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CRIMIC CR01631

Gene sens

CG32120

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:13398410..13398425	70A8	FBti0210462	86475

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCAAGGACGAGCAGCAGCAGGATAA	CGATAGGGATCTGTGTGTGTGCTCAA

Images

Construct Design Sequences

sgRNA sequence	GCAAATTGACATTATGGCTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCGTGTATCGCTGTGGATGA	CTCAGGCAGTTGAGCGCA	TGCTTAGCTCTGACAACGGA	GGAAATCGAGGGCAAATCGC

 

Amplicon 5'

Amplicon 3'

CCGTGTATCGCTGTGGATGAGCAGATGTGTGGACAGGGTGGACGAACGCTTGAAGCTCTTGCCACACTGTTTGCACTAAAAAGAAAGACATAGACAATAGAAAAATACATTATTAACACCACATTCAGACAGAACGAGTCATGATTATTGGAACTCAGAATACCCCATATTTGCACAGCTTGAGCATATTCACACCAAAAAGCCAAATAATCAAAAAATCAGAAAAGAATTTATATATAAATTCACCGTTTTTGTGTGCCTGAGCCAAATTCGCAGTGGCAACATGAATTAAACTAGATTTGTGAGTGCTCAAATATTTGTTAGACAAGTGACGACAAGGGAAAACCCAAACCAAGCCAAACTTGGTTAGCCCACATCCAGGCGACAAATAATCTAGAACCTTAGCCATTTCTCAGCGCTGAAACCAGCTAGGTAAATATCTCGGCCCAATGCTAAATCTCAAGTGAGACACTTTCCAGGGAGCTTTAGCCATGCATATATGTACATACATTGTATATACCGCAGTATTTGTGGGCCAAACACAGCTGGGATCCACGGGGAATCGATCGCGGGGATTGGACGAATCCAGTGGCTGTAACATCTTCGGTTGTCAGATTAAATGTTTGAACAGTTTGCCAGTTGCAGCAACGTTGCAAGTAACCAAACATTGACATAACACAACTCAAATCGTCGGCCTACGTGGGCGACATTTATATTAAACACGAAAACATGAAAACAACGAAAAGTAAAACACAGAAATGGGTGAAAAAAATGTGAGACAATTTTTTTTTTGAAAAACCCGCATGGTCTACGCGTAATTGTAAATTGACACAAGTGTCGGTTTTTTAAAATTTCTTTCTTATTGATTTTTATGTTCTCTTATTTTTTTATTTTTTACGTTTTTTTTTAGCCTTGTTTTTGGGATTTTCTATTTGCGCATTGCATTCGCGTGGACCATATCCACTACACACATTGGCTAGACTCAGACCCAATTAGTTGCTCTATTGTTAAGCTGCGCTCAACTGCCTGAG

TGCTTAGCTCTGACAACGGAACGGAAGTTGGCAAAGCTCGAAAGGCAAAAAAAAAAAGAAAAAACGAGCAGGGAAACAAAAAAAGAGTTAAGGAGAAACCCCAAAGTCTGAGTCAGCCGGGTGGGCCTCCAATGGTGAACAGATGTAAAGCACGAATTACAGAAAAAATTCGATTTTATACATAAACAGCTGAAGAACAGTGACTGCGCCTGCGCAAAGGACTCTGGCAACTGTCTTCGTCGGCTTTTTGACCATATGTCGGTCCGGTACTGAAGCCCGAAAACCTATCGCATTTCACTCTGACCCTTTAATGTGTGAAAAAAAAACTGGAAAAAGATGTTAAAAAAAAAAAAAATAAGGACTCGCGGCGGGTGTTGATGGATATAAACGCAATATGACATCGGGGTAGGCATAAGGCCTATTAGGGTAAGTCGTCTCAAATTCCTGAGGGTCGTGTGCCCTCTTACCTTTTGCACAATCCAATATCGAATTATTATGATTGTAATTTGTACATAAATCTTCGAGGGCGAGTTATGCCCCCCACCGCACTGGCGCTAATCAAAATTATTTGATTAACTAATTGCGTGATTTCGATGGGACTTAATTGAATTTCCATAGAGCTCTATCGGTTTGGCTTGTATTACCAACTTTATGACAGTATTTGCTGTGCCACTTTTCCAAATTGAAAGTGGTATTGGTGGGGTTTTCGGCTCGGCGACAATTAAAACCCGCACGGACAAAATATGAAACAATTAACACAGGGGAATTGTCGAGGACACGCAACCTTATGCAATGATACGAACAGTATTCGTTTTGGGTTATAGATCATGGTCCTTCAGGACACGTGATAAGCCCATTAAAGCGGGCTGGGGGCTCAGAGATTTTCAAGCGATTTGCCCTCGATTTCC

 

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