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CRIMIC CR01586

Gene Pkc98E

CG1954

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:29044279..29044303	98F6	FBti0210444	86458

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTCGTTGTTTGCTGTTGTCGCTGT	GGGATAGAGGACATCGTCGTGCATAA

Images

Construct Design Sequences

sgRNA sequence	GTGCTTCTTCGATTCTTCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCTTGAGTCGGAACAGGTC	AGTGCAGGCCAATGAATCCA	CACCAAATGCCCAGATTGGA	TCTGTCATGGTGCAGTCGAC

 

Amplicon 5'

Amplicon 3'

TGCTTGAGTCGGAACAGGTCCTGTCCACTGTCCCACTGCACCATAAGTGAAATCGATCTGTGTGCTCCCGTCCTCGTTCCCATCACATTGCATTTTAAACTCACGTTTAAAGGGAATGCAGTACTAATTAGATTCTATTCCGATCGGAGTTACAGAATATTCTTTATAGGTCTAGAATTACAGATAGTCTTCATCGCATACACACTTTTAATTTGTCCGTCCGTCAGATTTACTCAATTCCATTTGATAATCATGCATGCTATATATAGATAAGCCCAGTCTTTCGGTTCTATAATCCTAAGTCAGCATTTAATTTTAAGAGATGAAGACTAGACAGTTATTAAAAAACCAATGTCAATAAACATAAAAACGCGAATTCTTTAGGATTGGACTCATTGGTCTAATAAATCATCTTTTTAAATCTATTTCATGATGCACGATAAATTTCTCTTTGGTTTTTAATGAATGGGTTCAAAATGAGTATACTCCATGGAGTCGAATAAGAATAGAAATTTTATATAGGATTTCGTATTATTTACTTTAAATCATTGTCATGAATAAACTGAACTGCTTTTATGGCTGCCTTCGTCATTTCTCACTCTTTCAAGTAGTTTTGGTTCCAATATCAATATCTCTGGCACGAAGCATCTAAATGTTGTGATTAAAACGAAACACTAAAATTAGAGCACAGACACAGACCAAGCACAACCTAACTCCACTGACCAACATGTGAATAATCACACTGCACAGCAAGAAACAGTCGACGGTTTATCAACTAATTACTGTCCACCAGATCGTGGCATTCGAGGAGGATCACGAGCCCATTTGCTCTCAATCCGAGTCGAATGTCACAATGGCGGACTTTGTGTCTAACCCTGAATGTTTCAACGAGCTTAGTTTAAACGAAATCCTGTAAATATACACATTCACGTTTGTCTAGAGTTTAGGCTAAGAAGGAAATCGCCCTGAAACAACATTTACTAGTTTGAAATTATGAAATCATTGCATTTATTGAGCTTTGTATCTTTTGATAGAAAATGTAGATGGTTCATTTTGTGTATCATGGCGATCTTCTTTCTGAGCTCATCGATTTTCATAGAGTCTTGGATTCATTGGCCTGCACT

CACCAAATGCCCAGATTGGATACTAATGATTTCAACTTTGGCTTCTCTTTCCCAAAAACTCTAGTCTGCACGTTAGTTGTACATAAGAAATGTCACCTGTCGGTGGTGTCCAAGTGTCCGGGCATGCGAGATGAGGTAAGTGCCAATGGGATACCCCCACCAGATCGTAATGATCTAATCGCAAGCCTTTCCAGCAGCCAGCCAAAGTGGAGATGGTGCCGGCGGGCCAGAGGTTCAATGTGAATCTTCCGCACCGCTTCGTGGTGCACAGCTACAAGCGGTTCACGTTCTGCGACCACTGCGGATCCCTGCTATACGGCCTGATCAAGCAGGGATTGCAGTGCGAGACATGCGGGATGAATGTGCACAAGCGATGCCAGAAGAATGTGGCCAACACGTGCGGCATCAACACGAAGCAGATGGCCGAGATACTCAGTTCGCTGGGCATCTCGCCGGATAAGCAGCAGCCGCGCCGCTCCAAGTACTTGAACCAGCAGGGCGGCGAGGACAACTATGGGGCATCCCTGGGCGCCGATGGCGACGGTGCTCCGGGGCAGTCGTTTCGCAGTTGCGCCTTGTCGGTGGACAGCCTGGCCACATCGACGACGACGATGACCAGCGGGTATAATAGCAGCAGCTGCATGAGCCTGGCGGTGACCGGATCTGGAGGCGTTGGGGCCACCGGCGAGACGCGCCCGGGCAAGTGCTCCTTGCTGGACTTCAACTTCATCAAGGTGCTGGGCAAGGGCTCGTTCGGCAAGGTGATGCTCGCCGAGAAAAAGGGCACCGACGAGATCTACGCCATCAAGGTGCTCAAGAAGGACGCGATCATCCAGGACGACGACGTCGACTGCACCATGACAGA

 

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