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CRIMIC CR01621

Gene Ephrin

CG1862

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	4:574641..574662	102C6	FBti0210458	86471

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGAATTCGTACACGGCTACAGCGTT	CGGCATTAGTCCCTACATGATTACCG

Images

Construct Design Sequences

sgRNA sequence	TTAATTTATCAATATATTATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTTGTCAACGTCGGAGCAT	TGGCCAGCAGTCTTATTATTGGA	TCCCTAATGTCGCGTATAAACAC	GCTCCTCCGGTGTCTGTAAC

 

Amplicon 5'

Amplicon 3'

AGTTGTCAACGTCGGAGCATGTTGGCATGTAAGAGGCGCTTAACTACCAGTAAAGTGCTTGAAGACTCACATCCACCAGTAGCTTTCCCCAATTGCAAATCTCACCGGCATCAGCAACAAAAGGAGAAACATAAAGTTCAGCTATACTCTGGAAAACCTTTAAGCATTAAGCTGTACGTGCCAGGAAGTATTGAGAGTATCCCAAAAATTCGACATAAGGCGCTTACGACAACGAATAAACAGCAACCAGCTATGCATCGAAAGTCTAAGAGCAAAAGCAAGTTTCAAGGTTTTAACAATCTCAAGCCGCTTTATTCACCGAGTAAAAGACAACCACCCGAGAAGCATTCATCAGTACTGGTGGAAGCAGGGATAGAATCAAAGGCATCACGGCATTTTGTTGGTAAAAAGAGGATTAAGAACAGAAACTGTTTGTTATCTTCGCCTCAGCCATCGCCAATGAGATGCAAAATGATGATTCCATTTCCAAAGTTTGGTGCCACATCCTTTGTTACGTTGCTCACTTTAATTTGTATGGAAACTGTTTTGCTCTCCACCATGTCTAGTTGCGCCAAGACTTTTTACATGCATTGGAACACATCGAACAGTATGTAAGTACTCAAATATAGTGGTATACTTAAGCAGCGGTATTATAATTTTATTGAAGTTAAAAATTGTAGATTTTTATTTTTCTCTACAAATGTATTATGTTTTATTACAATTATTACAGTTTTTAAGTCAGTAGTATGAGGATTATTATTATTCGTTTAGGGGATTTGTTATTTCAAATAAAATTTTCTTTCCAATAATAAGACTGCTGGCCA

TCCCTAATGTCGCGTATAAACACGCTGGGCTTTTATAAACTTTCCCAGCAATTAGTAATGCACATGAGTCATTTTCGATAGAATAACTATTGTCATAATTTGTTGTGCAAGTGAGCAGACGATGATTAGGCGATCCCATTGATCCCTAAGGATCATGATTAGTAACTTTTTTGGTAATCGCATCAGGGCATATATGGCCTACTCATTTTGTCTGGTTTTCTCTTTCTCGATATAAACATATCCATATCATCTATACCATCCATATTGCAATGGGACATTAAGTTTGTTAGGATAATGTTACTTCCAAAATCGGACAACAAACCCCAAAAACAGAAGACAAATGCACGTTTTCGTAGCCAAGGCATTAAACAATAAAGTCTAGATCAGATAAAAACAACCCAATTTTCCATTTTCAAAATGTTAACTTCAGCATTTTCATAAACTGTTGCTTAAACTATTCTGTGCGAACTATTTCAGCATTTTCCAAATCTAAAATTATATATGATATGTAGTTCCTTGAACCCTACGCGTTCAGTGCTCATCGTTACTATGTGTGATGTTTTGCACATTAATTGGCCCAAATTTGGTATTTCGATTTGCGCTTATTTATTGAAAATCGTTTTCTGACTTTCTCAAAACAAAATGTAATTTGATTGCACTCTTATATCCACAGATTTCGGATTGATAACACAGATCATATTATCGATGTTAATAAAGGCAATCTTGCATTTGAGTTCGATCAGGTTCATATAATATGCCCAGTATATGAGCCAGGGACTTTTGAGAACGAAACTGAAAAATACATAATTTACAATGTGTCTAAAGTGGAGTATGAAACTTGTCGCATAACAAATGCAGATCCGCGAGTAATAGCTATATGTGATAAACCTCAGAAATTAATGTTTTTTACAATAACTTTCCGGCCATTTACACCGCAGCCAGGTGGCTTGGAGTTCCTACCTGGAAATGATTATTACTTTATTTGTGAGTTCGTTTGCATTCCTTCTATTTAAAAAATAATATTTTTAATATTTATTTAGCAACTTCATCTAAGGATGATTTATATCGAAGAATTGGAGGTCGATGCTCTACAAATAACATGAAAGTCGTCTTTAAAGTGTGTTGTGCCCCAGAGGACAACAACAAAACCACGGCGCTAAGCAATTCTAAATCTGTTACAGACACCGGAGGAGC

 

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