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CRIMIC CR01623

Gene AdamTS-B

CG4096

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:5705892..5705906	5B1	FBti0210459	86472

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCCTCCATGTCATTCGCAGGACTCT	ATCTTGTACCGCTTCCGTTCGCCAAT

Images

Construct Design Sequences

sgRNA sequence	GGCGACCCACAGCCACGGACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATTAAAGCTGCTACGCGCG	CGGCCACTTGGAAACTGGA	GGTCGCCTACTGTGTCAGAG	TGTACTCATCCAAGGGCGTC

 

Amplicon 5'

Amplicon 3'

AATTAAAGCTGCTACGCGCGAGGCAGGAGGAGCATGTGTAACAGGTAGCCGGCAGCGATTTGCATTGCCACCTGCGGCGCCAGACGTTGGCCAATTCGCATGCCCCACGCGCAGCTAGCCAAATGCGATTTCCGATTGCCCAGCCCCTGGGCAAGATTTATGGCCAGACCAATTCGTTTAATGAATGAATCCAGCCAGCGAAGTGAGCCAGCAGCCAAGTTGCTCGATTTCAAACGAGGCGGCTAAGTGGACGCGACGGATGCTTTGACACCTGCGTGTGTGTCTCATTTTTAGCGACAATGATCGCCTGCGTTGCATATTCCGATTCAGATACATATTCTGATTCAGATACAGATTCAGTGCCGAGATCAAGATTTATATGTATGTACCTCGTGGTGGCCCGGCGGCTTCCTGCTCGATTCAAACTCAATTAGTCGAGGCACCGAGATCTCAACAGAGATGACCGCCCAGTCACTGTCCAGTCACTGTCCAGTCACTGAAAGTCACCTAATGTCACGGTTTCAAATGAATCTATGCCCAACCATGAGCAACTTAACAAACTTCGATTTAAACACTACGTTTGAGTTTCACAATATGATAGTACCAAACTTTGTTTTATAAGCTAAATATGACTAAGAACTTGTTGGTTAATAATCGTAGCATAGTTTTTGGCGCTATGTTGGGGTTTGTTGTACTAAGTTGGAGCCACAAAACTTTCAAGGGGATGCACAAGTCATAGTCACAGGTTACAAATTATTGGATATTTATGCAATAATTCCATGTTACTGTGCACTCTCCTCCTCCACCTCCTCCTTCTCCTCCTTCTCCTCCTTCATTACCTACAAAAAATGCTCTATTGCCGGCAATTAGACGATTACATGTGATTCCATGTGATTCACCGTGATCACAGCATCGAATCTCGATCCGTCGGCACACCCAGACACTACGACTCATTCCAACTCACACCCAACGACAGCCGGGTCCGGTTTTCGTTGACTTATCGGGTGGTGGGGTTGTGGGGCGACGAAGAGCTGCGGACTTGGATCGTAATGGATCTGATCGGATAGTATGGGATTGGATTGGATTGGATCGATCGTTCGTGCCATGTTGCCAAGTTTTCCAGTTTCCAAGTGGCCG

GGTCGCCTACTGTGTCAGAGCACACTGCGAGAAATCAGATAGCTATATACTATATATACTATACTAAACTATGAACTAAATTGATTACAACTACCTATGTATGTATGTACCTTTATGAACTCAGTTGATTAGTTCACACTAGTTGTTTTTTCCACTGATAAGCAATATTTTTTTTCCAACTCACTCCGCTTTTCTGCCAGTGCAGCCCAGGGGCCGTCAGTCAGTCTAGCGTGCTCGGTACGCGATTGTCTCACCGCCCCCTCGGCGGATCCGACCGCTCCGATCTCCCCGATCTCGCCGATCTCCACGAGCGACCAAGATATAGTGGCGATGGGTTGTGTAAGCCGACCGGAATCGGAATCTCTCTGGGCGGGGGAAAAAAAAAACAAAAACAGGTGGTTTGTTTTGGTTTCGCTTATGTGCTATTAATCGGATATAGCTGCTTGTTTACCAGCGATTTGCTCCAAATTGGCTATTATTCCATTGAAATAATCAGGTTTTGGAGCGATGTGCTGTCTCACTGACAGTTAGCAACTTTGTTTCCATAATCTCCTATGTCTTTTCCCAATAACCAAATGGATTGTTCTTTTTCCACAACAACAGCTTCGGGATGTTCCACGATACGGCGAAGATCGGCTGCCATCCGCGCGTGGGTCCCATTGTGCACATCATGACGCCCACTTTTGGGGCGGACACCCTGCAGGTTTGCTGGTCGAACTGCAGCCGCAAGTATATCACACATTTCCTGGAGTAAGTATCTTCTTATCGGTTTGTATGCTCTGTAGCTGCTACTGGTTATGAAACTCCATCTCACAAACTTGAAAACCTAACGATTGCAGTCAGGGATTGGGCGAGTGCCTGGACGATCCGCCGACGCCCTTGGATGAGTACA

 

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