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CRIMIC CR01607

Gene dock

CG3727

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:830668..830686	21E2	FBti0210451	86464

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCGAGCAACTGCAACACATAACCA	TGAGCTGCCTACAAGTTAGCCTTGGT

Images

Construct Design Sequences

sgRNA sequence	TTATGGTTAGATTTCATTGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTACCAGTACCGTCCCCAG	TGGCGGTTTAAAGTGGGTTAGA	AACTTCATCCCAATACTGATCGT	TTCGCCCTGCTTATTCGTGT

 

Amplicon 5'

Amplicon 3'

TCTACCAGTACCGTCCCCAGGTGCCCGTGCCCGTTCCCGGCTCACCTGCCCATCACCAGCGGCACTCTTCGCTCTCCCAGCAACAGCAGTTGCACCACCACCGCACCCTGTCCACCGCCTCCTCCTGCAGCGCCCAGCACAAAAGTGTGACCTTCGGCCAGCCAGCGCTCGACTGCGGCGGCAACGGCAGCGGAAGCGCCAACGGAAGCGGTAGCGGCAACTCCTCCAGTGGCAGTGCCGCCGGCAACGCTGGAACCCAATCAATGGCGGGCAACATGAAGCACGGTGAGCAACTGCAACACAATAACCTCACAGCGAGAAGAGCATTGGGTGTAGTAAAAGGATCTAGGATTTGTATGCTAATGAATTTGTGGTTCTATCATACTGGCCCATTTTGCTTTTCTAACTGTGTGTGCACTTGGTACTCTTCTAACCTGTTCCTGTTAATCACTCCGCAGGCAAATCTCAGGACGACGTCTGCTACGTGGTAGCCAAGTACGACTATGCGGCGCAGGGTGCCCAGGAGCTGGATCTGCGCAAGAACGAGCGCTACCTGCTGCTGGACGACTCCAAGCACTGGTGGCGCGTCCAGAACAGTCGCAACCAGTCCGGCTACGTGCCAAGCAACTATGTGAAGAAGGAGAAGCCCTCGCTCTTCGACAGGTGCGTCAGATTCCTGGCCCGACTAAACCTAAGCGTTCTTCTTGCTCTGATTTCAGCAGAATTAAGCCCTCTATACCTGTATCTGTATCCGTATCCGTAGATGTATCTGTATCTCTATCGCGTTCAAATACCCGCCTCCACCTTTTGCTTTGGCTATCGTAATTTAACTTATAACTGCTTTCGGCTTTTGTATTTTGTATTTCAAAACAACAAATTCGTTTTGATTTCGTTGTCTGTCGAGCCCCACTTGCCCCTAAAAACTAAAGATCCACTAACACCCAAACCAACTAGGGCTAGTCTAACCCACTTTAAACCGCCA

AACTTCATCCCAATACTGATCGTGAATATTTTGATTTACGATTTCTGTGCAGCATTAAGAAGAAGGTGAAAAAGGGCTCCGGATCGAAGACACTGCCTAATTGTTCGCCTTCGCGACAGGTCGAGAGTCCAACCATGTCGCGCCGACTTCCCCCAGATCCCGCAGAGGCCATCGGAACGGCTGTGGTCAAGTATAACTATCAGGCCCAGCAGCCCGATGAACTTTCGCTGACCAAGGGCACACGAATCCTCATCCTGGAGAAGTCCAACGATGGATGGTGGCGTGGACAGAGCGGCAATTCCGTCGGCTGGTTCCCCAGCAACTACACAACGGAGGATTGTGATAACGACGGCGAGATCCACACCTACGCAATGGCCGAGAACGTGCTTGATATTGTGGTGAGTAACTGGACGTGTTCTCAAAATAAACATTCACATCATCTATGAGAGGTTGTCATTATTGGCTAATAACAACTATTTTCAAATATATAGGTCGCGCTGTACAGCTTCACGTCAAACAACGACCAGGAGCTCTCGTTCGAGAAGGGCGATCGCTTGGAGATCGTGGATCGGCCCGCCTCCGATCCGGACTGGTACAAAGCTCGCAACAATCAGGGCCAAGTCGGTTTAGTTCCACGCAATTATCTCCAAGAGTTGAACGACTACCTGGCCACGCCCTATCGTAATGCGAGTGCCAGTGCAGGAAACGGAAATGGAGGAGGAAGTAATGGCGGCGCAGGTGGTGGCGGAGGAAACGACTCCATGGAGCGGCGCAATGAGGGTAATAAGCCGGCGGCGCAATCTTCCGGCCAGCCAATCGAGCGGCCAAATCTGGCGGGCAAGTCGTGGTATTATGGAGCCATCACCCGCAGCCAGTGCGACACGGTGCTCAACGGCCACGGGCACGATGGCGACTTCCTCATCAGAGACAGTGAGACTAACGTAAGTGGAATTCGCAATTGAAGTGCCATAACATAGTTCCGCCCGAATTCTTTGAGAGTGTATAAACGAATTCATCCTTTCTTGCAGATGGGAGACTATTCGGTTTCGTTAAAGGCCCCAGGGCGCAACAAGCACTTCCGCGTGCACGTGGAGCAGAACATGTACTGCATTGGGCAGAGGAAGTTCCACTCGCTGGACCAACTGGTCGATCACTACCAAAGAGCGCCCATCTACACGAATAAGCAGGGCGAA

 

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