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CRIMIC CR01558

Gene Fmo-2

CG3174

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:6643636..6643647	42B2	FBti0210433	86447

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AAACTTAGACAGTCTCTGTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTTGCCGCAGTCCTTCATT	GAGACTGTCTAAGTTTCCCCACT	AACACGCTAGTCAACGATCA	TTCCAAAGGAGGTCATGGGC

 

Amplicon 5'

Amplicon 3'

TCTTGCCGCAGTCCTTCATTAGCTTGTGGATAACTGGCTTGATGTTCTCGATGCCCGCGATACTGGCCAGTTCATTGTAGTAGACAAACTGAAATTACAGATAAAAGATTGTTTTATATATGTTCTCTGCTTGTAAAAGTCTTTAACAAATAAGTCTACATAAGCTAATATTTTTAATTTCTTATACAGGGTATTGTGAACACTCACCTGTCGCTCGCCCATCTGATGTGCTTTCTTCTGGTTGCGCACGCCGCAGCCCCACCGCTCTCCGATCTCCTGCTCCAGATCGGCAATCATCTGCTCCCGGGAGGGAAACTTCCGCTGACCTGTGAAGAACTTCAGCGTGAAGCGCACCTGCATGTCGAAGATGTGGGTGGGAATCACGTTGAAGGGCAGGCCCACAAAGGCCATGGTCGGGTGGTTGATGTTGATGCAGTGCTTCCACAGTGGCTGCACGAAGTTGTCTATCACCTGGACACCCACATCGGTGCTGAGGCAGGGGAATGTGTACTTGTAGCCGGTGCAGAACATCACGTGGTCAAAGCTCTCCGTGCTGCCGTCCGTGAAGACGGCTCCATCCTTTGTGAAGCGTTTCACATCCGGCTTCTGCGTTACGTTGCCCATGAATGCTGTGTTAGGGGTCGTGGAAAGGTGGTGGCTTAGGAAGACCTGCTTGGCAGCCAAGCGAACATGGTTGGTGATGTCCATGCCACTGGGGCCGGCGCCAATGATCAGCACACGCGCGTCTGTAAGAAAAACAATTTAGCCGGTAAATGTTTTGGTGCCAAATAGGTTTTTGCGCAGATAAATTAAATGGAAGTGGGGAAACTTAGACAGTCTC

AACACGCTAGTCAACGATCATGTAACATATATCATGGATGGCTCATGTAGTAAAGCTTCTATCATTTTATAAAACAAAAGTTTTATGTGGATATATAAACCTTTCAGGATTTAAAAGATCACCGAATCCTAACCGTGCAATGCAACACCAAATGCAGTATTCTATTCTATATCAACAGGTTTAAGATTTTAGTTCAGTTGTTACTATAAAATTTCTGATATATATACACTGCTCTTAAAAACTACAATGGTTGGCATCTTTCCAAAGGACTCTCCCGATCTCCGTCTTACTTTTCTATCTACAATCTGATAAGTAGCGTTTTGAGATCTCCCACAGTGCCAGCTATCAGCAGTTAAGCACGTGCTAGAGGAGCTCGAAATTGGCACAGACCCACTACACTTATTATAATTATGTCACACAGCCGTCAACAACATTATCTGCAGTCGAGGGCCGAGTTGTTTATGGGGAAAACAAGAGTGGGACTCCGTCCAGACGGCGATGGAGGCGAAGTCCGAAGCTTTGGCCTGACTGATTTAAGATACAGACGGGAGCGCTCTAATCTCACCTTTAAACTTATCTGCCTTGCGGTAGAGGTGGCTGTGCATCTTGTTGCCCTCGAACAGATCTAATCCCTCGACTTCCGGGAGGTCCGGTTCCGTGTAGTGCCCGTTGCAGACATAAACGAAGTCGTAGTAGACTGGGTCACAGGTATCCGTGCTGTGGTCCCATACGTAGACCTGGAAGTGATCGGAGAAGGCCCCGTGAGATCGATTAGTATTTCGCCAGTCGAACAAACAAGTCACAGTGATGGAAAACTGGTTCCAAAATACGTGACGTCGCTACCAACCTCCCAGTCGTCCAAGCGAGGACGCACTCTTATCACCTCGTGCTGGAGCTTGATGTGGGCCTTTAGCTTGAAGTGTTCCGCGTAGGAGCGCAGAAATTCTAGCACCTGGTTGGAGGTGATGAAAGACTCGGTGATATCGTCGGGGTACGAGTAGTCCGGATAGCCCATGACCTCCTTTGGAA

 

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