• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01597

Gene ATP7

CG1886

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:11857083..11857107	10F2	FBti0210448	86461

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTTGAGGCTAGTGTGCAGGAACCTC	ATATGGCAGAAGTCCAACTTCGCTGG

Images

Construct Design Sequences

sgRNA sequence	TATCTGGAACACTTGGATGAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TACCCGTCGAACAGGAGCTA	TTTACTGCCCACGCACACG	GGGAATTCAATGGCAAGCCG	CATGTGGGCTCGCTGTTTTC

 

Amplicon 5'

Amplicon 3'

TACCCGTCGAACAGGAGCTACTGACCAAATGCTTTCTGCACATCCGAGGCATGACGTGCGCCAGCTGTGTGGCCGCCATCGAGAAGCACTGCAAGAAGATCTACGGCCTGGACAGCATCCTGGTAGCCCTGCTGGCCGCCAAGGCGGAGGTCAAGTTCAATGCCAATGTTGTGACGGCCGAGAATATAGCGAAATCAATCACGGAGCTGGGCTTCCCCACCGAACTCATCGATGAGCCGGACAACGGCGAGGCGGAGGTGGAGTTGGAGATTATGGGCATGACTTGCGCCTCCTGCGTCAACAAGATCGAGAGTCATGTGCTTAAAATAAGGGGCGTGACCACCGCCTCCGTCACACTGCTAACAAAGAGGGGTGAGTGTTTTTTGTGTACTTAAGCTAAGTGGCTTTCTGGAACTAGTTTATCCCGAGAGAATTTCAAATCACGTTCTGGGTCATGCATTCGAATCGAATTGCGAGTCTGGAAGCATTCCACAAGTGGCAGTGCCAATTGTTATGCAATATTTATAATAATAAACACCTGGAATGGGGCCATCAACCGATATGCACCTGTTGCGCTTTATTCTTGCCCAACGAGCACAGGTGTTAAATTCGATCATATATCTTGTATATCTTAAGGGATCGAATAATGCAATGGGAGTAATGAGCTGTAACAGAAGCAAACTATTGGCAATCGTTAATTGATGCAACTTGTGGCAATAACAAATATTAAATAAGCCATTCAAGGGAATTTCGTAGAATGCCCTTATTTGTTTTGCATTTCGCAGAAAGCGACAGCTGTTCCTTTTTATTAACCTACTAGCATCGAGTTCGAGTAGAATCGGTGCAAATGTTTTTTTTTTTTTTATTGTTCGCTTTTCACAATTTCCACAATTGAAAACTGGCCCAGCAATTGTTTATTGCAACTGATATACTCGATTTACTTTATGCTTTTTTTTCTGGGGAGGGTGGCAGGTTCGTTGATAAGAAAGTGCGAGGAGCACTATACTCATATGCATGTATGTATGTATGTACTTTATAAACCATACACTACTAACTATGCTTTCAGCAAACAATGCCAGTGGCAACTAGATTAAATAGCTGGAATTCACCACGCTCGTGTGCGTGGGCAGTAAA

GGGAATTCAATGGCAAGCCGAGAGTCGGGATATTTAAAATATTCAAGATATTCGCTAAAAAACACATAATATAATTATTGTTTTGTTGGGCGCTGGCAGCTCGAGTTTTATTTGCCAGATTATAGCGTGTTTTTTGCGAGTGTTCAGGAAGAACTTATATATACATATTTATGTATGCGATTAGCTGACAAAGAACAAATGAAATGATTTGAGCTCTTTGTTTTCATAAACTTTATACTGCCAATCGCATTTACCAATTAGCATTTAAGCCATGAAACTGAAAGTCCGCCTGGCCAAAGAAAAATCAAAACAAAAGTGAAAGAGCTCCAATACGCCTTGAAATACAAGTACATACATATAATGTATGTACATATATATGTATGAATGTATAATAATTTATATACCCGCGATTGGCGGGCAAACGAGCGAGGAACTAATCTCGCTTTTGTCTGGAAAAAAAGAGGGAAACCTCTCTCTTTTTCAGCTTTGCAGTCTTTCTGCTCCGCATGCGTGGCCACGAACTCCACTTCCACTTCGTACACAGCGAGAAATGGGGCTAACTATGATATAAGTATTATATAGAAGAGTGCTATCTACTTCGGCATATGATCAGCATATAAAATCTACTAATATTTAAAAGCACGAATCGTACAATGTTTAGCAAGTTTCTTTTATTTTATGTTAAGCACGCACTTTTTCTTCGAGTGCATTTCTAGTTTCATTCACCTTCACTTTGATTTAGAGCTGTTTTTACTTTACTTTTGCTTTGCCAGCTCAAAAATGTTGATTTGAAATTAGTGAACTAGTGGCGAATTATGCAATAACTTGGCTTTGATCCACCACATTCACATTGAATTCTGGCTGATTTACGAATATCGGTTTGCATACCCGTATTTATGTATGTACTAGATGTACGTGCGCCGTTTTGAGAAGCTCAATTGCAAATTCAAAAATGAAAATGCATAAATGGGGAGCCTCGTAATTATGATTGCAATGGAACTGGTTTTCGATTTTTTCGCATTCGACATGGAGACATGCGCACGGTGGAGAAAAGCGAGCGAAACTGTTGCGATTAAATGACACTGAAAATAATTTCAGACATTCAAAAGTGGTATGAAAACAGCGAGCCCACATG

 

---

Search Again