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CRIMIC CR01569

Gene Coq9

CG30493

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:7700188..7700197	43E11	FBti0212932	91260

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	GTTTCGACTCAGTGTGACCAGAGTTG	CGTTTCCAAGCTGGTAATGGAACTGG

Images

Construct Design Sequences

sgRNA sequence	TCTGGCTCACTACATCACCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGATTACGCCGCTTTCCAAC	AGTGAGCCAGAAGGTCCTCC	GTGGGACTCTTTCGGGAGC	TAAACTTCCGGCATGGGACC

 

Amplicon 5'

Amplicon 3'

GGATTACGCCGCTTTCCAACACAACGTAAGTGGCTTCCTGGTGAATGCCAAACAAACCGATTTCAATTGCCTCAACGACTTCAAGCCGCTTAAATGGCAAACGTGCGCTGTTTATACAAGCTTATTCGTCTGCAGAAGCTCCTGCTGCCGCTCAGTAAGTAGTGTGAACTCCCCAATTCACCTGTTAGCCGCGAATTCACCGCGTGTGCTCATTACGAAAGAGAGTCCGCAACCGACAATCAGCTGTTCGATCGAGTTGTGCGTGCGTGCGAAAGAAAGGGGGGAGAGAGAGAGAGAGAGAGCTAGTAAAGGGAACTGCTGACTGTCAAATGCCGGCGGCCTTAGATTTTTCGACTATTTTCAATAAAATTGTGAAATACCAGTGAAAAATTAAAGCAAATAAATAAAATGCAGGAGCAGGAGATGGAGGTGGAAGTGGGGGACCCGGCAAAGGCGTCGAATTTGCTGCGGCTCATCAAGCAGCTGCTGCTGGAAAAAGCTTACGATGGCGTGCGGATGTTGTTTCAAAGTGCCCAGGAATCGGAAAAGAACACTCGGCTGCTGCCCCACATAGCCATGGATCTATACCATGACGTGTGCTTGGAAAACATTTCCGACGAGGTGAACTCCCAGGAGCCAGAACTATTCGACTGCTCTGACGAGCTGCTCAAGCTGCTGGCCAAGTACGCCCCACTGCATGAGCTTATGCTGGAACTGATGGAGCGCTTGGAAGAGAGTAGCAGTATCAATGTGTTCGGCGCCTATCTGCGGGCCCTACAAGTTGTGCTGCAGCGACAGGGACGCGACAAGCCGCAGGCCGTTGAGTGGAGCCTGAATAGCATCTTCACCCATCTCAAGGATTTGCCCCTGCCCGAATACCTCTCCGAGGGCTATGACGAGGCCCAAGCGCGACTCATAGAGCAAAACGAACAGGTGGAGGACCTTCTGGCTCACT

GTGGGACTCTTTCGGGAGCCGCTCCGCGATGAAATTCTGCAGCAGGATGTGCGGACACCCAGCCAAATATTCAGGGATTGCGGCGTCAACCGGCGCAACATATTCACTTGCTTCTTCATTCAGCTGTTGGGACGTCCGTTGGCCCTGCTGGAGATGAATCACGCCAAGGAGGACCTTACGAGATCCTACGTGCAGCAGGTTACGGAAAGCCTGACTCAGGATGCCAACCAGTTCCTGGGGGATCCCTTCTACCTGCTCAACCTCGTGGAACAGCGTGCCAGATGGCTAAGGAAACTAGATGCCTCGAGAGGCGTCTACGAGATGAGTTGCCAGAATGTTTTCCTCATCGAGGAGAAACTTCCGCTGCATGCTGTAGCCATGTACTATCACTCGCTGTTCGTGGGTAACCAACTGCCCACAAACGCACCAAAGGTATATGCACCTTTATTCCTATTCGAAACGATTGTCTATCTGGCTGAAGTGCTACTCAGGCAGCAGGAGCCTCCCCTACAATATTGTGGTCTTCGATTAGTGGAACATCATTTGAGTACCTTGCAGCACTCAGTTCCCACCAGCTCCCTCCAGCTGGATGTCCACAAGCGCTTTTGCGAAGCCCTTTGCAAGATTGTGGGCTATTCGCCGCAGGTCGCACTTCGGCAGTTGGGAATTCATGTGCTCAGGCAATTTGTTCTGGCCTTCGATGATCATGGAAAGTATCTCATCCTTAAAAATCTGATGGGGACATTGCAGCACGGCGGACTAATGGGCTACCTCGGAGGGATGTACAAGGACCTTGTTGACAAGGCGCTAGGGCAATCTGGTCCCATGCCGGAAGTTTA

 

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