGGGCCAAGTTCCACCACTTCTTTCTTTTTCCCTTATTCCCTTGCATTTATTCTGGTATTTATGTATTTGTTCATTTGCGTTGACATTCAAGGCAATTAAATGTCTTTACAAGTAAAAAAAGAAAAAGAGAATGAAAACAGACCAAAGACCAAAGTAATGTAGTTGGGACCCCATCGTGAAAAGCGTCTTGAAACATGTGTTCTTGATTTTCCTGCAACTCCCCCATTTTGTGTTGGCTTGCAATTCCCCGCCTTTTCACAGCCAATTGGTTGTGCATTAAGTTATGATGTTGTTCTATACGAAATGCCTTCCGAAGAAGTGTCAAATTGATATTAAATGTTGCTCCCCTTTGGGTGGCCACCACCAGAGATTGACGTCAGTATCTAAAGATATGCTTTCCCCTTCATAGGCGCCCACATCAATCCCGCCGTAACCCTGGCTCTATGCGTAGTGCGATCCATATCTCCGATCCGGGCTGCCATGTACATAACCGCACAATGTGGCGGAGGAATCGCCGGAGCTGCTCTGCTTTATGGGTAAGTTTTTTCGTATTTATTAAGTTACACATATCTTAAGTAGACAAATCATTGGTTTGAAAACAAGTAAGCCAAAATCATAAATAAACATTGCCCAATAAATGGAGTTTAAAGCGATATCCTTTTGAAAAGCCTCACATGAATAATTTAACCAATTGATTGAGCAAAGCCATTGATAGTCGAGGCTATCTATTATGCACATCAAATCCCCGCATTAAAAGCCTTATGTTGTCGCATACAGCACGCCAAACTCAATTGAACAGGAATTCATTTTAAATTTTCGGTGTGCAAGAGTGTATTTAAATCTGTGTATATTTGCACGCCTGACCACAGCAATTGCTGCATTATGCGAAGGACATCGTGCA

 

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