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CRIMIC CR01566

Gene CG17985

CG17985

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:7651625..7655302	43E7-43E7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGATCCGAATGTGATCGGAGTTC	GGATCGAAGCCATCCAGTTGGTTA

Images

Construct Design Sequences

sgRNA sequence	CCCAGTGTTAAAGTCAGTGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCAGCTCAGAGCCAATGAC	GATTCGTCGATAGCGCTAGC	TGGGAACTATCGATGTGTAGCC	AAGCGCCTGAACAAGATCGA

 

Amplicon 5'

Amplicon 3'

ACCAGCTCAGAGCCAATGACGCGGATAAAGCAAGCGTCCGTGCGGTTGGCAACGGCGCGGGCACACAGGGTCTTTCCCGTTCCCGGTGGGCCAAACAGTAGCACACCTTTGGGCGGCTCAATACCCAGATTGACGAACTTTTCCGGGTGCAGCAGTGGCGTCTCCACCACCTCGCGCAGCTTTTCGATTTGTTCCTTGCAGCCGCCCACATCGCTGTAGGTGACGTCCGGCTTGTCCTCAACCTGCATCATGGTAACCGTAGGATCAATCTTGGGAGGCAGCGGAATGTGGATCTGGTACTTGTTGCGGTCGACACCCACACGCATTCCCTCTTCTATGTCAGTAGGGGCCACCGAGTCAGCTAGGTCCACCACGAACTTGGCGAACTGCTTAACATTGATGATATACTTGGGGTCGTCGGAATCCGCGTTGATGATCTTGGTGCATCGGGCAACCTGCAGCGGTTGCTCGTTTTGCAGGATCTGCTTGTCGGCGGCCAAATCCCAGAGCGCTGGTGGCGCCAGACCCGTGTCGCTTTCCTTGATTCCAGTCAGCTCGTTCACCTGCTTCACAGCCTTTTGAATGTCCTCCTCGATGCTCTTGATGGATTTGTGATACTGGCTCTGCCCATAAGTCTTTAGAAGCTCAATGTCGCCTTCGTCGAGGGACTTGATCTCCTTGTCCTCCTTCTCATCGTGCTTCACCTTGCGCTGGTCGTCGCCCAGGTAGTCCGGCATCTTATTCGATTGCAATTGTACGGTTTTTAAGGCAAAAAATGAGTTTATGCGATGTTTTCCTTTTGTTTTACGGCGAGAATGACTGAACAATTTGGCATAGAGATGGGAGAAGCACCGCTAGCGCTATCGACGAATC

TGGGAACTATCGATGTGTAGCCACTTTTAACTTCTACTGTTAAAATACAAATCTTAAACTTTTCCCAAAATTATTTTATGGTATTTCAATTGGAAATATACTAATTAGTAACTGGAGCTTAAATTTTTAACACATAAAATGTCTAAATGCTGTTATATTTCTTATCTTATCTTAGAGCATGTTGCGAATTTCCATGCATGTGTTTTAGTTCAAAATTGGTAGATATTTGAATTGAAATAGATAAGAAACTTTGTTATTTTTAAAGTACTGATATTGCTTGATTGATAAGTAAACATACATTCTGCACCCTTTACCTTACCTGTACTTTTATTTTTAAATTAAATAATATATATGTACACTCGCACTGAAATCAGCACTGACCTTTCTTCACCAACAGTGAAAGTATTTCAGAAATTTTTAAAACCGCGGCGAACTGTGGGCTTTCATTCCCAACTGCATCTTCTGTACTCACCTTCGTCATTCGGCGCACCCTGGGCTCCCTCGTAGAATTGTCCAGCAGCTCTGCTCGTCGATGGTTTCTCGTGATCCACGGCGCCTGAAGCATTTACCGCCGCCACCAGGAGTCGCTCATCCAGCATTTGCCGCGCATCCTCGAGGGGATTGCGTTCGGGAGCTGGTTCCCGCTCAATATTGTGTCTAGGACTGTTGTTCCCGCTGCGGTGGACCGCAGGAAGGGCTTCGAGGCCCCCGTTGCCCAGCAGGACGTTGTGCACTGTAACCGGAATGCGAATAACCCGATGTGCGTGGATTTCATTTTCGCGGTCGATCTTGTTCAGGCGCTT

 

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