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CRIMIC CR01577

Gene Gbeta76C

CG8770

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:19689419..19689452	76C1	FBti0210441	86455

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAAGCAAGGCTGATTGCACGTTGGC	AACGACGTGTTCTTCGTGACCAACCT

Images

Construct Design Sequences

sgRNA sequence	GGATTCGGTGATAAAGAACGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GACAACCAGTGCACCGTCTA	ATCCCCCAAGTGCAATGGAT	GCTAATGCTCAGGCTGGCAC	TTCTCCGTTTAAGGCCGTCC

 

Amplicon 5'

Amplicon 3'

GACAACCAGTGCACCGTCTACGATGTGAACAATCGCGATGCCTCCGGAGTGGCCAAGATGGTCAAAGAGCTGATGGGCTACGAGGGATTCCTCAGCTCCTGTCGCTTCCTCGACGATGGCCATCTGATCACCGGCTCGGGAGATATGAAAATGTAGGGCTTTGCTTAACTTTTTGGAGTAAAATCTAAGTTTCCTATGACCAACAGATGCCATTGGGATCTGGAGAAGGGTGTCAAGACAATGGATTTCAATGGACACGCTGGTGATATCGCTGGCTTATCGCTTTCGCCAGATATGAAGACCTATATCACTGGCTCTGTGGACAAAACTGCCAAGTTGTGGGATGTGCGAGAGGAGGGCCACAAGCAGATGTTCTTCGGTCACGACATGGACGTGTCATCCGTCTGCGTGAGTACACATATATGTTTTAATAATAATAGCTTATATTAATTACCAAGAACAACTATAAGCAGCTAATCTATTTAGTACTTGTAATTTAATACAAATGTATTCATTACACCCACCCAATTATCATTATCGGTTGCAGTACCATCCCAGTGGCTTCGGGTTCGCCTCCTGCTCGGAGGACCAAACGGCGCGCATGTACGACCTGCGGGCGGACCAGCAAATCGCCCAGTATGAACCGCCCCAGAAGAACACTGGCTTCACGTCGTGCGGTGGGTTCTCGTTCTCCGTCTTGGTGGAACACGGTCGCCTTGACTTTTGACGGGTTTTTAATCTGATTTTGTGTCCCCACCAGCTCTATCGACCAGCGGGCGCTACCTCATGTGCGGCGGCATTGAGGGCAACGTGCACTCGTGGGACACGATGAAGCAGCGGCACACGGGTGGGTATTGGGAATTGGGTAGTTCGTATTGGGTATTGGATATCCATTGCACTTGGGGGAT

GCTAATGCTCAGGCTGGCACTTGACTTACATCGATTTGCTGACCTTCTTCTCCACTTTCTACTTTCTACTTACCATTTGTCACTTTCCATTCCTCCACAGGCACACTGTCTGGTCACGAGAACCGCATCACTTGCATCAGCCTGTGCCCCAATGGCATGTGCCTGGCGTCCACCAGTTGGGATCAGCAAGTGCGTCTGTGGCTCTAATCTGTGCCTCTGATCTGGGTTCAGTGTCGTCCAAAGGCGAAGACAACGTTTATGCGTGCAGTTTGCTTGCAAATTACGTATACGTAATATCCATAGCCTGCAAGTAATAAGGATAGTTCTCAGTGCAGACTAACAATATTAATACAATATTAAATCTATTGTTAAACTTGCGAACATAAGAATAGCTTGCCCCCTTTAAGTGCTAATTTGCAAGCAAAACGTGCGTATGAATATTATTATAATAATTGAATACTTGATTACCTAGAGAAAGTAGCCTCCAATAACAAGCAAGTGCCAATTGGATGTGCGAAATAAAATGTATGAATAGTACAACAAAGTAACACGTCTCCTCCATTAAAACTCCATTGCAACTTAATTTTTTTTATATATTTTTTATATTTTTCTTATAAAAAATACTTATACAATTACGAAGATATTTTTGCTGCTGCGATTTTTGGCGCTTGGTTTTGTTTGTTTGTGGAAATTGTCTTTGAGCTGGTCAAAGAGGCAGCTAGGTAAATTAAAACAAATCACTTTTGAAATCAACAGAGAGATAAAGATACAGGTTCAGATACAGAATAGTCGAAAGTATACAGATAGATAGAAGATAGATAGAAAGAGAGAGAGAGAGAGAGAGAGAGATAGAAATGAAAGTCGCATAAATAACAGATGGATAGAGATAGCTTAGGATCACACAGGAAGCAGGATGCAACGATTTGAGTATTCTGTAGATGGATCAGCATAGATCAGCTGGTACAGTTTGTTTAGTTTATCAGACTCTCGGTGGAACTACTGTGAGTTGTTCTCCTGTTTGGGTAGATGGAAGGATAGCGACGACGGGACGGCCTTAAACGGAGAA

 

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