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CRIMIC CR01624

Gene ZAP3

CG32685

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:10077733..10077740	9B1	FBti0210460	86473

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAACAATGGTCCACCTGGCTTTGGAC	GAGCCAATGCTTAGCGATCGGATGTA

Images

Construct Design Sequences

sgRNA sequence	GGGTAAGAGGGGGGATCGAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCATTTGCAGACGGACGACA	CCCCCTCTTACCCACATTTTTG	GCGGAATAGTGCCCAACGA	GGGCTTTGGCAACGATGATC

 

Amplicon 5'

Amplicon 3'

TCATTTGCAGACGGACGACAAGTTGATCAAAGAACGAAATTGAAAAAGGATCTCCCCCATGAACGTTGTGTTTTGTCTTCCTTTATTGATATTAAATTCCATAGTATATATTGTAACAATTCGACTTAAATAATTTCGCGCTATAAAATTATTACATAGTACTCGAATCGAGAGATTTCTGTTTGCAAAGCAAAGAAGTTACTTATATAACTGCGTCGATTTGAAACGAACAAATAATATATAATAATTAGGTGAGAGCTTGGTCTATTTCTAAGTGATATTGCAGGGCAGAAGAAAGTAAAAATAGTTTATACCAAGGAAGCAACAAAATTAGATCCATACATGACGTTTTTTTTTTTTTTTAATTTTTTTTATACTCTTTTATTCGTTTTCTGCGAATGTGGCAGATGCGTATGCGTGATAATCTTAATATTATAGATCTTTCTCTGACTTCTGTACGCTGCATGTATATATAGCTAGTTATATATATTTAAGTATGTTTATTTTCGAGGAGTTTTCACGATCTGCCCTCCCTCGGATAGCCGCCCCCCAAAACAAAGTCTGTCCTCTGTCGCCTAATCAGATCCCGAATTCGAGTGTTCAGAGTTCAGAGTTCAGAGTTCAGAGTTCAGAGTTCAGAGTTCAGAGTTCAGAGTTCAGAGATCAAAGATCAGGTCCTGCGCTGCTGCTGCCGTTGCTGGAGCTTCCTACCGCCCCCTTCTCTTGCTGCACAGACAATGGATCAGACACAGATTGGTGGATATATGCATATATGCATAGGTATCGGGAACATCGCCAACAATCAATCAATACCACCGTTTAGATATTCATTTGCGTGTTGGTTTTCGTATTCGATTTGGTTATCGTTCGTTTGTAAGATTGTTTTAATTATATTTTTATATTCAGTATTTATATATCAGTATTGCATAAAAGTAGAAGAGAAGAAGAAAAAAACGGGGTTGGAATCGAAATTCCAGCATTAAAGATAACTAGAAAATGGCCTGGACAAGTTAACTAAATTAAATTTGATGATATCAATACAGATACAGATAGAGAAAGTGAAGGGTTACAAAAATGTGGGTAAGAGGGGG

GCGGAATAGTGCCCAACGAGAACGATATATATATATATATATATATATATATATATCTATAGGCTATCTGAACATCAGGCCGTTTGAGAAGGCATCCCACGGATATGCGTAATGTGAATAGAGCTGAATAAATAAGCATAGGAAGCAGATTGTCTACTTATTCGAAAACGTTGCATCAGATATTTCAGATATTTGTTAGCCCATCGATAGGCGATAGTACTGCGTACATTCGGACAAACAATCATTCGACGGCCCACAGGTTTTTGATAATGTTTTGGGTTTGGGTTAACCCCCAACGCATGGATTGATTAGGCATAATAATGGACTGATTATTCATAATAAAAACACTTAAAACAGTAACTATAAGTGCAAATAGCATCTGTACTTAATACAAAATCAACGAATTTACTGAATGGATAATGTTGTTTAAGGTTGTTTTTTTTTAACGTTTCAAAGATATGCATGCAGCCCTACTTGAATTTCCCCAAAACAAACATATTTCCAGATTGTACCATCCAAATGAATACTAATAGCAAGTGCTCTTGAGAAGATAACGTTCCCATATCTTCCTCAGACAATCAGACAATTGATATTGGAATTTGGCAGAGCATAGTGGGAGGGGAAAGAATCATCTTTCCCCGTTCCACAATTTTTTGTCAAGATATAACATTTCGATATTATTGTATACGTAGTGAAAAGATATTCAATCAAGTCGAAAAAGTTAAAAACAGATTGAAGACACATACCCGTCATTCCAGTTCTTCCGATTGTTGTTTCCTCCTCCTCCTCCTCCACCACCACCTCCGCCTGGATTTCCAAAGCGGTTGCCAAAGTTTCTGGGTCCTCTCGGTGGTCCTGCGCCCAGATCATCGTTGCCAAAGCCC

 

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