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CRIMIC CR01635

Gene CG42346

CG42346

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:22874484..22874499	20F1	FBti0210463	86476

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATTCACTCGAATGCTTCGGAGAGC	AGATCTCGACGTTCATACGGACAGAC

Images

Construct Design Sequences

sgRNA sequence	TGGAACCATCGGCGCGGTGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTTCTCCTGCTTCAGCCCC	TTCCACTGGCTGCTGCAAT	AAGGGGATGGAGAAGGAGCA	TCGATTGATTGCAGTGGGCT

 

Amplicon 5'

Amplicon 3'

ATTTCTCCTGCTTCAGCCCCTGGGGTCATTCAGATACCGGCACCCATTCCGCTGCCCGTGCCGGCACCCATTCCGGCTGTCCTGTCTGTTTCGGAGGCACAACCGCGTTCCCACTCCCGTTCGCAATCGCACTCCCATCTCCATAGCCATTCCAGCTCCCATAACAATCCCCACCCGCAACCCGTTCCGGCCCCTGTTCCGGTACCAATTCCTGCTCCGATCGCCATGCCAACTCCTATGAGGGAGCCAAGCAGCGTGGTATTGGACCGCAAGTCTCGTAGACGCAGCTCCACCTCGCAGAACATCGCCTCGGCCGCCAGCAGTTCTATCGGCACCAACCAAGCCATCATCCAGTCTAGCACTATAACCATTACAAAGACGATTGCCAACGCCACTGGTATGGCCGAAACCAATTCAAGTGGCGGCGCTAGCATCTCCAGCACAAAAAATAAGGGGGACAGCAGTACCGGCCAATCTTCAAAGACGGTTGTCACCAATAACGCGGGCAGCAAGGCGGGTTACCAAGCCCCCAAGACTTCAGTGGCAAGCACATCTTCGAAAGCGGTGGTCAGGTCTAACGCAAGCGCCAGTTCAGGCCACGGGAACCACACCAGTTCCAGCTCGAGTTTCACCCAGCTGCACTCCAGTGCCAGGCCCTCGACAGCCAAAGATCACGACAAGAGTCCTCAGCAGAACCGCTCAGGCAGCTCAGGCCCCAAGGGCAAGTCGTCGAAGATCAACGATCCTCCACGTCGTGGCTCTTCACCGTCTGCTGGTGCAGAACCTACTCGCCAAAAACAAAGCGTTCAAAATCAAAATGGGCGGAATTGCAGCAGCCAGTGGAA

AAGGGGATGGAGAAGGAGCAGAAGCAAGTGGACGGGGCTACAGAGCCGTGGGTGAGGTAAACAAAGTGGCCTACAGAAACGGAAAGGACGCCTTCAAATTTCGCCAACCCGTGCGCAATGTTCGCCTGCACATTAAGGGATTGACGCGTCAGGTGACCAAGGAGCACATAACCGAGATATTTGGTCACTTCGGCGCCCTGACGGCAGTCGACTTTCCAATGGACCGTTTCCAAGGGCGCCAGGGACGCGGATACGCCTTTGTGGAGTACTCACGTCCAGAGGACTGCGCCAGTGCCATCAAGCATATGAACGGCGGGCAGATCGATGGGAAGCTGATCAGGGTGTCCGCCTTTCAGGAAAGCATGCTAAAGGCGCCGTGGCGTCAATACCGACGTTACTCGCCAGTCAACCGGCGCCAGAGCCAGCGTACGTTGTCCCGCTCGGGCTCGTCGTCACGCTCTCGATCGCCGTCCCGCTCTGCGTCCGGCTCGGAATACGGGTCGCGGTCCTCCCACTCTCGCGTCCGCTCTATGTCGGGATCGCGCTATTCTCGGCGCAACCGTTCGCAATCGTGCTCCTACACTCGCTCATCGCCGCGCTACGGGCATTACCATCGCCCTAGACGCGATTCGCGTTCGCCCTAGATAGGCGGACCTGTCTAATGTTGCAAAAGGAAGGCGAGATTAAGCCAGACAGGATTTTCAGGACTCTGCCAGATGCCTATTTTATTCCATTCCAACTTCAAATACAAATGTAGACTTGATGGACATCGTGCGTTTTAAAAGTTACTTATTAATACCAGTCTAATTTAAAAACATTGTTGGGCTTAAAATAAATAAAAATAAAAATCCATGCTCCGCACAAAAAAGAATTGTGAGGTATATCAGAAAATAATCATCGTATGTAAGGTGGTTATCTGTTAGTAATAATATAAAAGCCCACTGCAATCAATCGA

 

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