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CRIMIC CR01596

Gene CG1628

CG1628

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:10600490..10600514	9D4	FBti0210447	86460

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAAGCTGACAACCCTATTGTACCG	CTCTGATGCCTGCGGAGAAGAGATTA

Images

Construct Design Sequences

sgRNA sequence	TAATCTAATCGGACCGCTCGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACGTGAGGATGGGGCTATC	ATCTCCGCGTGGCTTTCG	ACGAGAACAACAACAATCGGTT	CTTCGTCGAGGGTCTGATCG

 

Amplicon 5'

Amplicon 3'

CACGTGAGGATGGGGCTATCAATTAAATTCGCATTGGAACATCAATCAAAGGCGAAACGGAAACGTGCTCATGATCTGCCGACTTGAGGCTGTCAATTGTGGGGCATCATTACATCGGTAAATTTCCACTTGACTGATAGTGGCCCAACCATGTGCATTGGCCGTAGCTGCACGTTCCGCTCCCTGTTTCCCGATGCTTTCCAGCTTGTAGTACTTGTAGTAGTACACCACCAGTATACCCTAGATGTGTACAGATCTATCTCGCACTACGTCACTGCTGTGCGTGGCATGTTTCGAGCTGCGGCTCTATGTGGCTATGAATAGTCTTATCAGTTTCCATAGAAATCCCTAATTCGCTTTTCGCAATCTGCGTAGTAAATACTCAATATTTATGTTTTGGAAAATAACGAGAGGAATGCCAGCCTTCCTCGTGCTCGATTAGATTGGATTGACTGGGCTTTTTATAACCTTAGCTGCTTTTCAATGGCTTATTCTTCCTGTTTATTGCTAAAGCAATTTGGAAAATATTCTAAAAAAAAAGGCCTTTAAAAGCACTATTTTCTAAATATTTAATGTCCCGCAGATTAAGTTTGAGACAAATTAGATCATAAAAAGTTTATAGTTTCGTATGTCAGACAAACAGTAGACCCATTAAACATTTTTGTTACAATTTCTAGGGTATTTAAAGAAAGGCGATCTGCAGTTCTTAAAGATTGCCAACATTGCAACGTTTTCAAGATCGCATTACCCTGGCATTCACAGGCGTCATTTAAGTCCCTGTATATTGAATGCAAAATGAATGCTGAAGTTTTGTAATTGAATGAGCGATAATAAGTTTTTGCCAAGCGATAAGGTCGGCCAAATCGAGCCACTTTTAATCGCCTTAGGTATAATTACCCATCGAAATTATGTTTTAGGATCGTAACTACAATTATGTATCTGAATCGAAAGTTAAATTAAACCGCCATCAGAACGTCGATGGATCCGGCAACACCAAGTGGATCCGATCCCCGACCATGTGGAGTGCTTCATTTGTATGGGTGCGATGCGTATCTGTGTTTATATCGCGCACTGCGATGCGATAATTGTGAATTATTAGGAGGAACAGATCGCCGAAAGCCACGCGGAGAT

ACGAGAACAACAACAATCGGTTTCCCGGTTGCCCAAGGCTACGCCCCCCCCAAAAGATTCCGGGATAAATGTGATCGTTGTGAAACATAGTGTTTCGATATAACCATTTCCACTTAATTTGGGCATTTAGTAGTGCCCTAGTACAGTGAAGACACGCCACAGTGAATTGCAAATAGCTTCTAAGTGGGCATGAAACTAATTATAGATTTTTGGGTTCTCTGGTTGAAGGGAGCATATTAACTTTTTAGACTTCACTTTTAGTTCAGTTGAGTTTATTTATGATGTGCACTGTACCACCTTTAATTCACTCAGATATGCTGACCATTCATAAACTCAAACGAACATCAGAATCATGATCAATTCAATATGCTTGAAAGGTTATATTCGGAATGCTTACTCGGACGTTATATGGCCTTTAATTTAAAGAAAGTTACATATTGTGGTGTAGTTATCAAGAAAATGGACATATAGATACAAAAGTATCTGGATACAAAAGATGCACAGATAGATGCAAATCAAGGACACTCACCTTCGGTCCGCCATATGTAGCGCGTCAGTGTCCAGGGTGTTCGTATGTCCTGCGGATGAGCCGGTTCAACGAAGTTCTTCATCTCGCGCAGCGCCTGCAGCTTACACTTGATCAGCTCCGTGGGGCAGAGGGTCAGTGTGGAGAAGCAAGCGGCCAGGGAACCGGCGCAGGCGTTCTGCACGGTGGTTAAATCGCCAGCCGTCTCCTTGCCCACGCAGAAGGCAACGAATTTCTGGCATCCACCGTAGGCGGCGAACAGCACCGAATTCTCGGCCACATTGGCGAATACGGCCGGAACGGAACCGGCGTACAAGCCGCGCAGTACGCCATCCTTGCGGTATGTGCTCAAAAAGCAGTCCAACATGCCGCGATATGCCTCCGGAAAGGTCTGCAGCTTGACCTTGACCGTGTCGAGGGGCTGGGACACATACACCTGGGCCGCTCCGCCCAGGGATCCCGCCAAAAAGTCGATCAGACCCTCGACGAAG

 

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