• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01582

Gene Pi4KIIalpha

CG2929

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:5560523..5560553	83B2	FBti0210443	86457

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAACCCAAGCATTTCCGTCCTCCAA	ATCCTTGTAACCCTCCACGAAGAGCT

Images

Construct Design Sequences

sgRNA sequence	ATTTCATAGGTAGCGTGGAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCAGCACAGCTTATGCATC	AGCGTAGTCAACAGTCCTATGC	AGTTGTCGTTGAAATCGCCC	AGTCATCGCCCCACATTTCA

 

Amplicon 5'

Amplicon 3'

GGCAGCACAGCTTATGCATCCATTTCGTCCACTTCGGATTCAGTCTGCCATAGGGTTCCTCGTCCTTTGGCTTAAATACGGCCAGGCATTGCTAAGGGGGAGCATCATAAGAAATGGCATAATAAATACATAGAGCATAAGCGGGTAAACAATATACAATTCACTATTTTGAAACTGTTTCAGGAAAAAACCAAAAGTTTTGCAAAAGCCTTATATTTGCAGTACTTCCCCGATTACCCGATCTTATTTATCTATGCGCGGAAAAGTAAAAGATCGACGAAGTTAGGCCCAGAACTGGACGACCCAACTCCCACCTTATGCGCAAGAACGGCTAGAAGATCGTCACCCCGATTCCCTACTCACATGGGAGGCATCCTTGACAAAGTAGGAACCACTGCTACCCTGGTAAATGCGCTCGGGCAGCACACCATTCTCGATGGCCCCCTCCGCCTTCGCCACGATCTCCGAGAAAAGCGGGTCATCTATAAGAATCAAAGATTAAAATTAAAAATAGGCATTAGCACTAGGGCTTTAAATTACTAAAAAAGTTTGTGCGTAGAACGAAGATTCGCATGCCTCAAGCGACGGGTAAAAAACGGACTGTTCGTCATTTATTAAAATTGAACAAATTTGCTATATAATTATGGTTTTAATAATTTATAAACACACCAACGAAATAACGTGCCATCTGTTGGATAAAAATAATTACAGCAAACATGCACGAAAACGCTTATAAGACTGCGATGAAATTCAAGACCCCATCCTACACTTTACTTTATATCAAGGAATGGCATAGGACTGTTGACTACGCT

AGTTGTCGTTGAAATCGCCCTGCAAGTCCTGGCAAATGGTAAACACTTGCGGTATGAAGTTTTCGCGACTGCTGCTCGACAAGCGCGACATGGAGGCACTGGTATATTAAGTGCGCCGGCAGTCGAGAGGTTTCGAGTCGGCTGGCAGTTGATAAAGGAGAACCAGGTCACAATTAGCATAGCCAAAGCGTTTCGATAAGGGCCAACCCCTTGCCACCACTTACAGAGGTTCCTCAGCGGGCAGTTGGCACAATTGCAGCGCGGAGCCGCGATTTGAACGGTTACTGTGTCTTTGGAGTCACGCGCCATCGACGGTGAGTTCACCAGAAAGGGCGGATCGCCCTCCTTGTCCGACTGCGACTCCAAGAAATCCTTCTCCAGCGGGCACTTGTGCTCGCAACAGATGCAGGCGTAGGCGGCGGAGGCGGAGGGCACCGAGGAGCTGCTCTCCAGCGCATCCTTGCTTGTCGTGGGCTGCTCATCGTCTCTTGGTTCGCTTGGCTCTTGGTACTTGGCTGCTATAGCGTTCGGCAACGGCACCTGGCTGAGAAACGACTTAATGTTGTTTTTCGGCATGGGGCTATGTCCAATTGGCGACAGTTGCCAAAGGAACAAGTTCCATTTTTGAAGTGCCTTCCTCGAAATTCTTTATATGCTTCCCGACTGACTTCGATTCCGAGCTACAGACGTCCACGGTGACATCCAAGTACACCAAGATTTATAACACACAATTTTGTGCTCAATTTCGAAAAAAAACTAATTCTTGTAAAAACCATAAAATTGTAAAAAATGCTTTGAAATGTGGGGCGATGACT

 

---

Search Again