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CRIMIC CR01637

Gene GlyT

CG5549

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:23831404..23831413	60A2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TTTCCCTACCTGTGCTACAACAACGG	CCGCTTGGAAAAGGATACCGACTTTC

Images

Construct Design Sequences

sgRNA sequence	TTGCGAATTCCCCAGTCTGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATCCTGGTGTCCGCCATAG	GAATTCGCAAGGCAACGC	GCTGGTGACTTCCTTGGCTA	AGAGGACAAAGGCGAACGAG

 

Amplicon 5'

Amplicon 3'

GATCCTGGTGTCCGCCATAGTGATGCTCTACTACAACCTGATCATCGCCTGGACCATCTTCTACATGTTCGCCTCGTTTGCGCCGGTACTTCCGTGGCAGAATTGCGAGCCCGCCTGGAGCACAAAGTGTGAGTTTGTCCCCTGGGGAGCACATAGTGCGATGGATCCAATTAATTAATAGGCCAAAACACTTGGCGGGGGAAAAGGGGGGGAACGGTGGAGGATGAGGATAAGAGCGGATGTCAGATTCCCATCTTTTGTTACGTTTCTCGGGTAATAAATTAGGCAAATTGCAGGCTGTCAAAAGTGGAATTTTATTCCGCTCGGCTCCGCATTTGTTGTTTTTGTTTGTGTCCATCGGCGGCACGTGGCTCGGTTGGGAAAACACATGGCCAGGGAAATCGAAATCGGATGGCGGGTGCGGCGGGGAAAAATGGTTGTGTAACCAGCGGAGCCCGGAGACAATTGATTCGGGGCGACGCAGCAAAGATAAACACACACAATCCCAGGGAAACTTTCAAGGAAACTCGCAAGTCAGGGGAAAGAAATGTAAAATAAAAACTCGATAAGTTCGCTTCCGAAATATCCGAATAGTGGAGGCCAGCCTAGCATAATGCCACGGGGATCAAAAGTACATCATTCATTGTGAACAAATGTGAATGTCTCCACAATCCTGCCCTTCACGCACACGCAAGCAAATAGAAAGTATGAACAAACTTTCGCCCAGAATTATAATAATAAACAAGAAGCATGGAGGATTCCTAGGCGCCCTGAAGTAGGCAATTGTTTTTGTGCCTCGCTTGACATTGCAAGGTGAGAGCTTGCTCAGTGTGTGTGTGTGAGGACTCTATAAGTGTGTGTGTGTGTGTGTGTGGCGAAGGCGTTGCCTTGCGAATTC

GCTGGTGACTTCCTTGGCTAGAAACAGAGACACAGACACTTTAAAACGTTCCGCCACGCGAAACACATAAATCTTTAACGACCATTGAACGCCGACTCTTCCGGATTCTGAATTCCAGATACTGGAGCTGGGAGCTGGGAGCTGGGCGGCGGGTGGATTCGTCATGCTGCCCCGGAAAAGCGTGGCAAGTTTTCTATGTAGGTCAGAAGGGATGAGCTTATTTGTCGGGCATTCAACAAAATTTACATTTCCAGCATGTCATAAATGGTTGATTTTGTGAAGAAGTAGGAAAATCCAAATGAATGGGTGGAAACTTTCCTAATTGCTTTGATTAACAAAGGAAGAGTTATATAAATTCAAGCCTCTGCTATAGGATAGCATGATTTTTGAATATGGTTATGTAGGAATTCCTAGTACGATCCCTACCAAAATCAATACATAAGACGAAGGCACCTGCTTCGAAATCAAACTTAGACGGCGTTCCCTGACTGGCAGCCATTTCCATATTTTTTTCATTTTGTGCTGGCCCAGAAATCGATAATGGATGCTATTGAGGGTCCTGGATATTGAGCAGCGTCCCTCGACGCAGAGCATTTCACCTTCGGGGGAACGAACACGGCAGCTTCAAATTGTTAAAATGACAAACAATTATGTCTTAACTGTTTTTGACACTCAATCGCTGGCAGTGGGAAACTCAACCTTGGGCCGGGCTTTTCCCTAAGCTCCCAGGAATCCCACGACTAGAAAGTATTGTATGTGTATGCAGGGGAGGAGTGGGGTATGCGGGCGGAGTGGGGGGTCCTGCCCCGGCAGACAAGCAATTTCTTAAGGATTTTATTTATTGACACTTCTCGATTCGCCGCCCGATGTGCTGCTGCTGCCAGCATCCTGACCTCGTTCGCCTTTGTCCTCT

 

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