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CRIMIC CR01636

Gene CG8243

CG8243

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:8923200..8923213	44F7	FBti0217699

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GGATACTGCAGAAGATGTACACCCAC	TTGCTGCTGAAGCTGCTGCTGATGTT

Images

Construct Design Sequences

sgRNA sequence	TCGCAGTGCAGCTGCATGTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCACTGTGCGAGGTGAACT	TGTGTATATCCACGCACGCA	CTGCACTGCGATATGAGCCT	TCCAGTAAAGCCATGGGAGC

 

Amplicon 5'

Amplicon 3'

CTCACTGTGCGAGGTGAACTGCGCCAAACAATCCAATGCGCCATCCACCAGCTTAAGGAACGAGCGCTCCGGAGCTACTGCTTCGGAACTCGATTCTTCGCTGTAAAGATGCATGAGGCGCTTCAGGCACCAGCGAATGCGGTCCAGGAAGTACTGATGACTTACCTGCTTTCAGAGGATTAGACATCATTGTGCAATGCATGGATGAGATCCAAATTACTCACCGAGACTGTGGTGCCGCATTCTAGTTTCATGGTGTTCTCCACTTGGCGAATGCAGATCATAATCTGGCTGAGGCAGAGGCACACCAGCTCCGAGCACTCCGACTGCCCCTGGGAGCAGAAGGAGCTGTGGGCCAAAGTAGCGAATTCCAATAGCACGGCGCACAGGTCCTGAAATTCAAAGCAACAAGTGATTGATGTCCTGGGGCTAGTGGTCCGTTTCCCCCACTTACATTCAGCCAGTTTGCAGCTGCTGCATTGTTCCTGTTCAGTATCAGCAGGCAGGCCTCTAGTTTCGTTCGCAAAATTTCCATTTTTGCCGGCAAATGTGAGTTCAAATGTTCTGTGGGCAGAAGTAAAAGTAAACAACTTTTTTAAAAAGTGGCTTCGGTTCCCTGTGAATGGCAAGATGGCACGGCTTGGCGTTAGTGACAACCAGGGAGGCTAGCTGCCTTCACCGCCACACGGTCCCACTGGACGCATCACTTTTTCGGTAGCAAAAACTTGTAGAAAATTGTCAATTCAATTCGCTGGTAGTTGTATATCCATGTGAAGCAGAGATAAGCTCCGCCAACTCTACCCGTCGCCCACGCCACGTCGCACAGTTGCGCCCCGCCACAAATATCGAAACCAGGCCTCACAGACCGCAAGCATACCCCAAAATGAGCTCCTCGAATGCGGGACAGCGCACGAAGCTAATTCAGGAGAAGTGCCAGACCCTCCTGACGCAGATGCTCCGCGACGAGGACAACAAGTACTGCGTGGACTGCGATGCCAAGGGTATGTTAATGGGGTCCACTTCTCCGCCAAGAGATACGCCTCTTGGTGACGCACATCTGTGTGCGTGCGTGCGTGGATATACACA

CTGCACTGCGATATGAGCCTATGTGCTGTCACTGAATGCTACCTTTTATCCTTAGGTCCGCGCTGGGCCTCCTGGAACCTGGGCATGTTTCTCTGCATCCGCTGCGCCGGCATCCATCGCAATCTGGGCGTGCACATATCGCGCGTCAAGTCCGTCAACCTGGACACTTGGACGCCAGAGCAGGTGATCTCGCTGCAGCAGATGGGAAACTCGCGGGCTCGCGCCGTCTACGAGGCGCAGCTGCCGGACGGCTTCCGTCGCCCGCAGACGGACACAGCGCTGGAGAATTTCATCCGGGCCAAGTACGAGCACAAGAAGTACCTGGCCCGCGAGTGGGTGCCTCCCTCGCCGCCGAAAGTGGACTGGGCCAAGGAGATCGACGAGGAGCTTGAGCGCCAGAAGCGCAAGAAGAAGAGCACGCAGGCCCAGGCCACTTTGGGCCTGGCTGGAGTGGCCGGTGGCAGCGGCGACAAGCGCCTGTCCGGGTCATCTGCGTCGGCGCTCAAGAACACCCCCCTGCCCGCGCCGCTTCCAAAGCCGAAACCCAACCAGGTCGGCGGCTGCAGCCCAAAGACGACGCAACGCGTCCAGCTGAACAGCAGCGGCGTTAGCAGTGCCGGCGAAAGCGACCTCTTGGGACTGTCCTCGCCCACCAAGCCCATTGTCGCGACCAATGCCAGCCAAGACCTGCAAAACGAAAGTTTCACCAGCTTTCTCAGCGCCGAATCGATTGCCGGCCAGCCGGATAAGCCGATTGGCAGCAACGGCGACGCCGCCAATTTGATGGGCTCGAAGCCGAACTCGCTGGCACAAGAGGAGCAGGACTTCTTCAACCAGGGCTCACTGGGCGCCGGCGGCAACGAGAAGGATCAGTCTGGCAAGATGTCCAAGGACAGCATTCTGGCGCTGTACGGCAGCGCGCCAGCCACCCACAACCCCCAGATGAACTTTGGCGGCTTCACGGGCATGGCCCCAGGCGGCTATATGCACCAGCAGCAGCAACAGCAGATCCCCCACCAGTTTATGACACCGCCGAACTCGGTGAGCATGTATAATTCGCCAGTTATGGCTGCACCGAATGCGTTCAGCAATGCTGCCATCAGCAGTGCCACTGCGATGAGCATGGGCGGCTCCCATGGCTTTACTGGA

 

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