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CRIMIC CR01593

Gene CG8945

CG8945

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:17082507..17082520	15E1	FBti0210445	86459

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGGATTACCTACAAACCACTCGTCC	TTCCAGAAACGATCGTACTCGTGGCT

Images

Construct Design Sequences

sgRNA sequence	CGTAGGGCGCTCTGTACAGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAGCGTTACTCCTCCAGCC	AGGTGGGCCTCCTGATCC	CTACGAGGAGCTCAAGGTGG	TCAGGGATTGGCCTGGATTG

 

Amplicon 5'

Amplicon 3'

AAAGCGTTACTCCTCCAGCCGGACGGTAATAAAGCACCAACCATGACATCCCTTCTGGGCGAACGACTCAGAAGAGTTTCTCGCATCGCAAGAGCTCCGCACAGCCTACAAGTTACCGAAAATCCAGAAGAAGGTGGAGCACCAATCGTACCACTAAGAGAATCCGGCGCATCGGAAGTGTCAGAAGTAAATCCGGGGGTTATGGTCATTCCGGAGTCGGCCAGTCCCCGGGGATCGGCGGTGCATGGACAACACGCTAGCTTGGTCTTCAGTGCTGAAGCCAGGGATTCGGACAGTACGCGGATATCGGAGGAAATGGTGGAAGTGTTCCAAACCTCTTCGGCGAGAACCACATCCAATGAGCCCGCAGGGGGCAATCTGCGCTTTGAAACCACTCCCGTGGCTGTAAGCGAAAGTATGGTTGGCAATATAAACTTCTCCCCGAACGCCATCTACGATCAGAACAGGTATAGCAGCATGATGTCCAACGGGGATTACAATAATGGCAACAGGGGTGAGAGTGAGAGGAGCGTGGGAGCCTGCCACCATGGTTGGGCGGCGCGACAACAACAAGTTGCCGCTGGACCTGCCGGCAGAGACACCCTGGGATTCGCCATCAGTCTGGGCGCCGCTAATTTGGGCCAGAACCGATGCAGTCTGGGCGTTGCATGCGATGAGGACTGTGGCTATGGAACTGGCTGTGATTTTGGATGCGGTCAGGCCCATGGCGTTGATCCCGGAAGCTTTCTAGGTCCGGGAACCATGGGAACTGGTCCATTAATATTGACAACGGGATCAGGAGGCCCACCT

CTACGAGGAGCTCAAGGTGGCGGCTATAAGCAAGTTGGCGCCGGGCAGCGGGGAACTTTTGGAACAGATTTCGGAAGTGAGCAGCTTAACTTCTTCATTACCTTCCTATTGGAGGTCCTCGGTGTTTTAGTGTGAGTATATATTTATATATTCCAGGATTGGATCATTCCAACTCTATGATAATCTACGTAATCTTCCATTAGTTTTTTTGCCATCTGCACGATAACATTTTGGATTACTCTGGGCTACCATGTTGTCCAGTTGCTGGTGGACCTGAAGAACGCCGATCGCAACATTCAGGTGGCGGTGGCCATTGTGTTCGGGCTGCTGCTCGTTGCGTTTGCCATATCGCAGGTGACCAACCTCAGTGGTTCCTGCTGTCATTCACGGGATCGAGCCCGTTCCAGAACCAAAGGTGGTGGCATTGGCTTCTTTCACAGATCCACCAAGCCAAAGACCAAGAGCTGTGCATCCAAAACCAAGACCTGTGCTCATAAGCCGAAGATTAAGAAGGCAGCCACTTGTGGACCCAGCCTGGGCTTTCACACGCACCGCAAAACGAGGTATACGGATTGCGAGGGACGGGCAATTTTCTTGAGCTCGAGACGATATGCTATTCCGACTGAGATGAAACAACCACCCACCATTGTGCTCTGGATGCGCGACACACTGGTTCGCATGTTGCAGTAACCACCAACGAAGCCCCACGTTGGGCGCCAATAATACTTAAACTGACTTAAGACTGCGAAACTATACGTATTGCCTGGAAACACGAACCATTCGATCATTTGCAAAAATTTCAATTTGAATAAACTTTAAGAGAAAACTTTTGTAGGTATTACTAAATTGTTACTTATGATCTGGGCCAGTATTATTAATATCGTGAAATAGATGTTTAAGAATACGAACATACTAGTTCGGTAATAGTATATGTGTATATACTCACTGTTTGTTTTCATTAGTCGCAGCAGTTCGGCAATCATCCAGGTGGCCGCCGCGGGTCCAATCCAGGCCAATCCCTGA

 

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