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CRIMIC CR01572

Gene CG32109

CG32109

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:12745518..12745528	69E1	FBti0210436	86450

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATACGAGAAAGATCCACGCAACCGA	TCAAACGAAAAGCGACGATCGAAGGC

Images

Construct Design Sequences

sgRNA sequence	AATACTGGGACAACAAAAATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCAGGAGCAGAAGTACGTT	AATAGGGCCTTTGAAGAACACT	CCAGTATTTTCACGTTGTTGCAG	CGTAGATTTTGCGCCGTGAG

 

Amplicon 5'

Amplicon 3'

GCCAGGAGCAGAAGTACGTTACTTGGATGCCGGCCATGTGAGCGCCTACGTTCTGCATCAGAAGCTATTTAGGTGGGCATAAAGTTTTCTTTATAAATTTAAATAAAATTATAATAGATCACTTTTCTTTTCAGATCCTGCATTATTGAGGCTTTCGAGCGGGCAAAGAAAATCTATGAGAATGACGTGGGCGAGGGCCTGAAATGTGATATAACCTATGAGCAGTTGATAAAGAGATATGACAAAAATATTCAGTAAATATAAGTTAGCATATCAAAACATAATGATGAATATGTTGCCATTTAAAGAAAAGTCTAGTAAATTGTACATAATTAGGTATTTTACTTGGGAAGGAGTACAGTTTTCTTGATTTGGAATGGAAACTGGTTCCCCATATATTTTCTAATTTATATTACCACGCCCACCAAAACGACCACAATCGACAATAAATATAAACGCCAGTGAGCTAGTGAGCTATCTAAAAAAATAGTCTTTTCTTCTTTCGTACCAGCTGAGTAAGGGGTATCCTATAGCCGGGAAACTGAAATAAAGCGTTCCCTCCTTTTTAATATATATGTAAATTATTAAAAAAAAAAAAACATTTTAAAAAAGTGCCGGTAAAAGCAAAGCTTTATTCATTTGGAGTTCAGAACTTTTCGTTCTCGAAGCTTGGTCACACTGGTGCATAGCTTTTGTTTTTGTTGACTGAAATAAATAAAGAAATTGCAATATAATGGATCCAGTAGTACTTGTTTTATCTTATGGAGCAGTTCCTGCACTGGAAATAGTCGAAAGTAAGATTAGTTACTTGAAAAAGTACAGAGTGTTCTTCAAAGGCCCTATT

CCAGTATTTTCACGTTGTTGCAGCTTTGTTTATTTGAAATTTATGAGTAATACAGGCGAATTAAATGTTTTTATTCTGTTGGTATGAATACAGCATACTAATCATACAATGCATTTTTATAAGAGTATAAAGGATTGTTAAAAAAAAAACCCATTCATAAATACGTTTACTACTACGTTTATACGTTCCATTTTGTTGCAGATATTTTATCTGAAGTGAATCAATCGAAGGAATCAACATCAACGGAGTGCTCACTTAATTGTTATAAGTGCATCATTAAAACCAAGTACTATAACACTTCTATCAACTTAATTCCATTTTCTGGAAAACTGGAAGCTGTGCCGGAGAAAATTCTTCAAGCAACCGAGGCGTTGATTATTTACTTTGATTCACAAGACGTAAGTGTTTATAAAAGCAATAATAGTATATCTATTATATTACAATTACTTGCAGACCTCCTTTATCGACACTATTCCCAAGACGTTAGCGAAGAATGAACAGATTCAGCTGGGCTTTCTGCTAACCTCTTCCACTTCAGAAGAAGGTGGATTGTCCTTTGGAGAAATTAAGGAGAAAACGAATTTCCTTTTTGACATCATAACATTGAGAAGCAATGTTGAGAGTGACCCAGATGAAGCTGAAAAGGACTACGAGGAAGTTGTGGAGGGTTTGAAAAACGTAGTTTGGTCCAATGTCAAGTTTGGTTCTGGTTAGTGCCCAGGATTTGAGTTGAGAAACCAATAATCCATTTCGTCCTATTGATTTGCAGATCACAAGGAGGAAATCAGTGCCGACGAGCTGGATAGTCAGCTGAAGGACTTTGAAAACTTACTCCTCACGGCGCAAAATCTACG

 

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