• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01612

Gene Cib2

CG9236

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:20880295..20880312	57B4	FBti0210454	86467

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTGTCCTGGATTTGGATCCCAAGC	GCCATTTCGGCCATCGTAAAGTCCAA

Images

Construct Design Sequences

sgRNA sequence	GCCTCCCATTGTTTGCCTCTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTCGTGCGAAAGGTTTGGC	GGAGGCTGCAAACAATGGTG	GCGGCAACAAAAAGCGGAA	TCACTGACCGCAACAACTGA

 

Amplicon 5'

Amplicon 3'

ATTCGTGCGAAAGGTTTGGCTATTAGGTGCTTAAAGTGTAAACTGACAGACATTCTGGCTAAGATCTGCCAGGTTCAAGTCTTAAATCTGCTTAATTGGCCAAAAGCGGAGTTAAATCCCAAGTCTTACTGATGTCTTGCAACTGGAAATACAAGAAGTTAGTTAACAATTGAATATCTTTTCTTATTTATTTATGAACACATTTTATATTGATCCTCGGAGTTCATGTAAATCAGACAATGAATTACCCGTGCCATTATCAGGTACTTTCCATTCGCCAGACCGATAGAGTTAGCTTAGTTTTCCATATTTTTCTACCGACTTTATAGGGGCGCACCTCTAAAAGCGGCTTTTGCCGCAGCTTGACAGCTAAAAAGAGCAAAAAACCACAGGATACACGGCGCTGACCTGCGAAAGCTACATGGTAACTGTATAGTTATGGGCCCAATGGGGGTGTGGAAGTGGGAATGGTAGTGGTAGTGGGAGTGGGAGTGGCGCTCCTGAACACTCCTTAATTAATTTCCATGCGAATGCGGGCGCCATACGCTGGCCATTCGCCACAGGATGTGTACTAAGCCGTGGAGCCGTGGAGTCCTGGTGCTATGCAAAGTTATGGCGCTGCGAGTTGGCCCACAGTCGGCAACAGTGTCAGTTGCCATTCACGCTTGGGACTAACCGGTTTGGCCGGCAGCTACGCAAAAAAAGCAAACCAATCCAAACGGATTCGAGCCAAAACGAACCGAACCCAACTGGCTGGCGAGTAACGATGGGCAACAAAGTTGTGACATTTACGGAACAGGAATTGGATGACTACCAGGTGAGCGATCAATCAGATGAAGTTCACTTCCACACAGCTAATTTCTCTTCATGCATTGCTGGTCCGCAAATCGGAAAGGATTGCACGTTCTTCACGCGCAAGGAGATCCTGCGGTAGGCCATTTGTATTCCCTTAATAAGACAAAACTATTCCATTTCATATGGCGGCAGACAAAGCACCATTGTTTGCAGCCTCC

GCGGCAACAAAAAGCGGAAAACACAAGAAAACACCCACACACACACACACACTCACACGACAGACACTCAGAACACAAAGCCCATCATATCCCCGCCCTTTGACCCGGCCAACACATCACATTCCATACCCATTCCCAGGGATTATCATTTTGGCCAGTATCCACACTATTGATCAGCAGCCTCGAAAAAGTCAGCCTACTTTTAAAAACCAAATATAGTTAGAGTAAATTTAATATAATATTTGCAAGCTCGATGAAAAATTCATTTTTAAGTTTCTGAATGAAAAGTTAAGTTAACTGAAGTTACTCATAGTGTATTTTATAAATACTTTAGGTGGATGCTGTAAAATTGCTTTCCAGTTAGTCTAACACATTTCCAACTGGCCAGGGTATCCTTAACTTCGGTCACCGAAGACAATCCTTTATCCTTGTATTGCCTTTCGTTACAGGGTTCACAAGCGATTCCGTGAGCTGCGGCCGGATCTGGTGCCTCGCCAAATGACCGAAGGTCAGGCCTCCAGTGTTAAGGTGCCCTGCGAATGCATTGAGAAGATGCCCGAGTTGCGTGAGAATCCCTTTCGCCGGCGAATATGTGCGTAACAATAGCCCCGAATGGCTGCATAAGCAAAGCTCAGCAATTCAATGCCAAAAAAAAACCCAGCCAAAAGGCGTGTACCTCGGACGCTGGACTACAGCAATTTTCCACTTTTTTGCAGGCGAGGCCTTCTCACGCGATGGCCAGGGCAATTTATCCTTTGAGGACTTTCTGGACGCCCTCTCCGTTTTTAGCGAGCAGGCGCCGAGGGACATTAAAGTTTTCTACGCTTTCAAAATTTATGGTAAGTTCGCGTTAGCTCCGCTCTCAGTTGTTGCGGTCAGTGA

 

---

Search Again