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CRIMIC CR01613

Gene Dhit

CG5036

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:17796638..17796651	54E9	FBti0210455	86468

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTGGCAACAATGGGAATGGCAATGG	TGCTTAGTCGATTGCAGCAGCGATTG

Images

Construct Design Sequences

sgRNA sequence	TACTCCGTTGCTCTGTCTCCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCTTTCTCGTTACCGCGCT	ACGGAGTACTCGAACTTGACA	GGGCTTCGAAACCCAAAACC	AACCGGCATTCGAGTTGTCT

 

Amplicon 5'

Amplicon 3'

TTCTTTCTCGTTACCGCGCTGCCTTGACTCAACCGTTCGGTCATTAAAACAACTACCTGTGCCAATACCCATCCACTGCCAACCACATCCTGACAACAGGGCCAAATGGTAAGCACCTCCTGAAACACTCATGCATTCATACACCGAGAGAATCGATTGGTATTATTGGACTGTAAAGTATAGAATATTAAATGCGCAATTATTTTAAATACGTAATTATTTTACCTAGTTAGCTTTATTTTCAAGATATACTATAACAGATGAACCAATCTTTGGTTACATTAACATCGTTATTGTTAATTAATTTATTTATTTTTACTTATTTCAATATCAATTTTTTGCAAATTTTTTATTTTTTTATTTTATAAATCATTTCATTTTAAAGGTTGTCTGTTTGAGCCCTTTTTTTAAGAGAGTCGAAATATTATTAGTCCATGTTTCTCGCAGTGCTGTCATGGATGTGGCCACGGTTACAGCTATGGTTATTGCTTTGGCTTTAACTATTGCTTGGCTTTGGCTTTGGCTTCGGCTATGGCTGTGCCCACTGACATGGATATGGATATGGATATGGATAGGGAAGTTGAAATGGAAATGGACACGGTAAAGGTGGCCTTCTGCTGGCAGCTGGCAACGCTGCTGAACATCCAAAAGCTCTTTCTCTCTCCGCCATGCGAACTTTCTCCGCTTTCTCTCTTACGAAACGCTCTTTAGCACTGGTGCCGCTCTCTCAGTTTCTCCCGCATTCATTCACAATTCCGTGAGCAAGGCGACGGGCAGCGAAAAGCTCTCTGGAGGGCACTTTTCAAATCAAATATAAATAAGTATGTGGCGGCTCCTCCGCGGACTCCTCCAAGTGTTGCATGTTTTCGTGAATGAAAACGAAAGTCTTTTGCGACCCGCCGCCAACTCGCAACCCCCCGTCCGCACCTTTGTGTGCACAACTTGGGGGTATATCGCCCCGAAAACCCAGTTCAGAGCACCCGATGTCGCCTCAAACCCCCCCTGGCCGCATCTAATTTGATTCATATATGTATAGGGGTATATGTACGCCTGTGTGACTTTGATTAGGTATTGGGCCAGCGAGTCGCTCTGATAACTGTCGTCCAGATAAGAGAGCTTTATGTCAAGTTCGAGTACTCCGT

GGGCTTCGAAACCCAAAACCGATATGCCATTAGCCTATCTACAGTGTTTATTCGAGGCATTATATCTTTATATCGCGTTCAGATATATGAGTAACTTTTAAACTCTCTAGCTTAAAATGTGGTAGAATCGCTTATGTTCAGTTTCTATGTTTGGTGCAAGTGTTTTATAAAATGCTAATCAGTAGCTTGCGCTATCTAAAAGTTTACGTTATCGAGCGGCTACTGTACTTACATATTTATGCTATTTTGTGCCAGTTAAAAATGAGTCAGGCTGAGCGCCAATTCAGCGATGAATAAAATTGTAGTATACTTTAAAGGGTGATTGATGAGTTGTAAAATCAAAATAATCGACTTTTAACAAGCAAACCCCCCTTGTTTTTTCCCTTTTTTCTTATCTTTACAGTCTGGCCATCAAGAATGCCGATGAAAATGCCCCCACGAAGCGAGATCTGGTAAATGCCGAATTCTTGGATGGCGAGCAGTGAGTTTGAGCACCAATCAACTTAATAAACTTAAGCCACGGAAAAACTACATTTATTTGTACATTACAGACCCACACTGGAGGAAATCCGCAGCTGGGGCAAAAGCTTTGACAAGCTGATGAAAAGTACAGGTAAGCCATTGACAATTGCCACCTTGATCCAAACTAATTGGTGGTTGGCCGAATATTTTCCCAGCTGGTCGAAAGGTGTTCCAGAACTTCCTGCGCAGCGAATTCAGTGAGGAGAACATCCTATTCTGGCTGGCCTGCGAGGATCTCAAGAAGGAGAATAGCCCAGAGTTGGTGGAGGAGAAGGCGCGCCTCATCTACGAGGACTACATCTCGATCCTGTCGCCCAGGGAGGTGTCGCTGGACTCCAGGGTCCGCGAGATTGTCAACCGGAACATGATCGAGCCCACGACGCACACCTTCGACGAAGCTCAAATCCAGATCTACACACTGATGCACAGAGACTCATATCCGAGGTGAGTGCTCTTTAGGCAAAAATAAAAGATACTGTATTTATACTTAAACGGGGTATCTTTTTCAACAGATTCCTAAACTCTCAAAAGTTCAAGACACTAGCTCAACTGCAAGACAACTCGAATGCCGGTT

 

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