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CRIMIC CR01574

Gene PMP34

CG32250

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:4319309..4319315	64B1	FBti0210438	86452

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAATAGCTGGCAAGCAGCAGAAGAG	AGAAACTCAGACTGCCCATTTCACCG

Images

Construct Design Sequences

sgRNA sequence	CAAAGCGTGGCCCGCTTATCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGGAGGGCACTATTTCTGT	CGGGTGATGATTAGTTACAGTCC	CGGGCCACGCTTTGATAATT	GCCGGCTTCTTCCACTGTAA

 

Amplicon 5'

Amplicon 3'

GCGGAGGGCACTATTTCTGTGAAGTCAGCGAAGGCGAAAAACTTCGTTTTCGCCACGCTAACGGAAGAGGAACATGAGGTGGGAACTAACTATGTTCTTTTGATTACGATTCCTTCTAAACCAAATTAATAACATTCCAGAATCGAAAGAGAAAAGCTGCCGATATACTGAACAGCAGCACTGAGACGGGGGTAAACTTCATAAAGAAACCAAAGTTGGAGCCTCGGAGAGACAAGTGCCTAATAGATCAGCTCCTTTGATCCGTTCTTCTTTTAAGCTAAGACTTAAATGTACATTTCAAAAGTTAGACTGCTAAGATAGTTTATAGGAGTGAATAAAAATAAGTTAAATTTTATATTTCTTTAGATAGGAACAGTATAAATTGGTTATACTGCCTTATAATTGGAATATTCCTTACATTTTATGTGCGATTGTTGTGAAATTTGGTATGTAGGAAGTTTATGATTAGGAATTTGTATGTATTTCAGTAAAATGAGGAATAATTAATAAAAAATTAAGAAAGATTTAAGATTTTACAAACCCAGTTAACGGTCTATTTTGTAAAATATATACATATGTGCGTATATATATTTTGGTATATTTCTCCAGCTACATGGTATATTTCGAACCCACAAACATTGGTCATACTGTGTGAATGAATGTGAATTCTATACAGAAATTATGAATATGTAAGTGAATCATTTACAAAGTCGCCGTGATCCAGGAGGCACAACAAAGCCACCGCCTCCTCCGCCGCCGATAAATTTAATCTAATCTACACATCGCGGCGAACAGCGCTGCCGCAGCTAAATTTTCAACAACCGGCGGATCGATCGGGTCGTCGTCAATCAGTAAACACTCGCCCGAAGACGCCGCCGGACGTTCCAGTCCCCAGTTTAGAGATCTCACAGTCCGACAAACAAACAGCAGAATGGTGGCCCCCTCGAAACTCAGCCAGGTGCTTAGCTACCAGAACTTTGTGCATGCCGTATCCGGAGCTGCGGTGAGTGGGCAAATATTTGCCGGCTAAAGGTCAAGATGGCATCGGTGCTAATTTGCATTTGGTCACCTATTCACAGGGCGGCTGCATCGCCATGAGCACTTTCTATCCACTGGATACAGTGCGTTCCCGCCTGCAACGTAAGTTTACCCAGGGATTCAACAATCGGACTGTAACTAATCATCACCCG

CGGGCCACGCTTTGATAATTCATATAGGCGCACCTATTAATCTGTGCGCGAGTTCAAACTCCAGCGTTTGAAACTTTCTATTGACGATATGTTTGAGAGTCCATATCAGTCAATTGCCCGGGCTAATCCAATTTGAGGTCTGGTTATAGATGAAATTCGATAGCGTCTGGTTTCTGTGCCGCGAAAAAAATTGTGATAATTATTTTCTGTATAGTATGAGGAATAACTAAAGGTTTATTTTAAAAGATATACAAACACTATAGATGATATCATATGTGAACGTAGTTATAATATTACATTGTGTATATTTTGATACACAAAATTCGTTCAATATTAAGTGCCCATATGATACCTATGTGATATCGAGGGTTCACTTTACAAGGTGTGCGCTCTATTTCCCATTTTCCAAGTACACGAACAGCTTATATTATCTGAATGCATAAATTTTGCAATTTTATAGAGCCATTGGACTTTGACTCGTAAAATAAAACTTCTTACAATTATGATCAGTTAAGTTCACTAAAATTCGCGAAAACAAAAAGAAATACCTTTTAATACATTTTTTTAAAGGTGCAGCTTTATGACAATTTTAGCCCATACCATATTTACCCAACACGGTTGCATATTCGACTAAAGCGTTTTCAAATTTAATTCTAGAAATTGGATAATTAACATAATAGGGTTCAGGGTTGGCTTAATATCACTGACTCGTAGTATGCGTGACAAGTTCAATTAGCGCGAAAACCAAACACACCTGAAATAATCTTGGCTTCAGAATCCCCAGCTTAAAACTTATAGTGTTTTTTCCAATAAATATTTACAGTGGAAGAAGCCGGC

 

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