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CRIMIC CR01599

Gene HERC2

CG11734

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:20397728..20397768	19C6	FBti0210449	86462

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCATTTCTCACCTGGACCGCATGGT	GGATTCGAGTCTTAAGGCTGCAGAGA

Images

Construct Design Sequences

sgRNA sequence	TGCCAAGGCGCGGTCGCTGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCCAGGAGGGTAAGCTGTA	GTGATTCCTGGTGCCTCG	CTATGCCGTGAGCTCGACC	GGAGGCTGGACAGATTTGCT

 

Amplicon 5'

Amplicon 3'

AGCCAGGAGGGTAAGCTGTACACCTGGCGCCTGGCCAAACCGGAAGCGGAACCGCTACCCATGGAGGAGGTCGCCCACGATGTGTTCATCAGCATCGCTGGCCACTGTGAAGGTCGTCACTTTCTGGCCATTGACAGCAACCGTAAGTGAATTTCTAAATTGAAAAAAATTATCATTGCTGTTCGTCTTCACTGCTAGTTCGTTTTACAGCCATTATATTGGTTATCGTACATATTATATTAAAATGTTGGGACTTCCAGAGATTTAATAAAAAGTGTAGCACGCTTTTCAGCGTATTTTTTTTTATTATTGAACTTACGGTAGGAAAATTAAAATCTTCTAAAGATACCACCTACCGTCCGTAGGTGGCATGCACGATTCATAATCCCTAGTATAATTATTCCTATCTGCTAAAACAAAAACCTCCGTGTGCTCTTCAGTCCTTGCACGCTCACCGGGACTGTCAGTCAAGGTATGACTTCGATGAGCAGGATGTGAAGCCAGGTCACAGCAAAGCCTTTGCTACCGCCACCAGCAGATTCGTGGCCGGATATATCCACCGACGTTGGGCATGAGTCTAGCCAATGAAGAAGCTGTTATTGCTGCCATCTTGCTGGCGTAACTTGCGTGGTCCTTGAACGATAGTCTGGGGTCTATCATAACACCCAAGTACTTTAGGGACTCTTGGGACAGAGACTAGCATATTCTCCACCTTTTTCCTACTTCCTGCTTAGTAGAACTGCCCGTTAATGCTCTCGGTGATTGCCTCTATACGAGTATTAAGGATATGTTCGGACAGTTTTCCAACTATATCCAGTAGGTAAAGAGGGCGGTAGCTGTTTGCAGCATGTGCTGGTCCATTTCGGCAACAGGACTAGGTTCTGGCTTTTCCGCCTCGTTGAAAAGATTCCGTCCAAAAGGCATTGCCTCGACGACTTCGAAATCTGTGACGCACGGGAAATCTGAGGCAGGGGCTACTTCTGTTGGTCGTCATAGTGTGGTCTGCATTGGTGTGGTCTGCAATATTAGCCAGGACCCCAGTTTCAGATGGGGTTACATCTGCTTATAGGCGAGGCACCAGGAATCAC

CTATGCCGTGAGCTCGACCTGGTGGGTACTGCCTCTTCCTGGTGTCCCATGCTGGCCCGGCTGTTCTTCTCTGCCGTCTCCAAGGTTCGCTTAAGAGCACGATGTCTATATTGCGCTTAGCCTCAGTCTGACCTAGGAGTATGATCTGAGCTGGATTACGTTAGTATGGGCGTGCATTATAGCTTATTTTAGGCTCGTTCTGTATGTCGGGCACGCGAAGGTACCCGTCGAGTGGTTTCTATCCGCCTCGCTGCTGCAGATCAAGCATTTTGGTTGGTGAAGGCTGTGTGTGTTAACTTAACTTTAGAATAATAGGAGTTATAAACCTGATGCCCTTTTTTCCAATTCGTTGCAGACAACGCCTACTCCTGGGGCACCGGCGAAGATCACCGCCTGGGTCATGGCGACACCCACGCCCGAGCCGTTCCCACCAAGATCGCTGCCCTGGAGCAGCACTGTGTACAGTCGGTCTACTGCGGCTGCTCGTACAGCGCAGCCATCACCTGCGGCGGCAACCTGCTGACCTGGGGACGCGGCACCTATGCTCGGCTGGGCCACGGGAACAGCGACGATCGCAGCCTGCCCACCCTGGTGGTGGCCCTATCCGACCACATGGTGGTTGACGTGGCCCTGGGCAGCGGCGACGCCCACTCGCTGGCCCTCACATCCGAGGGACTGGTGTTCGCCTGGGGCGACGGTGACTACGGCAAGCTGGGCAATGGCAACTGCAACGGCAGCCTGCAGCCGATCCTTGTGGAATCGCTACCCCGGGTGCAGCGCGTCTTTGCGGGCTCCCAGTTCTCGGTGGCCCTGAGCAGCGAGGGTCAGCTGTATACGTGGGGCAAGGCCACCTGTTTGGGCCACCAGTTGGTGGAGCGCAGTGTCCAAGGATGCAGTGTTCCGCGTCTGGTTAGCAGCTTGCAGGTAAGCTACCGCCAGCTACCCCGTTATCCTCACATCCTGTCATAACTTTTGGTTTTTCCTGCAGCACAAACGCATTGTGGACGTGGCCGTGAGCGTGGCACATTGCCTGGCGCTCTCTAGCAGCGGGGAGGTATTCGGATGGGGTCGCAACGATAGCCAGCAAATCTGTCCAGCCTCC

 

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