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CRIMIC CR01626

Gene Ggt-1

CG6461

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:18509375..18509401	17C1	FBti0210461	86474

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAGCACTGCACCATACGCTACTACA	TGTTATCCAGCTTGGATTGGATGGCG

Images

Construct Design Sequences

sgRNA sequence	TCAACTGCGGCCGTAGATTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGCACATCCGATGCCATTG	TGGGCAGCTACTGAATCTGC	GCCGCAGTTGAGTGATTGC	TTATCTTCGAGCTCCGCTGC

 

Amplicon 5'

Amplicon 3'

CAGCACATCCGATGCCATTGGACACCACGGCTCCGCTGATGTACAGCGTGTCCCGATTCTTCAGACCGAAGACCAAGCCCAGAGTCAACGCCGTCACCATGAGGGCAGCCAGCAGTAGCCATAGCAAGAGCTTCTTACTCCACACAATCCTGCAAGTTGAGAATCTCATTAGAGTCAGACAATCAACGGATGCGATCGCGATCTCGATCGCCATCAGTGGCCCCAGGCCCTCAAAACTGAAGAGCTGGAGGACCTGGTCGAGTGGAGTGGTGGATCGGCGGTGTCGATTAGCTTTGATTTTGGACGCCGCTATCGGAAAACAGCCAATTCGCCGAGTCACTCGCTGGCAGGCCGCCTCGACACGGTGATACCCTACATTTCGATTTACGAACATGCTTAAGGTAGACAAGCTCATCGCAATATGTTAGTAATTCTTAAAGTTGAAAATTCTAGATGCAAATCTTCTACTGAAACGATACTACTACTACACAAGATAAAACTAAAGAAGCGCTTAACTGGTTTATATACACTATTATACTTTCAAAGCATAATATCTATATATTTAAGAGTAAGTCATAAATTTAAATAGGAGTCTAAAGTAATGGCTATACTTGAATTATGTGTGTTATTATATAAGATTTATAATGGTCACGATGGCCAGAAGTAAACAAAGTGTGAGATTAGAATAGAACGGTCTATTTGCCAACTTGATAGCCTGGATTGTAATCTCTGGACATGGACACTAGGGCACTAGTTAAATCAAATTAGCCGAATCATTGCGATCGATCTATCTGGAAAGCAGGTGAATGAAAAGCAGCTATCTGCAGATTCAGTAGCTGCCCA

GCCGCAGTTGAGTGATTGCCCCGCTGTGTGGAGTGTCCCATGACAAAGGGATATTCGTAGGTGCGCTTAGGCGCAGTCATGTCCACGTACCGGATGAGTCCGGACATACCCTCCACCATCTGTCCGGGGCGAGGGTGACGACTTATCGGCAATGGATGTGGAGTGCCCAAGTCTTCGATAACGTGCCATAAAAATGCCAAACAGAGATCGTAAAACTCAAGCGCTATTAACAGGCAATTTTCTATAATTAACGTAGATTTCTACGGGGGGAAAGTCGCAAGTACTTCGATTTAGTGGCCGTAACGCCGAAAAATGCGTCCTCCACATTCGCGATCCCAAATATTTCGATCGATACGATCGATTATCGCGTCCCGTCTGCAGATTAAGATCCTCAAGGGCCATCGCTTTGCAAATCCAATGCTCCATTTCTGCCCCGTCATTCGGTATGGTAAACCAACTGGATCTGCATATTATTATTATTATTATTATTATGTGCGGTTATCGTTCGACGATAAGATTCGGTTCATTCAGGCACTACACTCTCACTCCTTGTTACTTCACCTTTAATGGATTTACTACCATCGGTAGCATTGTGCTGCCTCCAATTGATCGGCAATTGTGCCATTGGCCAACTGGTCGGCTGGCTGGCCGGGATTATAGCCCCTTTATCCGCCTTTATCAGTGTGGGCATTTTCGGAATTTCAAAGTCGAGAAACCGTTGGCCGTAAAAGTTTATGGAAATGTTCTTTGAACCGATGGAGAAATAAAAATTCCGAAATATTCTCAAAACACTTGCGAATGCACTTGATTCTGAAGAGATCTTTAAACAACTGATAAAAGGTGATCAAACTGTTTTCACGGAACTCAAGTGCTGCTGTTTGTTATATGGCAAAGTACCCTTACCACATATGTATTTTAGTTAATATGTTACGGATGGCATCTTTTTTTTCCAAGATGAAAGCCTCCTGCTCCGCGAAGATATCTAGTGATCTCCGGAATGTTGACATCGTTTAATTACCAAAGATCGCAGCTGCGACATTGTACAAACGGTTTCAATCAGAAAACAGTCAGCACATTTCCATTTATCAGTGCTTTTTTTTCCCCTCGCCAAATGTGCCCCGTCATCAAAATCGAATCGTGTCTGCAGCGGAGCTCGAAGATAA

 

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