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CRIMIC CR01625

Gene CG6048

CG6048

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:5953049..5953064	5C10	FBti0212939	91267

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GGTTCAGGCTATACAGCGTACATTGG	GTCACTCCCATTCATCGAGCTAGTGA

Images

Construct Design Sequences

sgRNA sequence	GTCGAAGAGGTGGAAAAGTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTTTGCACATGACGCCTTG	ACCTCTTCGACTGATTTGCA	TCCACATTCAATTGTTCAAGGGA	GAAGAGTCAGACTTCCGGCC

 

Amplicon 5'

Amplicon 3'

TGTTTGCACATGACGCCTTGAGTTCATGTTATATCTTAATTAAAAAAGCACACTGGACCCTCATTTCGCAATTAAGATCGAAGATATATGGGGACTGATTTGAGAATTATTAGGAAATTTGGATGACAAATGATTGGGGGGAGTTATTGTCTGATATACAAGATCGTTGAACCACGTTAAAGAAAAAATATAATAAATATATAAATAAATAATTGCTATATATATGAATAATTGAATACATAAAGGTAAAGTTGTGTTTAAAAAAAAAAAAAAAAACAATTTATGTAAATTTGTATTAGCCAGAAAGACTCCAAAGATCTTGACAAGATACACAGATCGCATTTAATTAGTTTTGGGCAAACTTTTCAGGAAATCCACTGGGGAACTACCGTTTTTCAAGGGGCTCATAAATCGGTGTGGTAAACCCAAAGATTGGTCGCGTGGAAAATCCAGCGGTGGCACTTCAAACTAAGGCTGCAGTTCGTCACACCCGGACGGAGGTCTTCGGTTCTCCGGCGATGACAAATCAGTCCGGTGTTTTTTGTGGTGTTCTATTGAATTGTACGATGTCTATTGATTAGATTAGGATGCCAGATTTTTATTTTGGCGGCGATATTGGACAACTGGGCTCGGCTCCTTCATTAGTTGGTACCGCCACGTCGAGGAGTTAGCATATAGATTGGCAGAATGTTCGGATTGGGACAACTTTTGGCGGTAGCCCTTCTTCTCATTTTCTTGCCATCGGGCTTGAGAGGTTTGTGGGAGATTTATTAAATGATGGAATATTTCCTAATTGAAAACCTTATTTGGTTTTAAATCAGGCGCAACGACACGAACACACTTGGACACGAAGGCCATCCGTCCGAGATTCAATGCGGATCCCGGCAGGATTATCAATGGAACAGAGGCCTCTCTGGGCGCAACTAGGCATCAGGTGGGCATTCGCAAGGCTCTCAACGATGGCTACTTCTTTGGAACCGGGCACCTTTGCGGAGGATCTCTCATCCGGCCGGGATGGGTACTCACCGCCGCCCATTGCTTTGTGGAGTAAGTGGGTCTATTTCTAACTATTTTGGGCTACTCATTGAGAATGTGCCCTAAAATAATGTGAAATTCAAATGCAAATCAGTCGAAGAGGT

TCCACATTCAATTGTTCAAGGGATGCAGAAATGTGGCTTTGGGGTAACCTTTTAGCTTAGAGATTAGACTTAAAAATTTCTTTGAATTTCATTAGAATTAACATGCAATTTAACTAACTATTCGTTCTTATCATTAACTTTTGCTTACCAGTCAGATTATTTACGACGGCACTTTCGTGCCCAAGGAGGAGTTCATCGTGGTGATGGGTAACCTGGATCGCTACAATCGCACCAACACGCTAACATTCACGATCGAAGAGCGCATAATGCAGCTGGATAAGTTCGACCTTTCCACCTACGACAAGGACATCGCCCTGCTGATGCTGAATGGTACTGTACCCACTGGACATCCGACCATTCGTCCAATTGCCCTCAATCGGTTTGCCATACCGGAGGGCGTCGTCTGCCAGGTGACGGGCTGGGGCAATACGGAGGATGGCTATGTCTCCGACATCCTGATGACCGTCGATGTGCCGATGATCAGCGAGGAGCATTGCATCAATGACAGCGACCTGGGCCACCTGATTCAGCCGGGAATGATATGTGCCGGTTATTTGGAGGTGGGAGAAAAGGATGCCTGCGCCGGCGATTCCGGCGGACCATTGGTGTGCCAAAGTGAATTGGCCGGCGTGGTTTCCTGGGGCATTCAATGTGCCCTGCCCAGATTACCGGGTGTGTACACAGAGGTGTCCTACTACTACGACTGGATACTACAGAATATGGGCGAGAATGGTGAAGGAAGTGGTGAAGAAAGTGGTGAGGGAAGTGGTGAGGGAAGTGGTGAGGGAAGTGGTGAAGGAAGTGGTGAAGGTAGTGGTGATGACGGAAGTGGTGACGACGAAGATGGAAGTGGTGATGGTGGTGGTGCCATGGCCGCCGTGGCCGGAAGTCTGACTCTTC

 

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