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CRIMIC CR01589

Gene Coq7

CG14437

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:6771608..6771624	6C13	FBti0212935	91263

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	CAGCAACCAGCAACTTGGATCAACAC	TCAGCCTAGATCTTCTTCGAGATGGC

Images

Construct Design Sequences

sgRNA sequence	AAACGATGACTCCAAGTTCTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCTCATGGTGTGGCGCTAG	GGCGCACACGATCCAAAAT	TGGAGTCATCGTTTCGGTGG	TTGGCGTCATGATCGTTGGA

 

Amplicon 5'

Amplicon 3'

ATCTCATGGTGTGGCGCTAGCATCACGCATTTGAATCCACCGCGCCACAATCTCCTAGCGGGTAGTTACCCGGGACGATTGTGGTAATTTTCATTGAGATACACATAGAATCCGCATTTAATGCTTTATGTTGCATCTCAGATCAATCACATATGTATATACATATATGTATGTAATTTGTGAGTTGCGGCACAGCTGATTGCGTTACTATCGGCTATCGTCGGATGATCGGTCAGGGCTGCAAATGTGTAAATAAATTCGTAGTGTTTTTGATCTAATCAAAACCATAAAGTTGAGATGTTCTATGGTGTTGCTGCTATCATTAAAATAATATGAAAATATAAAATAAATATATGAACAGTTTGAGTCAATGGTCAATGGCGTGACTCACGAACGTGGTGCGATACCGGTGGCAGGCACGTGAGGATGGCCCATCCCTGGATTGGATTGCGCAAAAATAAACAGAACGCAAATTGTTTTTGTGCTCGGCAATTCGGCGAATTGCACTTTACCTAATAATTTATTTATTCAACACAATACATCTCGTGGAAAGCTGCAAATTAGCAATGAGTAAGTAGACTGCACTCAGTGGCTCACAACTGACCCTCGAAATCTTCGCTAGATCACGACGCATTAACTCTTAGATTTTGTTTTTATTTAAAAATGTTATGTTAAATTACAAAAAAAATGTATCACAATTAGAATTATTTTCGTTTCTTCCTTTTGTCGCTGTTGCTGTTGTTCTTGTCGTGCTGGTTTAGTAGAAAAGTGAAAAGTTGATTGAATATTTTGGATCGTGTGCGCC

TGGAGTCATCGTTTCGGTGGACATGTCGTCGCTTGCCTGAAAACGATGAGATTAGCAAATTTTTATAAGCCCATCCTGATCACCGGACATTCCAGGATCAACGCTGCACTTACGACCGTTGCAGTGGCGTCCATAGAATCCCGGATGGCACTTGCAGTGCTTGTGGGAGACGCAGGTGCCATTGCGACACTTGCAGATGGAGCGCTGCTTGCGCCCGATCTCGCAGTGATCGCCTCGTAGTCCGTTGGGGCAGCGGCACAGGTTGTTCCCGATGCACCGTCCCTCGTTCTTGCAGGGGATCACACACTTGGCTGTGAGGGAAATGGGTGGGCAAAGGTTCAGTGTTTCGCCTCGATAAGAGTTGAGCCGTTTGCTACTCACAGTACTCGCAACGCAGGCCGTAGTAACCCTTGGAGCACTGGCACTTGTCCTTCTGTATACACTTGCCGCCATTCAAGCACTTGTCCTTGCAAATACCTGTTGATTACAATGGGATACTATTAGTTGCGCGCCAGGCACATTTCATTCTTATATAATGCCCAGTTATCTTGCCCCAAGTGTACTTAATCATCGCGTATCGTATCACCCTCCTTTTCTACGCAGATGGAATGCTGAGAAGAGGTCTTAGCCGGCCCGAGCCACGCCTGCTCTCCAAGCGCTATCTAAGCTCAGCAACTGGAGGAGGATCAGCAGGCACGACCGAAGGAGGCAGCTCTGAAACCACCCCGCTGCGTCCACGTCCAAATGCGCTCACAGATGAGATTATACGCGTCGATCATGCCGGCGAATTGGGAGCAGATCGCATTTACGCCGGGCAAATGGCCATTCTGGGCAACGGACCGCTGGGCAAGACCATCGGACACATGTGGGAGCAGGAGAAAGAGCATCGTAAACAGTTCGAGCAGCTCATTCAACAGCACCGCGTGCGTCCAACGATCATGACGCCAA

 

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