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CRIMIC CR01579

Gene Ythdf

CG6422

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:25037000..25037025	96B17		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AGTGCAGAGCGAACTTGAGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGGAATAGTTGCCCGAGC	AAATCCGCCTCCATTGCAAA	GGACACCATCCCCATCAAAA	CAGCACTTGACTTGCACTCG

 

Amplicon 5'

Amplicon 3'

ACTGGAATAGTTGCCCGAGCGCTCCGAGTTCCGGCTTGGATGCGGCCCGCCGTCGTGTCGTCTGGCGTTCGGTGGCCCAAATGGACCGGCATTGGTCGCTGGACGTGGGGCGGCATGGTGGACAGGCTGCTGGGGCCCTAGATTCCTGCGAACAGGTCCCGTGGGCGAAACCTTGGCTCTGCTGAAGTTATTGTTATTTCCCAAGCCCTTGTGCTCGTATTTCAGGAAGTTCTCCGTATCCTTGTGCACTTTCTCGGCTCGTGCGCCCAATGGCGGTGCCGTGTTGCTCGATATGGAGAAGTCAGAACTCAAGTCGGAATAGCCCAAATCGATCGGCGAGGGTGGAGATGGTACAGGAGGTGGTTTGTTATTCGGCAAATCCCATACGTTAACATCTAAATTGTGCTTTCCCGGAACCATGGGTGGTGGTGGCATGCCAGGACCCTTCTTCTTGCTGTTGGAAGTGGTGGACGCTGCCTGTGAACGTAGACAGGATATAAGTGGGTTTGCCCGCTGGCCCAATGAGTCAGATACTCACTCTCGATGTTAGTTTGGCTGGCTGGGAGGCAATCGAGGCCCAGGTTGTCTTCTTGGGAGCGGCGACAACTTCTTGATTTTGTGCTTCATCGCTTGTTCTGTTGTCCAGTTTTGTAGACGGCTGATCGTTTTCATTTCGAAACGACTTAGGGGTTTTACGGAAATAAACATTAGTTTAGGGATAAATTCTTTCCGAATATGGTTTCAATATAGATAAATAATAATATGTACCTGTGCTGCTGGCTTCCTGGAATTCCAAGGATTTACTTGGTGCTCGTCGCGTCGTGTGTTATTGGGAATACTATGACCATGTCTTCTATATGAATCTACAAATATCGTGAAAGACTACATGTTAGAACAATTTTCAAGAAAGTTTGATTGCTTAGACAAATATTACATTAATTATTTACATACGCCACACCTTCACGCTTTAAAGTTCATGATTTCTTAAAAGATCCAAAGAAACTTGCATTATCTGTGGGTGAAAATGCATTTAAACAAATTTAAGGAGGCACAGCAACTTGAATAACATTTCGTTTTTGTTTTACTTTCTGGCAAACAGATTTTACGAAAATTATCCAAATTTACTTTTGCAATGGAGGCGGATTT

GGACACCATCCCCATCAAAATTGGCATAATTGGAGAGGCCAATTTGGACAAGCCCATCTACTTGGCAGAGCGCATGGAGTATGCAGTATGCACGCCGTTTGGAAAACCATCCGATGTGATCATCGATGGCCAGATAGAAGGCGTCAATGTGTGCCTGCTATCACGAAATGGACGCAACCACGACATCATGCCCAGCAACATTAACTATAGGGCGAATGTGTGGGCCATGCGCAAGATGGGCTGCACCCACATCCTGGTGACCAACACGTTCAGTTCGCTGAGGGACACCTTCCAGCCGGGACACTTGGTGGTGCCAAATGATGTGATAGATTATACATCCAGACGTGCTCAGACCTTTTACGACGGTGCCGTGGGTAGTCCATTGGGCGTCTGTCACGTGCCCATGAATCCGACCTTTTGCGAACGCACCAGGCAGCACCTTCTGAGTGCCGCCGAAGAGCTGGGATTCCCCACGGGATCCAGCGGCACGGTGCTCACACTGGAAGGACCGCGTTACTCCACCGTTGCCGAAAACAATATGTTCCGCAAGTGGGGCGCCGATCTGCTGAGCATGACCCTGTGTCCCGAGGCCATTTTGGCCAAGGAGGCGGGCATTCCGTACGCCTCGCTGGGTCTGGTCACCAACATGGAGTGCTGGTGCGCCAAGCAGCCGAACGCAACCACTCACGAAATCATCTACATCTTTAAGAAGCAATCCGAAAACCTCCAGAAGGTCCTGATAACGGCTATTCGGAATATGGCTGCCGAGGATTGGGCCGAGGATATTCTAAAGGCAAAGGTAATGTTGATTGCAATGTAACTTACATAAAATTGATTCTAAAATCAATTTTTCTTAGATTCTGGTGTGCAGCAACTTTGCTAATAGTAAATGATGGTCGGTCTTGTGAAAGGTATTGTTTTAAAATTATGCAAATAAATAATATTAGCGTCCGTTTTGAATTGTTCTATGCGTTGGCGCAAAGCTACTTATCTTGATCTCTAAGCGATCATAAGCTGCAATGAGCCAAGTGAATCCAATCGGAAGCTAATCGGACTAATCCCGACTTCCCGGTGCACCGACTTATGCATATTCGCAGAGTCGAAAAACCAGTTAGTGAGAACGATTACGCGCCGAGTGCAAGTCAAGTGCTG

 

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