CR01598

CRIMIC CR01598

Gene CG9095

CG9095

Information

Donor Location Cytology FBti Stock number
pM37-p1X:15155975..1515599513B1Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band CCAAGCTTTGATTTGTGGCTGCCCAT GAAAGTGCAAGTGCTAGTACCCGATG

Images

Construct Design Sequences

sgRNA sequence TCTTCAGAGGGGGTGTCTTCGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TCTCACGCAGCTTCTTCTCC GGGTGAAAGATGCTGCCTTG ATCGCTAGATCGGTGGGTCT CTCCGGCTTTGTGTTCTCCT
 
Amplicon 5' Amplicon 3'
TCTCACGCAGCTTCTTCTCCTTTGTGGCGCGCTTCTCTTGACCCAGTTTGCTAAGCTTTTTGGTGTTGCTGTTTGCTGTTATTTTTTTTTTTGTTTTTTTTTTTGGTAAGCCAAATTAGTAGAACCTCGTTAAAATCGCACACAAACAAACCGACACACACTCGGAGAGTTGGAGAACATCGAAAAAAGATTTCGCAAGTGAGTCGACTTGCGCAATGTGGCCATTGTTGCTTTTGTTGGCGGATGCGCCTGGCCAGACGAGACTTGTGCAAGCTGCAAGTTCGTTGCCTAAGTCTTTTGATTTGAACTTCCGCCAGAGGATCTCACTTGGGCGACTTTTGGGCACTTATTGCTTGATGAGCACTAAGCGAAAATTTTGTGCCATATATATCATCTATAAAGTTAATTATAAAGTCACCTCTTGACACTTACAGGAGAAAAGCAATTATAGAGAGCAACGTAAATTTGAAGTATAGCTTATAGCATGAAGTATAGCATAATTCTAAGATCGCATTTGGTATTTCAGTAAATGACAAAATGTATGATGATAACACATATAATTAGGTTAGTATAAAGTCAAAGTCCCTAAGCTATTATTAAACCCAAACATATTTAATTAAGCAGGCCATGGTTTTTTTTTTCGGTGTCTCTGTCCGGGGGACTGCCCTTGGCTTTTGGTATTCCTCGCCAGCCGTTCGTTCTTGCCTTTTGGAATTAATTGCGCATAAAAATTGCCCATCATGCGCAGTAAATGAGACATCAAAGAACGTCTGCGAGCTAGTTGGCTGGCTAAGAGCTGCTAAGCGTATTAAATAAACATGCCAGCGATGGTTTCCCCCCCCCCCCCCCACCACTGCCACCGCTCTTTTATGTAACGGTACGCACAGCAACAATAAATTTCAAGCGTAAACAGTTACGTAAAACAACAAGAAGAACAACAGCAACGGCGGCAGTGGTTGAACAACCAGAAGAATTGCTGCTGGCTGTCTTCTGCTCACCACAATGACAGCATCCATTCCATACCATTCCGTTGGCAAGGCAGCATCTTTCACCC ATCGCTAGATCGGTGGGTCTAACTCGTAGCTTTATAACAGGTTCGATATGATTTTGCGGCACTTTTGCCGCAAAGGAAACGCTTGATTGCCGCTTCTAAACCAAGGCCAAGCAACGGCAATCGTCAGCGGACCATCGGCGCAAGCCAAAAAGACGATCGACGACATTGACCGACGATGCTGAATGCTGAACACTGGACTCTAGACTCTAGACTCTAGACTATAGACTCTAGACCATAGATACTGGATACTGGATGCTAGGTGGTGGTAGCTGGATGTTGGAAGCTGTAAGCCGTCTTTGCCGTTTGCCAGTTGACCGCATTCCAAGCACCAGCCAGCAAATCAATGTGCAAGCGTAAAAGCAACATCATCAGCCCAGTTGCGGTTGTCCTTCTCCTCGCTGCACAAAACAAAATCATCGTATTGTACTTAACATTATATGTGTATAATATTATACAACTGAAAGGCAAATTTAACAAAAAATTATTAGTTACAGCTAGTGCAACTAAATATCAAAATATTCTCAAATTTTTTCTATAAAAAAGAGAGCAAAAGCATGCCCAATTTGTTTCACTGTGCGCTGGGTTGCAAACTGGGTCACTTCGGCCCAAATGCAGTTTAAGATAGAATGCCAGCTGGGTGTGTGTGTGTGTGTGTTTGCCAGCTGCGAAACACCTGCAGTTGCAGCAGCAGCAGAAAAAGCAACTGCAGCAGCAACATCGCCGCTTTTCAGCTCGTGCCACAGTCAAGACTTCACAGTGAGTGATTGCAGTGGGGAAATGTGGTGAGCGGTGGCACTTAGCCGGCGCCCACTGTGCACTTTAGATGCGTTCTCATTGCAGTTGCTAATTTCTGTGGCTTTTGCATCGGCTTTTGGTTTTGTGCCTAATTTGCTTTGGCTGTGAACGTTCTCAATGCTATGCGAAGATTGCCCCCACTTTGGTTTTATGGGCTTTTTTTTTTGGGTTTTTGTTTTGGGCTTGTGGCCTGTGTCTGCCACCAAAAGTGGACAAACGCTGGGGTTAATCTTTTGGTCAGACCGCTTCCAAAAACACACCACACACACACACACCAGTGCAGGCAAGTAGTTAAAAAGAAATGGAAATGGTAATAGAAATAGAATAAAAAACAACAGTTTACAGGGCAATTTGCATAAGAAAGGAGAACACAAAGCCGGAG
 

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