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CRIMIC CR01573

Gene Galphai

CG10060

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:6976149..6976167	65D5	FBti0210437	86451

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCAAGGAAAGAGCAGCAAACCGAAG	AGTTCGTTGAACCACCCACCCATTAG

Images

Construct Design Sequences

sgRNA sequence	ACACGCGCCCGGCCGACTGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCTCGTCTGGGCAGTTTAT	GGGCGCGTGTTATGGCAC	AGCGAGAGGCCAACAATAGG	TCGGCACTCAGACTGTCAAC

 

Amplicon 5'

Amplicon 3'

GGCTCGTCTGGGCAGTTTATTATATATGTAGTACTATGTATATTATCTGCTGCGTCGAATGATAAAAAAAAAAATATGAAACATAATCGCATTACGTGCTCGCCAGTTTCCCTTTTCGCTGAAAACTAAAGAAGTTGAAATCGCAGAGCAAATGGGCAAATACAAAAATTGGCAAATCTGCCGGTCCAAGTAATTTCGAATATCTATAGCTATATATAAACATATATACACGATGTTGGGGCATTGCAACAATGGCGCATTAAGCCGACTTTTTGCTGCAGTTGGTAGTTGGGTCCATTCCCAGCCACCGGATGATTAGGTAATTGCCAAAGCCAAAACTGCGACTTTACTTTCACTTTTCTCAACTTTCAGGGCTTCGATACTTGCACTGAAACTTTAATTGTTTTTACTATTCGTACGTATATCAATCAGCCATGGTTGAAGTTCCCTTGGGGCGGATGGTGCTCATTCAATGAAGATTGGTAAGTTTAATACATATGTTACGTACATTTATGAAGATTAAGAAATTCAGCCTATAGTTTTAGGTACCATAATGTGTTAGAATAGGGTCCACTCTAATTGTAATGTGCTGGCCACTGTTACACCTACTGTTAATATATATTACACTAAATGGCCACTGTTACATTCACTGTCAATATATATTACATTAAATTTGTTTTATAAATTTTTTTGAGTTATTGTTAAGACTTCAATTTTAACCAATTCCGAACAATGCTAACTAATTTTGCCACCAAAAAGTGTTGGAGCGAAAGAGACGCCTGCTGAATGCTGATAATGGGCAAACAACGGTCGCCGCATAATGATGAAATTGACAGATTTGAATACGAATATGGACATAAGGCGATGTTCAAACTTGCCACCGTGTGGAAAAAAGTAGCGAACAAAGCAATCCACAAATGAGCGGCTGGAAAAAGGTCAATTTAATAATAACACTACTAACAAAATGCGAAAGAGTGTGTGCGTGTGTGTTGGTGTGGCTGGGCCTTCATTTATCAATGCACCGTTAGCTGTGCCATAACACGCGCCC

AGCGAGAGGCCAACAATAGGGCTGGAGCGAGACGGGCTCAGAACAACGGCGATACGCACAAAGCCGCGTTTACAAGCTCATTATCATCGCGATGGCCACAACCAAACGGTATTATAATTAACATCATTAAACAGTGAATATCGGCGAAAAAAGAATCGGCGGGGAATGCAGGAATTCTAGCTTCTATTGATATATTTTATAGTTGTTAGAAGTTGCTAAAAAAACTTTTTATTATATTTATTAGAATTTGAAATTGTAAACAACATTCAAATATTTGTTCAATTTGTCATAAATGATTAAAAAATCACTATTTAGCTAGATAGGAAATTTAAGGAAGACATGCAGTTTTTACTGCACCAACCAATTCGTTAGATAATTACTTATCGAAATTAGAATATTCATTGGAAACAGGGTGACTAAGGCTACGCCATGCGATGATTTAAAAAAGAGGGGAGAAGAAAGAACAATAGCTGTAGACAGCAAGAGGAGGAGAATCGTAAGAAAGTGTTTGCGCCATGAGTAATCAAGTTAAATGGCGCCTGGCCTCAGTTATCGAAGTGGGAAATGTGTTAATCAGCGGGGAGTGTGAAATTGAGCGGACCCACCGAAAAAGTAAACAATTAAATCAGATGAAATGCGGCCCCAAAACGGAAGCCCCCCACCTAGTAGTGACTTTCACGCAGATCTCTCGATTATCATGAAATTTCCTATATGTGATGTACATACATATGTACATCAATTATTTAACCACATATAGTATATTGACGTACATATGTATAAGGTCGCTCGCTTGGCGATAATTTTGATAAGCCCAATGATACTTTCAGTTTAAATGTGTTGGTAAGCGAGTTCTTAAATAATTGTAGATTATTAAGTTGCTGTGTGTTGACAGTCTGAGTGCCGA

 

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