• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01610

Gene Sox15

CG8404

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:14207777..14207796	50E4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	AGGCAGTCAACACCCTGTAAAACGTC	ATTGTGATTCATGTGACGCTCTGCCG

Images

Construct Design Sequences

sgRNA sequence	CCTATGCCCCAACCTCCAACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGGATACTCTGAGTTGCGG	AAGTCGTGCCAAGTCGTTTG	AGGTTGGGGCATAGGTAGGG	CAGCACTGCCTCCATTTCCA

 

Amplicon 5'

Amplicon 3'

GGGGATACTCTGAGTTGCGGACAGGTATGTGACAACAATTGGGCAGATTTGTCTCGGATTCAGAACAAAACAGAGTGAAGAACAATGGCATTCTATTATTCGGGAGATTTTGTTGCTGGGTATGTCATAGAACGATTTAGACACTGATATGGAAATCAGTGCATATTTCGAAATTATCGTGTAACTGGCTTATCTGAAAATTATGGAATTATAATAGTGAGTTCTAATTACTGCGCTTTCTAATCTGATCTAGTTATCCATAAGATTAACATGTTGGAAATGGTTATTTTCCTATTAAATTTAATTATATACCAATTTCAATTATGCATGATTAGGCTCTAGTGCCAGCGTTCCAATTGAGATAAATCCCATTGGTAATCCGATTAGCTTCATATACATTTTAGAAAGCTTCTGCCCAGCAAAATGGTAATGAACAACCTTGATATATAATTTATGTAGATCTGATTGCGAGCTCCGCTTAGAATCCAATCTAATGCTCGATTGCATAGTTAGGCATCCGAGATTTGCCCACCAAAGAGATGCATGCCTAGAATGGCAAATCATTTGGCGCCTCAACTGCGATCTCGTATTGGGCCAGTTTTGCAGCCTGGCAGCCTGTGGAGAAATGCAAGCGGTGCGGCAAGGTAATAAAAGCCATTTTGGTTTTCGGCCACAGTCAAAGCCGGCTCGGCTCGACTGGCGAGGCTGAATCTGGATAAAGTGGCGGAGTAGGAGGTGGCTGGTGGGACCTGGAGGTGGGTGTGGCCACAGCGCCTGGCGAAGAGGCATCTGCCGTGGCTCTCCATTTGCTCCATTTGCAGTCTATTGGCTGCCTGCTGCGGCTGCAGGCGCCCCGCATACAAAACAGATTTCCAGAGGCCCAGCACAGCACAGTGCACAGTGGAGCTCAGCGCAGGCCAAGAAAACGAGTTTGGCCCAAACGACTTGGCACGACTT

AGGTTGGGGCATAGGTAGGGGCGCTGGTGTATGCCAGCATGGATAAACTTGTGGAGCTGGAGCTGCGGCCCAGGTGTGATGTACTTGAATAACTTAACGACGCAGGCCCGGTCTTTCAGCGGGCCGCGATGAGTTGTCGGTCGGTCGGTCGGTCGATCGGATCGGCCATTTACGCATACATATGCATGGCCGCCTATATAATCGTACGTATATGCATATATCTGTATGGCCGCATGTAGATGCTGTTGACCAATAAGCATAAACATCCCAGCATCCGGGCCTAAAGAGCCGCCATCTTAGCTTTAATGAACATGAACATGAAGGTGAGCTACCTGCCTCGCTGCGAATGGGAATGAGATCGGTACGCACAATGGATTGGGCTATAAGGTATAGGTACCTCCATACCTGTGCCTTCTATATAGAGGCGATATATGTGTGTATGTATGTGTGACCTGGCCTGGGCATACCTAATTAAACTAATGAAGCAAGCGAAGAGAGTAGCGTCATCATCATCATCACCATCCATCTTCGAGGGCCGAACGTTGCCGAAGCTGCTGCTTTCGTTCGGCTAATGAGGTTTATTGCGGCTGTCGTCGCTGTGGATCCCCCCAGCCCCACTTCTTGAGGCACCACCCACCCCCATGCCATGCCGAAGAAACAACGAAGACCCAGTTCTCACACAAGCGATTTTAGGAGCATAAAAAAGATACAAAAATTTAATGGATTTTAGCACGCACACCAGCACACGCAGCCAACAGATACATGTACGTGCTCAGATACAGATACACATACAGATACTTATACGTATACACCCTCGCAGGTGGAGGGGTACTAAATTATATGGATTGCATGGAAATGGAGGCAGTGCTG

 

---

Search Again