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CRIMIC CR01587

Gene kar

CG12286

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:12684985..12685004	87C5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CTAAGTGCTATCCCACGATGCAATCC	AAGGTAACCAGGCCAATGGACACCAA

Images

Construct Design Sequences

sgRNA sequence	TAGAGTTGGGCCGCCATCCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAGGTTGACATCCTCTCCC	CGGCCCAACTCTAGACATGG	TGCAACTTCCAGGTGGATGC	TCTCGGCGAACGGTCAATAG

 

Amplicon 5'

Amplicon 3'

GCAGGTTGACATCCTCTCCCGGAAAAGTGGTTTTCACGAACTGCATCATGTGCACATAGGGCACAAAGTAGCCGAACAAGGCGAGCGGCACACAAAGTGCCCAGATGACGAAGCGTTTACGCTTCCAGATCTCCACGTTGATGATCGAGCGTAGGAAGAGATTGATGCGCGATCTGCCGGGCTTCTTCTTCGGCGGCTCCGGTGGCGGATGCAGTGGCTTGTACACGAAGGAGCAAAGGATAATAAAGGCGGAAACGAGGCTCATTATCTGGAAGACGAGAAGCCATAAGTATACTAGCAAGTAGATGCCATCTTTATTCCACTTACCCGCAGGGTCCCCTCCAGACCATAGCTACCGAGCAGCTTGTCCAGACAGGGCGGCAGTATCACAGTGAAAACGCTGGAGCCGGCGGTCACAAAGCCACTCACCTTTCCCAGGTACCGCTTGAAATAGTGGCCCAGGATGGCCAATGTGGGCGTGTAGGCAAGGGCGGCACCCAGGCCGAACATGATCCCGTAGGTGAAGTACAGGGCGCCGATGTTCTCCGTGCAAAACGAGGACAGCAGCAGTCCTCCCGCGGAGAGGACTCCGCCGGCGAACGTGGTCAGCCGCAGGCCAATCTTGTCCGTCAGACAGCCAGCCATTGTGGAGAAGAGGAATGTGGTGCCGATGGCCAGGGCGCCAATCAGAGCTGAAAGTAATTTCTACAATGATTAAGTATTCTTGATATCCAAATTAAGTAATCTGTGTGAAAACAAGAAACTTTGTGAAATCCAGAAGGTGTGCTTCCTTAAATACAGCTACATTTTGACCCATTTTTAAAGCTATGTTGAAATATTAAATACCTTTCATCTTAAGTAAAGATCATTTCAATATGTGGATGTGAAGGAATGTTGATTTGCAATCTCACGTCTTGCACAATATTTGGTTTTCTTGCTTATCAAAATTTATATTGTTAATATTGTTTATATATATATTGTTTCTAGATGGATTCCTGTGCATTTATTTGCCGTGAGGCAACTACCTAATCCCGATCTTGTTGACCAACTGTCTGCTCGACTCACTTGGACCAATTTCAATTGACAAATCGAATCTCACGCACTTGCGGTCGATTGTGAAGTGAGGCCATGCAAACCATGTCTAGAGTTGGGCCG

TGCAACTTCCAGGTGGATGCCCGACTGCGTCGAACAGAGTGTGCGAGCTGGAAACCCGATCCGCTGGCGGTTGATGGGGTTGGAAGACCAAATGCATCTTGTCGCACGCCGCTCAAAGATTCGATTTTCGTCGTCAATAATTTTCCGCTGAACTCTGGCTGAAGAAGTGGGGGAAATCCAATGGTTTTCGTGGCGTAAACACTTTCGCTTGTTCCACTTTGGCACTTCCAATTGATGTCCGACTGACAGAATTGGGTTAATTCTTTGTTGCCCGCCTGCGAGGATGATCAAATGCCGTGATAACGCAGTTGTGCAGCTGCAGCCGCGCCAGCGTCCCAATGGTCCATTGCCTGGGACATGGACAGTGGACCGGAGCAGGTAGCGCTTCCAAAGCAATTATCGCTTTATTTACGACTAATTGAAACCGTATAGATTATATTTATAGCACGCAATTAGAAGTCTATGACCCCTCAAAACTAAGCGGACTTGTAGTTCCAATCGCGGAGAATTTCAAATACTTCTATACGCCGGAACTGGAGATAAGCCCTCGATTCAGTTCGCGTGTCCGTCCGAATTTGTTGTCACAATCTACGTCGGAATGGCTTTTTTAATGGCGATATGCACACCATTCATAAGCTAACCTCCCTTGAACTTTAATCCAAAGATTTGTTTATTTCTTTCCATTTTCATTTTCATTTCAGTGTATAAGAAGTACGCTCCTCAGCTATCTTAAATTCCACATAGTATATAATATGGTAGAAATACAATAGAACAGAAAGTGCAACGCACATGTCCCAAAATAACACTTGACCCACTCCAGATGCCTCATGGATACCTATTGACCGTTCGCCGAGA

 

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