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CRIMIC CR01614

Gene Prp8

CG8877

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:12161208..12161239	48E4	FBti0210456	86469

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGATGGCCAACCAAATTCAGCAGA	TCAACACGCTGCTTAGTCACCGTCTT

Images

Construct Design Sequences

sgRNA sequence	TAGAAAAACGACGAGCTCCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTAGCTTGGTCAGACGCAGG	TTGCGATAAAACGGGAGCTG	TTCTAACGCGGCATGAAGC	TGCAGGAGAAGGCGCTTAAA

 

Amplicon 5'

Amplicon 3'

TTAGCTTGGTCAGACGCAGGATCTCACGGCACAAATGGAAAGCTAATAGGAATGAAAGGAATTGATAAAAGGGAAATTTTTAGAAATTTAACTGCTTACCATTTCCGAATCGCGACTTCTTACGCTCCTTGGTGGTCAGAGTTTTAACGGGCTTCAAGTTGAAATTGTAATCTAAATGCAAGTAGTTAAGATTTTTTCGGTGAATCAATAGATTCAACATGTTATATCCCTGACGACAAACTTGCAGTCCAGCCTCAACCCAATCCAGAGTTGTGGTCTGGAAGAATTTTGTGGCCTTAAACGAACGGAAGAGGTAGCTGCAAAGTAAGGATACAATTCAAAACGTTTCTATAGTTTGATTTAAATAAAACTCACCGTTTCTTTTGTGGCTTTGGTTTGCGGTGTTTTAGGGCATTGAGCACATAGTATTTGAGCAGTTTCTGGTAGCTAACGCGCACCTTAACCGGATGGCCAGGTGGACAATGCTCCTTGTACCAGCACTTGACCAGCGGCACATCGATGGCACGACGGGAGCGACCAGAGCGCATGTTAAAGGGGCGGGGTGCCCACAGCAAGGCGATTCCGTTTGCAGTGTTGTCAGTGTACAGGGGAGTGTCCTGCAGGAACGGCTGCACGTCGTCCGGAAGAGTAAAGTCCTCGTCGTCATCAGGTAGGGGCTCCTGAATCTTCGAGTTTGCATTGCGATGGGATATGGGATTGATGAGCGGATCAAAGTAAAATGCAGGCAGATCGGGATCTTCGGTCTTGATGTAGACCACGTTGGGAGTGTGATACCTGAAATAGACAAAGTACATCATGTGGTCATGTGGATCCTCTAAGCTACAAATGAGTTTATATGTATGCAACTATCAGCAGTCACGATTAATTCTTAACAATCGCCACGAACATATTCATTGCAGCTCCCGTTTTATCGCAA

TTCTAACGCGGCATGAAGCATTTCCAGAGAGGCTGATCACTTGGAAACGTTTCTAACCACGTAAATAAGATTTTAGAATGGTAATTTTTGAAGAATGTGGACTCTTACCAGCTCAAATGCACAAAGTGTGGCATATTGTTGTACAAGTAAGGAAAGGCAATGCGGTATTCCGTGCGAATTGGCTGCCGGATAATGACTTTGTTAATGTCATTGAACTCGTTCCAATCCTCGTCCCTAAAATTAAATAAAAGCAGGAATTACATTTTAACAAGTATTTAGGGTATGCATTGTCTTACCCAACATTGTGGTCCTTAATGAGGGGTTCAAACTTAGGTCCTCCTGGAATGGCCATATTAAGAGCCTTGGCGGTGAAGAAACTTTTTGGGTCAAACAAATAAAAGAAGTTATTGTCGACTAGATCGGTGAGCAATTGATTGGCCAATCGATACAGCGTGGCTAGCTGAGGCAGGGAAAGATTCCACTTTCGGTAGGTAGTGCCGTTCACAAACCTTAGATGGTAATAAATGAATCAAAACAAGATCAGATATAAATGACAAATCAAAACTCACTGTGTATCCACTAGAGGACGATGGTCATAAAACCATTTGTATACAGCATTGTCTTCGTCATTGTCCAGCTCAATTTGAATGGCCTCCAAGGGCTCCACGTCCAGCACGTTGTCGGCATAGTCTAGAGGCGGCTCTTCATCGTCAAATGGCGGGAATCTCATGCGCTTAAAGTGGCGACGATCGCGTTTCTCACGCCGCATCATGATCCACATTGTACTGCGGAAGTGTTTAACGTCAGTTGTGTCCACGAGGACAAGTTAAAGTCAACTTACCCCCATTGTGCTATGTAGACGGGCTCAATAACCCAGGGAATCTCATTGACAAAGGTAATGGCTCCAGTGATGTGGTACAGCACCTGGACATCCCGGATCTGCTCCCATGGCATTGGCATGTTCTCTAGCAGCTTGAGCACAGCATGTGGCATGTACTTCAGGGCGCCGAGATATACTCGCTTGTCATGACGATACTTGCGCGACGTCATATCTCCGTGATCGCGAATGATCTTCCTGATGTGCTCCGGCGGCATATCCTCCTTCTGGGTATCCACGAAGCCAAACTTGCGCTTCTCAGCGAATCGCTTGGACTGCAGATGCTGCCATTTAAGCGCCTTCTCCTGCA

 

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