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CRIMIC CR01591

Gene CG9650

CG9650

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:7234417..7234418	7A4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGGTCAAGCAGGAACCCTACGATGA	GGTTTGGCACGTTCTGATTCTGTCAG

Images

Construct Design Sequences

sgRNA sequence	CATCTGTGTGACTTGTCAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATGGCGCATATTTGGGTGG	AGTTGGCATTGTCAGAGTCA	GACAAGTCACACAGATGCAC	GCAAATTGACCGCCAGGAAG

 

Amplicon 5'

Amplicon 3'

CATGGCGCATATTTGGGTGGCAAAATTAAAATTCCCTCCAAAGCAGAAGCAGGGTCGAATAAAAAATGTGTTTGATGTGATGATCGGGCCGCGGGCCCTCGGCGATAAGCTGCAGCTGCATTAAATAAAAAAAAACACCGAACGAAACCAAACCGAAAACCGAACCATGGCAAACCCAAACCCCTGGTTCTTCTTCAGGGGCAATGGGCTAAGAAAAAAGTACACATAAATAATAATACGGGGAAAAAAGCGCGGACACTGCGGGCTGTGGACTATGGACAACGGAATATGGAGTCCGGACAGCGGACTGCGGACTTCAAAGCGCAGCTCTCAATGCTGATAAACTGCAGCGGCTGTGGCAGTGGCAGCAGGACGAAAAAGGACACTCGGACAGCGGCGACAATCGAAGCCCCGACTCTCTACTTCTCTCTCTCTGTGTGTCGTTCTCTTTCCCTGGCCTTTGACTTTGACATTTCAATGCAAATGATGATGAAAAGCCAAAGACAGCAATACAAAAAAACAAGCAAGGACTATGGCAACGGTTAAGGGTAACCAGGCAGGACAATAAAGCCGCAGCTCTGCAGCGCTGTAGCTCGGAAGCTGCCCTCGAGGGTAATTTGAATGCCTCAAAAACAGCAGCTAAGGAATGCCAAAGGGGGTGAAAGGCCACTTGACTGGACAAAGGACATCGCACTGGACTTGACACACGCACAAAAGCGGGCCAAAACAACAAGGGATATTTAAGGGAATAAAAAACAAAATAAAATAAATATAAGTACAAGAATTCCCTCTACTTGAACTCACTGAAAGAGGTGCCCTGTCCGTTGCTTTTTGTTGTTGTCGCTGTTGTTTGTTAACTGCCCAAGAGAGGTCAGCATATGAAATCATCGCGAAGGTCGTGTGTCAGCCGAAAAGCGATTGACTCACAACATCAAATTGGCCTTAAAAATTGGGATGAATATATATCGCGATATATAGATAGACAGAAACAATAGATAGCTAGGGGGGGAAAGGCACAAGCTACACATACGCCATTTAACCCTTGAACTTTTGCGCTTTTGACACGAACAGCGAACAACAAAAGTCACTGACAATACGGCAAAAAGTTGTTTGACTCTGACAATGCCAACT

GACAAGTCACACAGATGCACAGATATATAGATACAGATCCAGATACATCTGGAAATACAGATACACAGATACAACTCGCACAGAGCTGCGTTAATTACGCATTGTTCTGCCTTTTTTCTCTTTTTTTTTCCTTTCGTTTTTTGTTTTTTTTTTTTTCTGTTCTGTTCTGTACTTTACTTTACTGGGCTGTCCAACCTAACTCCTTCCATTTGGGCCATTGTTGGCCATTTGGTAATTATAAATACGCCATGGCAACCAACCAGGCAGCCACAGTGCTCGTTGAATAAAAGAAAGCCAACTGTCAACAAAATGGATATTTGTTGCAAAAATGTAAATGCAACATGTCCTTGCCACAGGACTTTTCACCTGTCAGCGCGCCGTGTTAATTATCAATTTCGAGGCTCAGCATGTCGCGACCGCAGCGACAATGAAACCCCATCAAGGGTTAAGGGTTAAGGGGTTAAGGGTCACAGCTCAGATGGGGCGGGTGGGTTATGGCTCAGACCGCAATTATTGACTCATGCTGGCACTGAGCCAAAAAGAAAAAAAAAAAACAGTAGATAAGAGAAGAGGAGAGGAGAGAAAGCTGAGTGTATAAAACACCAGCGAATGGTCGAGTGGTCGAAATCAATGGCTAATGCCTTCGGTTCATGCAACTCCACGTCCTGCGGAGGATTAGAAGTCGCCCGGTGGTGTCCCACGTCCTTTTTTGTCCTTTGTGCGGAAAAAGCCGGAAAAGGATTCACACAGACCGACTGGCTGGCTTAGGATGCTGACAGTTGGCGAAGCTGCAACAATTATCCCATTACGCGCGCAACCCTTTCATTTTCTGATCGCCACCCGCTGGTAAAATCGCTCATACGCACTGTGAGACGAGTGCACTTCCTGGCGGTCAATTTGC

 

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