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CRIMIC CR01618

Gene Nmnat

CG13645

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:24946369..24946395	96B11	FBti0210457	86470

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGAAAGCAATGTTCGTGTGGTACAC	GATGTGGACTCTTCCAAGGGAAGCAA

Images

Construct Design Sequences

sgRNA sequence	AACGATACGTACACTGAATGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATGCTTCAGTCCACCCACA	ACGTATCGTTTGAAAGATGGGT	CCATTTCACACAGCAGAGAACA	CTCCACTCCGTGAAGTGACC

 

Amplicon 5'

Amplicon 3'

GATGCTTCAGTCCACCCACACCCATGCACCTTCGGATGTTCGGTGAGTTAAACTAGATTGTGAAATTCTGCGGTACATAATCGATAAAATTACGCGGCATGTTTTTGTCAACAGAGATAGCCAAGGACCACTTCGAAATGCAGGGAACCCACAGAGTGGTAGGTGGCATCATCTCGCCCACGCACGACTCATATGGCAAAAAGGGTCTGGCCTCCGCCTTGGACAGATGTGCCATGGTCAAGCTGGCCACCCAGAGCTCCAATTGGATTCGGTTGTCCGATTGGGAGGTGCACCAGAATCAGTGGATGCGCACGCAGGCGGTGCTCCAGCACCACCAGAACTATATCAATAACCACATAAATTCCGGCGGGGGAGGCGGAGATGACGGGGAGAACACACATCTACCCGGATGGCTGCCTCGTGGGTTGCACGACAGTCGGGATCCTGTACATCTGAAGCTACTGTGCGGTGCCGATCTGCTGGAGTCCTTTGCTGTTCCAGGCCTATGGGCAGAGGCCGATGTAAGTCTTGATATATTTGAATAGTACCTTTTTTTATATGATTGTTCCCAGATTGAGGACATTGTGGCTAACCATGGTTTGGTAGTTATCACCCGGGCCGGCTCAAACCCAGGAAAATTCATCTTTGACTCCGACATATTGACCAAGTATCAGGTTAGAGAGACAGTACCTCGACAACATTATTTCTTATTCATTTCCATTCACATTTCAGAGCAACATTACGCTAATCACCAACTGGGTGCCTAATGAGGTGAGCTCCACGTTGATTCGGCGGCTCTTAGGACGCGGCCAGTCGGTCAAGTACCTTCTAGACGATCTGGTGCTGGAGTACATCAAGCGCCAACGATTGTTTAACTTTAAATCGTAGGTGGTCTTCCTGTTACCTCCCCTTAATCCTGCATGTTCACTTACAATTACCCATCTTTCAAACGATACGT

CCATTTCACACAGCAGAGAACACACTTAACGTGCACCACCAGCAGTCTTTGTATTGTTTCCTTTTATGTCTTAAACTGCAGGCGGGATGCACCGGCTCCGACCGAATGCGACTCTTGACGCGCTTTGCACGTGAAAAAGAAGCCCTCGTCGCCCACACGCTTTCCAACCCACCAGCACCGCAAATCGCACCACTTGCCCCACCAGCACCATAAGCTGTTTGAGCCGTGGCGCCTCTTCATGGAGCGTGAGTGAGCAGAGCAGATCCGAGTTCAAGTTCCTATTTAGCAGTGATTCCTTTAATGCATGTTTATTTTTGCCCAATCTTAGCCCTTGTAAATACCCTTGTTAAATTAGCCATAGTTTTGCGGTGGTCAGTGTGCGTATCATCTACTTACACCGAATTGTTGCCAAAATGTAGTCCCATTAAACAATAACCTTAACTTTGTTGCTATTGTACTGACCATGCCCGCCTAACCCATGGAATGTAGTAAGTATATAACGGATGCGGTGCGTCCCAACCATTTGCTCTTTAACCACGCCTATACGGACAACAACAAGAACGCGAACAGCTACTCTATAGGCGACCAGCTGGAGCAGGACATGGACGAGTCGGACACTCCAAGCCCGCAACTGCAGCATACGCCTACGTCGCGGGTTTTCTGCTGCGGGGAGGTGCCTTTACGTGGATCCAAGGTGCTGCGATCCGGTCCTGGACAAGCGGTGCAGGTCATCACCATGCAGGCAGATGAGAAAGAGGAGGTGGGTATCCTTAACCGCTGTATCCATTGCATTTCATACATAGATGGAATCCAGAGTTTTGCTTTCAAACTTATGTTTAGATCCTTCTAATCAATTCCCCTCTTTCAGAGCCAAGCAAAGAAGCAGAAGATTTCCCAAGTGCAACTTTAGGAAACTGGAAGACGAATCTCACGCGGATTAAGACAGGGGCGCTGGAAGCTATTTGTTGATATTATATAAATATGCAAAATAAACACGTTCGTTACCAAGTTGACGGCTGCCCATGCTTTTGTGGGTCACTTCACGGAGTGGAG

 

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