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CRIMIC CR01616

Gene Csp

CG6395

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:22268000..22268017	79D3	FBti0212937	91265

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	GCCACGCGTTAGTACATTTTGACCGA	TGATAACCACGCACAGGTAAGACCTC

Images

Construct Design Sequences

sgRNA sequence	ACCGGGTCAGGGGTAATAATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTGCACGACCTTTTCCTTG	CCTGACCCGGTTCCTTGTTT	GGTAGTGGGAAGGGGAGGG	AACCCGCTCTGAGAATCTGC

 

Amplicon 5'

Amplicon 3'

CGTGCACGACCTTTTCCTTGGGTGTTCCCTTTCACCCTTCATTTCTTAGACAATCTCGCTTCCGCCCATACAAATTTTATGCATACAGAAAATGACCTGCGGCCGATCGTTGCTTGTGAGTCGCTCACCAGAAAAGGTTATTGCGCTCGTAATAAAAAACTGTCGGATATTTTTTATTCCCGCAAGTAGAGTGTCAAAAAACACCCGGCTGCCTAGATACAACAGTGTTTAGCGGGTGCACTTGGAGTGCCAGTGGCTTGCTGCAGTTCTCTGGTGCACACTTTCGATGCGATTTCTTACAGTGAGTCCCGTAACAGCAGCTATTAATGTTGCGATAGATGGCGTGTTAGTCCAGAGCTGGAAATCATTGATGTCGGCAGGTAAACCGATAAGATAGAACATCGGTTCAGTTCCATCTCTAATTTCGTTGCGAAAAAAATAAGAATCGAGGCATCGGTACGGACGTCAAAAACTGCAATTATAAGAGTACACGCACAGATTGTGGTTCTTGGCAGGCACAGACTTTCACTGCGTGTGACAGCGATCGCTAGTGCAAGTTACCCGTTCGCAGTCAAAGTGACACAGGCATCAGGATGAGCGCACCTGGCATGGACAAGAGAAAACTGTCGTAAGTATTTTGTAGTGCTCCTTCATTTTTCCTCCACGGCGCACACACACGCGCCGTCGGCAGAGTACACACACTCAGCCCGTGCGCGAGTGTGAGTAGCAGCTGCGCGCAGGCAGCGCGGTCGGCGCGAAATTGATTTTTCGCGGCAGAAGTTTTCTCGAGCCAGCAGGCACTTTCCTTTCCACGTCGGGATAAACAAGGAACCGGGTCAGG

GGTAGTGGGAAGGGGAGGGCAGCTGCGGCTGGAGTTTGATTTCGGACAAGCGAATTCGTCCTGGGCAAGGCCAAGGACAAATTTGGCCATTTGACGGAATTCGATTTTTATTTGATTTCAATAATCGTTAGTTAAAATCTTAGTGGCATAGTTTCAACTTATGTTGCATTAGGCAGGAAAATGTAAAAGGTAGAAAAAAGGCTACGCACATCATCGATCAAGACTCGAGCCGATATCATTCAGATATGTGCTATGTAATACATACAGACCATTATTAGAATTACACATTATAACTACACAGTATGAGAATTTCCTCTTCCTTTCTCCTCTTCCTCTTGCATTCTTCTCGGTTGGCCACACCTCTTTTGCCCGCAAATCGCACGATTTCAATTGTTTAATAGAGAAAAAATGAGTCGAATACTTCATTTTATAAATATTGTTCTATTTAAATTGTTCAATGTTTGGGTCTTCGTGCAACACATACCGATTCCGGCATAAGTGCGCGATAACAACAGACCTACAGATCCAATAACACGACAACCAACTAACTAACCAGCCTGGCCGTTATCGGACAGAAAGTTTTACGGCCGCGTTAGCCGTCTCTATAATTGTACCCTATATGAGTAATGCCAGCACTAACCGCTTCAGTTTGGCCCAGGCGAATTCGAGTTGGCTTAGATTGTCATGATGCCTGCCAATGTCCTTGGCCCTGTGCAGTCGCCCACCGCCCAGTGTCCCTGGGGCACGCATCCGCTTGAGCCTGTAATCACCCTCATCATCGTGGTCCTCAACCACTTTGTCGAGCGCTCGCCGCCTTATCAGCTCGCAGATTCTCAGAGCGGGTT

 

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