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CRIMIC CR01629

Gene Vps35

CG5625

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:22186418..22186437	58C7	FBti0212940	91268

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTCTACACCAAGACGATGGACAACA	TTTCTGAGCCAAGCTCAGCAGAGCAA

Images

Construct Design Sequences

sgRNA sequence	AAGCCCACTTTACTCATTCCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCTCAAGGCGTGGTTTAGT	TCCGGAAGTTGAGACGAAGG	AAAGTGGGCTTATCTCGAGTT	GGGAACACCTGGATGATGCA

 

Amplicon 5'

Amplicon 3'

CCCTCAAGGCGTGGTTTAGTAAGTGGCTGCACTGCCACTGTGGGGGCTTTTGGAAGCAGGTTCACTAGTAAATCTATGTGAGCAATGACCTTAGTTATTAAGTATGATTTAAGCTTCAAATAAAAGTACATGATTGCCAGCAATTTTAGTGAAATCAAGAAATTTAATATACTAAATATATTTCTTCTTTGCACGACGTGCTCGTTCAAAAAACTTAAGCCACAGAGAAGTCACCACCATACTTCACTATTTCAAAACTAAATTTATTTGAATAAAATATATTTTTTAACTCTATCTATGAATTGTGGTTGGTGTATTTGCTAACGGTTTGATATTAACAACAACAACATATCGAAGAACTTGCATCACTAGCATGTCAATCGATATCAGGACGTCCAATGTGTCTGTCGGCTGTCGGCAATCGATATCTCCTCTGCAAATAGATATTTTCAGAATAATTGGCTTATTTCACTTCGCCCTAACGCTGCAGTAATGGTAAGTGCATCGAGGTGCAACCTGTAGTCGCCAGAAGTCGAAATTCCTAGAGAACCGAGTGCCCCAGGTCCAGGCTTAACCGAGTGCTGAGTGCCCACCCTGTCGACGTCAAAAGAGGGCTGCCTGTGGTGAGAAGCAACGCCCCCCCGGTCCAAATCCCACGTCCATGTATCCCTGGAGCTCCTCCTCGTACGGCGGCCAGATTCCCAGTGCCGCCAACGTCCGGGTAAATACTCCGCCAATCCCAGATTACGAATCTTGGATATGTGTAATCGTTAATCCACTTATCTTCAATTTGGTCAATCCTTCGTCTCAACTTCCGGA

AAAGTGGGCTTATCTCGAGTTTATTTTATTTTTATATGTATATCTTAGCCAATAATATTGCTTACCATCTGTCCTTTCAGACCATGCCGAATGGTTTGGATGACCAGGAGAAGCTGCTCGCTGAAGCCATTGGACTAGCGAGGAAGCAGGCCTTTCAAATGAACCACTTTCTGGATAAGGAGCGTATGCTGGACTCGCTCAAGTGCGCCAGCACCATGCTGGGCGAGCTGCGGACCTCCGTCCTTTCCCCCAAAAGTTACTACGAGCTGTGTGAGTAAAACCAAAGCGTCTTTATACAACTTCAGATCTAATCGATTTAAATGAACCGCAGACATGGCCGTCACCAACGAACTGTGCCACCTGGAGCTGTACCTCAGCGAGAAAATCGACAAGAAAACGGATTTGTACGAGCTCGTTCAGTACTCGCATACTATTGTGCCGCGGTTGTACCTCCTCATAACAGTGGGCATTGTGTATATCAAGAATGACCCTACGCTGAAGCGCAGCATTCTAAAGGACCTGGTCGAGATGTGCCGCGGTGTCCAGCATCCGTTGCGAGGCCTCTTTCTGCGCAACTACCTGCTGCAGTGCACCCGGAACATCCTGCCCGATGTGATGGTTGAGGAGAACGAGCACGAGGGCAATGTCTATGATGCCATCGACTTCGTGCTGACCAACTTTGCCGAGATGAACAAGCTGTGGGTCCGCATGCAGCACCAGGGACACTCCAGCGAAAAGACGCGTCGCGAGAAGGAGCGTGAGGAACTAAAGATCCTGGTAGGCACTAATCTGGTGCGGTTGTCCCAGCTGGAGTCGGCCACTCTGGAAATCTACCAGCGACTCATTCTACCCGGCATCCTGGAGCAGGTGGTCAGCTGTCGCGATGCAATTGCACAGGAGTACCTAATGGAGTGCATCATCCAGGTGTTCCC

 

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