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CRIMIC CR01622

Gene schlank

CG3576

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:6261757..6261798	5E3	FBti0212938	91266

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	TTCACGCACCAGCTGAGCATTGGAAT	ATAGGAAGTGCGTGTTGGGGTGTGTA

Images

Construct Design Sequences

sgRNA sequence	GCACTTGGGCTCGCGCAGCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGTATGTTGCCCCCGTTTA	GTAGCTGCCGTTCAAGCGA	GCACCGAGCAGCAGTTCC	TTCCCCAAGTTCCATCTGGC

 

Amplicon 5'

Amplicon 3'

GGGTATGTTGCCCCCGTTTAAAACTACACTATTCTATTCTAGGTTTAAATACTAAAGGGTTCGATGTTCAAAGAAATATATAAGAAAATATAAATATCCGTACATGCTGGCAAATTCAATTAGTTTATTACAATTCAGTTAGTTGAAAACCATTTAATTTTATTTAAAGATAAATTACCGTTGCCAAAACATCGATATGCACACCGGTGCATGGCTCGAATTTTGCACGCATATCGATACTTGATGCCATCGCTATTGCTGTACCATCGCTATGAACTACTATCCTCGAACTTGTAAACAACAAAAAGCCACTTCATCTAGTGACCATTTGGAGCTGCCCGTCCGTTTGGAGCTATTTCACCTACTGGCTACTGTCACCACCTCACACTGAACCGCTCCGAAATGGAATCGCCCAGTGCCCGAACTCTCGGGAATTCCCTGGGCGATGACAGCGGCAACGGGAATGAAAACGGGAATGGAAGTGGAAACGGAAACACCACAATGATGCCGCATGGAATCTACCATGAGCGGCAGACACGTCACCTGTGCGGCCTCCACGCACTGAACAATCTGTTCCAGGGGCCCGACATGTTCTCCAAATCGGAACTGGACGACTACTGCACCACATTGACCCCGCGCAACTGGCTGAATCCGCATCGATCGTGGATCGGTTGGGGCAACTACGACGTCAATGTGATCATGTACGCCCTGCAGCAGCGGAATTGCGAGGCGGTGTGGTTTGATCGACGGCGGGATCCACACTGTCTCAATCTGAGCGTCATTTTCGGCTTCATTCTCAATGTACCGGCTCAGATGAGCCTGGGCTACTACATCCCACTGCCCTTCCACATGCGACACTGGCTGGCACTGCGTCGCTTGAACGGCAGCTAC

GCACCGAGCAGCAGTTCCTCGAGTTTCTGGCCACCCAACTGCAGATGGATCACGAGCTATTCCTCGTGTTGGACGAGGAGACGGACTGCAAGGATAAGAGCCAGCAACGTTGGCTGCTACCGCAGTTCAGGGATTAATGTTATGGTTAGCACTCCAAATAAAGCTAAAATGGAGCAGTGCATTAGTTAATTGTACCAATTATTTGATGAAACTAACCAAAAAGTGCCCTAGAATATGCAAGTTAAACCTTTACCATCCATTTTGCACTGCAAGTGGAAGCCACCAAAATTATGTATGTAGTAGTAATAAAAGCGACAAGAATAATTCACATGTGCCATCAGTATGAACTTTTGAACCCTAGTTCTGTGCAAGCCATCATTTTCTAGCCAATTCATTAGAAATTTTGGACCAATCGAGAGCTGTGCCTTGCGGCAAAACGCCTCAAATTCGTTGGGTTTCGTGCGAATAATGATGGTGTTTTGATTGAGCTGCAACGCGATGACAGTTTGCGATTAGAGCCACCAGCAAGCATTATTTGAACGCATTTATTAAATTTCACTTAGGCAATATGCACGAATTGCGCTCAAAGTGCAATGCACTGGCAATTATTCGGCTCTTATCGCCGGCACAACTAAAATTTCGGAGCCCACTGGCGATCAAGCTGTACAGAATATATATATATATATATATATATATATATATATACTATATAGCATATATGTCGCCTGTTGTTTGGCGAATTACTTGCGCTTGTCAGCGATAATTCAGCACGCAAGGCAAACCGACGACGATTGTGAGATTAGCCCAAGTGAGTGCTATGGGCTATATCCTATATGTGGCCCGACTTGGGTTCTTTGGATTTTGGACGACGACGTCTGACTCACGGCCACCGATTGCCAGATGGAACTTGGGGAA

 

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