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CRIMIC CR01580

Gene boss

CG8285

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:25963670..25963676	96F8	FBti0210442	86456

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCAAGCTATCCACAAGTGCCGAACT	AATTCATCGTCCGTTCTATCGGTGGG

Images

Construct Design Sequences

sgRNA sequence	ATGGAATGAATGGCAACTTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTTGAACTGGCTTCGGGGT	GCCATTCATTCCATTGCACT	GAGGGGATTTCTTTTTCCGGG	TCCAGCGTTTCGGTTGCTAT

 

Amplicon 5'

Amplicon 3'

ATTTGAACTGGCTTCGGGGTGTACATATTTATTTGTATAATTATGGGAAATTGTACACTCGACGTGGAAAGCACTCAATTTCGTGAGTGACTGTTTGAGCCAATAACCATAACAACCAGCGAGTGCCGGGCCTCAGCCAAGTTAGTCAAAACAAAACGGCCAAAACAGGTGCAAAACATTGCCACATCAGTGTTCCGCCTTCGGTTTTCCAGGGGGTGGGTTCGAAAACGGAAAAACTAAAATAAAATAAACTGAAATAGAGACAAAAAAAAAATCGAATAGAACATAATTGCATTGTGCGGGCAACTGCAATGCTGCACCCGTTAACACAGGGGCTTACCAAATCAAATTGGTTGTTCGTGGGCCAACAATAAATATATATGTACGGTTTGAGTGCTGCGTACATCACTGGACAAATATAAAACGAGAAACGTTTAATTGACCCTAAAATCACTGAGGCGTTCAATTTGTGTTCCCTTGTTGTTTTTTCTTTGTTTTTTTTTTTTGCCAATTGCTGTTGCCCAAAACTATTGTGGCTCTGACTGTCAGTGGGTCTAGTGCGTGAATCTGTGCAATAAGTGTGTGCAGTGCCATAAATTCCAGAATTCGGCTTCCACAAAAAACCTCTATTGATTTTCCCGAAGTGAGAGAATATGCGAAACAGAAATTTTTTCCACCGTATTACTTTGCACTGATAGTGGGCAAAATCACGAAGAAACAGACATGGAAAAGTGGAAACTATGCCAAGCCAACAAAGTCTGTGTTAGTGTGACAATTTTTAATGCGATTAAAGTGCAGCACTCTCCACGCAGTCGGCAAAATGGTTGAAGTGGCCGGCAAAAAATAAAAAATACAATTCTGCACACTGGCTAATATCCAACTTACCATTTTCTTTCTTCCTTCTAGCTATCAAAATAAAAATAAAATGATATCAATTATCAGTACATGTTGAAGGAAAGACTGCTGCATTGCTTAATTATCAACGGACATTTTTTGTATTTAATTGTAAAACCTTTAAAACTTTAGAAATGAAGGTCATGGACGCTTTGCAATCTGGACGGAGAAAGCCATTACCAGTGGGTGAGTGCTGAGTAAATGTCGTAAGGCAGAAGTGAAGTGCAATGGAATGAATGGC

GAGGGGATTTCTTTTTCCGGGAATATTTCAAGACCTGTATTTTTCTCAAAATGCCATTCAAGCTTGAGTTTGTGTAGAAAATTCCATTGCAAATATGGTTTAAGTGAATTCCTTTATTGAGTAATTCAATCAATTAACTAGTCAATCAACCATGCTAACCAGAACAAAGTTACTTTAAATTTAAGGACGACGATATTTTCCATCTTATACGAATAAGATTTAATTCATTGTTGTCCTCTGCAGCTCTTCTATGCATCCTAGTCACTGTGTTCTGCGTCTTGGAGTGCCATGGAGCTGACCTGACATCACCCACCAAGAAGTCCGCTCCCCTTCGAATAACCAAACCACAGCCCACAAGTCAGCAGGCCAAGCCCATCAGTATTACCACAAGGGCCCCAACAACGGTGGCATCAACCACCGATGATGAAGTCTCCAGCTCCGTCGATGGTCAGCTGGCCCCGCTCATCTCATCCACAACCGAGGGACCCAGCAGTGGAACAACTGCCAGCTTAGTGCCGGAAATATGTCTCAATGGTCTGCAGTTGACGGTCAATAGTGCGGATGAGGGAACGGTTATACGGAAGCAGGAGGAGTTTGTCAAGATACTCGAAGGAGATGTGGTGCTCAGTGTCTTGACCAAGGACCCCGACTCCGCGTTGTTCGTAATCAATCGTGTGAACCAGGCGAATCTTATAATGGCAGACTTTGAAATTGGTTTGTAAAATTCCGCTATTTATATGCACCTAAATCAAATCCTTCTTATTGAATTCCAGGAATTCGCGCCATCAGCATCGACAACGCCAGCTTGGCAGAGAACTTGCTTATTCAAGAAGTTCAGTTTCTGCAGCAGTGCACGACGTACTCGATGGGCATATTCGTTGACTGGGAGCTGTACAAACAACTGGAGTCTGTTATCAAGGACTTGGAATACAATATCTGGCCAATACCGGGAACGCGGGCGCACCTCTTTCCCAAAGTTGCGCACTTGCTGCATCAAATGCCTTGGGGCGAAAAAATCGCATCCGTGGAAATAGCAACCGAAACGCTGGA

 

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