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CRIMIC CR01565

Gene PIP5K59B

CG3682

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:22875239..22875252	59B4	FBti0210435	86449

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTCATGTCCATCGCATCCTTAGCTC	TCCATGAAGTCGAGGTCCTTGTACGT

Images

Construct Design Sequences

sgRNA sequence	CCACCCCCGCACTTGTCAAACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGCATTAAACCAACCGCCT	GGTGGATTCACACTCACGCT	AAACGGACTTCCATCGGCTC	CTCGTCTGCAGGCAGTACAA

 

Amplicon 5'

Amplicon 3'

GTGCATTAAACCAACCGCCTTCGTCGTCGTTTTTCTTTGCTAAAAAACTAAACAAATTTTAAAATTGTATTTTTATTGTAAAATTACGCTGTAGATTGGACTCAAGTCACACATATAATAAAAAAAAGTCTCAGCATGACTAATTAAATCCAAATTACTTTTGTTTATGGCATCTATGGATTGGTTTAAATGAAGTTTGTTTTTAATAATTCGTTTTAATCATGTGGGCGTTACAGGGCCACAGGGTGTTATAAAATTTATTAACATAAATTATTATTTACTATTTTTATGTTTGTATATTATATTTCCACGCGTCAAAGGCTTATAAGCTTTAAAAACATTGCTATTTGCGGATTTTACAACAGTGCGGATGCTCCGCTTCACCGTCTGACTGTGAATGCAACCCTGCGTGCCTGTTCCTGCGCACACCCTTCCAGCCCTGCCGACCTTCCAGCCTTCCAGTGTGCCTATGAGTATGCAGTGAAAATGACAGCAGTGGCAGAGGAGCCATCAACATCACCATCACCGTAGACAGCAGAAACGTTGACGCCAGCGCCAGACGAGAGCCAAGGTTCCTGCCACCCAGTTAGCCCACTCGATTGGATAATCGCCGAGGATCGGAAAACGTGAAAGCAGCCCTCGAATAGCCCAAGTCCACAATCCCAGCCAGCCAGCATGTCCTAGTGAAGTCCTAGACGTGGGAAGAGTGCAGAGAAGAGAAGAGAATACGATAGCAGAGCAGGAGCTGCACGATGGCCTCCGGCGATGGCGACACCATCAACACCATCGACATGGACAGCTCCTCGGCGTCTCAGGCCAAACTGGCCGAGCCCAGCAATGGTAACTATTATCCACAGACCCCGTCACTTTTCGCAGCGTGAAAAAAATCAATGACTGTCGAATGGAAATGCCCCTGGCGCATGTGTCTCGCGCGAAGGGAGGAAGCGTGAGTGTGAATCCACC

AAACGGACTTCCATCGGCTCCTATTTATATTTTGGGATTGATAACAAAAGAAAGTCACACGCGACTGGAGGGAGAGTGTCCTGTCCAAGTTCAAGTTGCGGTGATTGACCAGGCGACTGCGTCATGGCCTGAATTAAACATGACTCCTAATAATGAACTCTGTCCGTCTTTCTTTAGCCTCCACCGACCATGTGGGAAACTCATCGCCCGACGTAAGTACATCCGCCTTGACCTCGAGGTCACTCCCATCCAAACTAATCGGGTCAATTACTTTCACTCCCAACCAACCAACTAACCAACCATTCATCCGTCCAGCTGGGCAACCGACCGAACCGAGCGTCCAGCAAGGCGGACAAGGAGCGCAAGATTGGCCACAGACGCGTCGGCGAGGGCGGCGAGATTACGTACAAAAAGATCCAGACGTCGCAGATCATGGGCTCCATCCAACTGGGAATCCAGCACACTGTAAGAAACATTCATAGCCTTAAGTACTGGAGCTTGCATGTTTTACTAGCACTATTTCCAAACATTTCAATGAACTCAGGGCGGGGTTACAAGATTGACCCAAGCTTGATTCGTATGACATATCTTGTATATCTTGGCTTTTTCCATCTACTTAGTAATCTTGAAATATCCTTCGCAGGTCGGCAGTCTGGCCTCGAAGCCCAAGCGTGATCTACTTATGATGGACTTCTGGGAAATCGAGAGCATCACCTTTCCACCAGAGGGTTCTAGCCTTACACCTGCCCACCATTACAGCGAGTTCAGATACAAGATCTATGCGCCCATAGCGTTTCGCTACTTTCGGGATCTGTTTGGCATCCAACCAGATGATTTTATGGTGAGTGGGCGACCGTTCCAAGTCGGCAGATACTGATGGTCCATACTTTCAGATGTCCATGTGCACGTCCCCGCTGCGGGAACTGTCCAATCCTGGTGCTTCCGGCTCTATATTCTACCTGACGACCGACGACGAGTTCATCATAAAGACGGTGCAACACAAGGAGGGTGAATTCTTACAGAAACTACTGCCTGGGTGGGTAACCCGAACAAATCTTTGCAAGAAGACCTATTTAAAAAGTGTTATCTGCATTTCAGTTACTATATGAATCTAAATCAAAATCCGCGCACGCTATTGCCAAAGTTCTTCGGATTGTACTGCCTGCAGACGAG

 

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