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CRIMIC CR01611

Gene gol

CG2679

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:25075940..25075951	60F3	FBti0210453	86466

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCGATCATCGAGGGAAGACGAGGT	AACATTGGACTCAACGGACGGGTTTG

Images

Construct Design Sequences

sgRNA sequence	CCTGCCTTACCGACGTATTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TACTCATGTTGCTGGCCTGG	ACGTCGGTAAGGCAGGTATT	AGCAGTTTTTCTTGGCCTGC	CTACGTCCCCATAGAACCGC

 

Amplicon 5'

Amplicon 3'

TACTCATGTTGCTGGCCTGGAGCGGCTGCAAAGGCTGCGGCCCGTGCGGCACGGCCACCACAATGACGGGCACCTCCTCTATGGAGGCGATTGGCGCTGTATGCTGTGGACTCGGTGTTTGATAGATTTGGTCACCCACCACATATCCATAGAACTTCAGGACGTCGAGCTTACACATGGGACAGGTTCGATGCTCTATAAGCCACGGATCTATGCAGTTTTTATGGAACTCGTGCCTAAAATTAAGAAGTTCAAAGGTATTTCTTTTGAAAGCTTGCAGAGCTGCATTCTTACTTGCACGGCAATATCCGTATGGTGTCCGTGGGCTTATAGGCTTCGATGCATATAGCACAGCAGTCGCTATCCAAGTCCTTTTCATCGCTGAATTTTCCGGTTTTTGTTGGAATCTTCATGATAGCCTTTTTGGTAACAGAGCAGAGATTGCGCTACATCGGGCAAGGATGAATTAAATTATGTATTTGATTATTAAGTGAAGGCTTACCGATTGTTGGTCCTTCGCCTGCATGTAACGGAACCTCTGAATATAGTAGAAGATGAGCCAAACCAGAGAAATAATCATCAAAACAATAAATGAAATCGACACAAACAGCACTGATGTTCTAAAATATAAATGGAAAAACAGGTAATTATTTAACTTAAGTTCGTTAAGTTCAGAATATACCAAAACTAAGACTAAAGCTTATAAGTCTTTACAAATATCCAAAACATATTAAGATCCAAAGACTGCCTGTTCGAATGACTAGTTTTCAAACTAGAACTAGAACTAATTAGAATATTAAAATTTTGAAAATAGAAAAAATACCTGCCTTACCGACGT

AGCAGTTTTTCTTGGCCTGCCTGTAGTAATGGTTTAGGCAGCACTCATGACCAAGGGGACGTAGCTCAGTGGTAGAGCGCTCGCTTCGCATGTGAGAAGTCCCGGGTTCAAACCCCGGCGTCTCCACATGAATCACCTCTTTTTTGTTATTGAACAAATTATTCACGGCAAGCGACCGCATTGGTTTTATTTGCTTGTTTAACGTAAAACAGTTACATTTTGACGTTACATGATGTAAAATTTTAGAACAGGCTGCATGATTGGGCAAATGGAATTTAATGCACTGAATTACCGGCGTTCTTTTAAGCAGTCAAGGGTCAGGATCAAATCAGAAACCAAATATCAGAAGTATTTAACGTAACAGTTACATTACATTTTGACAGTACCATTTAACTCAGTCACAGTTTGGGGTATTATTATGCACACTTTTAGAAAAGATTTCAAGATTTGATTAACGAAATAAAAAGCAATCAGGATCCGGATCAAACCAAAAACCAAATATCAGAAATATGTAACGTAACAGTTACATTAAATTTTGATTTCACATTTTAGAAAGGCTGCAAGATTTGACAAAGGGAATAGAACGCACTGCATTACTGGTGTTTTATTAAGCAGTCATGATTCGGATCAAATCAGAAACCAAATATCAGACGTGTTATACGGAACCGTTACATTACATTTTGATGTTACATTTTAGAACAGGCTGCAAGATTTGACAAATGAAATTGAAACCTGCTTTACCGGCGTTCTATTAAGCAGTTTAAGCGAAAGTGGTTCTGATTCTAGTTGATAATTGGTCACCAGGAAGACGCAAGGGGACGTAGCTCAGTGGTAGAGCGCTCGCTTCGCATGTGAGAAGTCCCGGGTTCAAACCCCGGCGTCTCCAACAGAGTTGTCAATTATTTTTTTGGACTAAGTTTCAAATGTTGGTTGGATTTAATCGTCCTCAGAAAACCAAATGCATTGCAAGTCTACCGAAGCAATTGGGAATTGGAAAGGACTTGAACAATTGTTTTGTCAATTTCGAATTTGAACTTTTTTGGACTAAGTTTCAAGTGGGAAATATAACAATTACTCTGATAATAATGCGGTTCTATGGGGACGTAG

 

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