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CRIMIC CR01609

Gene CG1407

CG1407

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:10031874..10031885	46D6	FBti0210452	86465

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAGTGGGTGTACGAGGAAATCTGTC	GGGCGGACAATTGGTTACTAAGCTTG

Images

Construct Design Sequences

sgRNA sequence	CAGCAGCAGAACAATTAGGACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTGAGAGAGCTTCTGGTGGA	GGCGCATTCCGAAGCATTTT	TTCTGCTGCTGAGAAATGCA	CCGCCCATCCCTAAACCAAT

 

Amplicon 5'

Amplicon 3'

AGTGAGAGAGCTTCTGGTGGAACTAGTAGAAATGGTGTAGTAAATGAATCGTCAAATACCTCGAAAACACGGGCAGGAACCAGTATTGCCAGTCGTCACCAAATACTTCGCAGAAATTGGCATAACGCCCCAAGTTATAACCATTCTTATCGGGGCCGCCAACGCGGAAGATCGGAGCCCTGAACGACTCTGAATGGAAGATAGATTAAAGAGGCTATGGTATGCATGTGATTTATAAGTCAGGCTTTAGGTACTTACCCAATGTCGTGCGATTCACCAGCACAAGGTAGATGTGGTAGCCAAATAAACTCACCAAACTAATGGCAAACATAATGGCAATGAAGAACAGGAACAAGATGTGAAAACGACCCATTCCACTGGCATTTAGCTGTCCCTGTGCCGAGTAACCATTATTATCGTACTTGGATCAGTAAGTACAAGCAGTTTAGATAACATTACAGCAAAACGAGAGAATACATTCATTAACTAACAGGTCTTCGCATGCAGCGGGCATTTAGGGTGGTAGCAAGAGTAGAAACGAATCGGAAACTTACTGCACCTACCTTCCAGAACTCGACAAAGTCGTGTAGCGAGGTAAAAGCCACATACAGGCAGTAGACAAGAGCATAGCCCAGGAACAACACGAAGTACTTGTAGTTGTAAAAGTTAACGCAGTTGTTTACCCAGGGGCAGTGGTGATCCATCTTCAGTACGCAGCAACTGCACACACTACAGTGGTGAGCTCGGTCTGGCTTGATGATCTTGCACTTTTCGCAGAACCGCACCGAACCATTCATTGTGCTGCAAGGCAAAGGTTTAAATATTATCAAAAATGCTTCGGAATGCGCC

TTCTGCTGCTGAGAAATGCATTTGGAAATGTCTTAATTGGGACAACTTTTAATTTCTTAGATAAAATGGTTTTGTTTGATAAAGTTATACAAATACAAATCAACTGACAAGATATAACATAACATAACGCTTACCGATTCGTTACTGGCAAATCGCGCGCAAAGTTGTTAAGAATGCGTTTCTGGGTGTCCGGGCTGTCGGCTCGGAAAAGTCGCGACACTTCCTCATCCGGTATCCTCCACTAAAAACGGGAGAAAAGCAATCGAATATTGTAGGCTTTTCCGTTTTCTTTTAATAACACGAGCTGATAAGGTGTATGATTCACTCACTTGATCTGGTATTCGGCCTACGGAGGTCATTATGGTGCGCCAGTACGACCACATGAACAGGGTGAGGAAGAGGTGGTAGAACAGAAGCATGAAGATCATACCAATGCGGTTCTCCGAGTTGCCTGAAAAAGGAAACATCAGGTTATATCCCCCCCCTACATGACTGTAAATACAACTCACGGATGCATAGCTCCACCACATAAGCGTAATAGGACCAAGCTATTACCGCGGTGATAAATAGCACTGGTATCCATTTGAAGACTGCCATACAAAATCCGCAGGGTGTCTTCCGCCGTCGATGATCGTCGTTACCCATTCTATTTATGTGGGCCACCACTTCGGCAAGGGCGCGTGTGTCTCGGGGCGTTGGTGTCACCTCCACCTGGAGCTGGAGCGGATTAGGGGCGATCGATGGGTCTGCAATTGTGGGTGCTCCTCCAGCGGTCGCTCCACTGGCCAATTCAATGTCTTTATCTTTTAGTGATTCACTGACTTCGCAAGCTGTTAACAAACGCTTTTTGACGCTATCCGCTGCTTGCTTCCCCTTTTGGGCGGACAGGTGCCTATTTCACCTTATCGCTGCGACATTTTCGGTAGTATTTCTCAGTTAATTTGCTTTTTCAGATGAAACCAACTAAATTTTGGCTGTCAAATTGGGGAAACTCAAATTGGTTTAGGGATGGGCGG

 

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