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CRIMIC CR01576

Gene Alp10

CG10592

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:5595046..5595079	64D5	FBti0210440	86454

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTATAGAAGCTTCGCTTGCCTCCAC	TGGAGCTGATGATTTCACCATCGTGC

Images

Construct Design Sequences

sgRNA sequence	GCTGTAGATAAGCTGGGTCGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTTGTTGCTATCGCCCATG	TTGAAAAGCTCTCTCGGCTT	GGATTCGTTCGGCAGTTCAC	CAACTCCTCGCCTCCAATGT

 

Amplicon 5'

Amplicon 3'

GCTTGTTGCTATCGCCCATGAAAGCACGTGTGGCCGCGATCGTGTGAACGGACATGCCATCGCCCAAAAATAGGATCACGTTCTTGGCGCGATTCTCGTTGAGCTTTTTATGGCCGGCCAGCTTATCGGCCAGAATCGATTGGGCCTTATCGTGCCAGAATCGTGTGTCCAGCTCCTCGTCGAGGGCCTCGAAGTCGCGTGATGATTGCAGACGGGGATGTTGGGCTGTAAAGTACGGACTTTGGGTTAGCAACTAATCGGCGGGAAAATCGGTCTAATAAAACTACTTCTTGAAGTGGTAAACAAGTTTTCAGCCTATCTCGAGCAGATCTATAAATCAGTTTTAATTATAAGCATTTATGGCCATTTGGCTGGATTTCCTATCGCTGGAAATTCACATTTGATTGAGGCTTAATAAAACGTGTTGAAAACGTTTTAGCTTATTATGGTGCTTTGTCATCATTACACTTTCGTTTGAAATACTTAGACATATACTTTGAGGAAATGTTTTCTTTCTGTACTGTACAAGCTTAGAAAAAACGCCTAAAATTAAATTGATTCTTGTTTAGTTCATAATTATTATAGCTTATTATTAATAAATAATAATTATACCCATTTGAAGCTTATAGTTAGAATCGTTAGTAACACTTGATTTAATCTAAGAATTTAGCCATTTTTGTTAAACATAAATCTATTTATAGGAATGTCACCCTATCTTTAATTTGCCTTCACGTGAACAATTGAGTCGTAAACTATTATATAGCTAGTCGATGACTGAGTGCTGTTTAAAATAGATCTAGATTAGCCAAGTCTAATTGGCCATCAAGATCTCTCTACGATAAGCCGAGAGAGCTTTTCAA

GGATTCGTTCGGCAGTTCACCACGCAATGCCGTTCGGTTCCACTTACCGTGCTCTTGGTCATCGGCTAGATCACTCCGGGCCTGGATGACCAGGAGAGCGAAGAAGCACAAGATCAGGCAACTCTTTACCATTTTGGGGTAGGGGCGGTAACTTTAAACTGAGGAATGCTCCGTTTGGGAGTAACCGCGAGACTGGCCAAAACTGACGAAATGGCCCGGTTATATAGCCTCCGATTTATCGAAGCGGTCTTCCTGTAATATACATACGCGTTATGGGTGATTGTTTTTGTGATTTGAGCAATCTAAAGTGGCGATCGATTAATTATCTCGAGTGCCTAAAAACGTGAGTATCACGGTCTGGCTAAGAAGGTGTCGTAAATTACCCGATCCCCACCAAACTGCCCTATCGACTCCTCACACGAGCTGCACCTGATGGTATATTAATGGGTCGCATGGAACTACGGAGTACAGTATTCACTTGGCTGGCACGGTGAAAGTATCTCGAAAATGCTGAGTCTTAAGTTCTGGATTCGCCTCGGTCTGCTGACCGTCGTTTGGGGTGCAGCGGTTCCTGGAAGAGCCCCCGTGGACGAGGAGCGAATGCATCCACATCTGCCCACTAGTCCCAGGCTGCGTACGATTTCCGGCGAGGAAACCCAGGAGTTCTGGCACTCGGCCAGCAAAAAACTCATCCGCGAAAAGTTGGAGTTCGTGAGGAACACCAAGAAGGCCAAGAACATTATACTGTTTTTGGGCGATGGCATGGGACTGGCTACTCTGGCTGCTGCCAGGAGTTACATTGGAGGCGAGGAGTTG

 

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